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991.
Droplet-based microfluidics has gained extensive research interest as it overcomes several challenges confronted by conventional single-phase microfluidics. The mixing performance inside droplets/slugs is critical in many applications such as advanced material syntheses and in situ kinetic measurements. In order to understand the effects of operating conditions on the mixing performance inside liquid slugs generated by a microfluidic T-junction, we have adopted the volume of fluid method coupled with the species transport model to study and quantify the mixing efficiencies inside slugs. Our simulation results demonstrate that an efficient mixing process is achieved by the intimate collaboration of the twirling effect and the recirculating flow. Only if the reagents are distributed transversely by the twirling effect, the recirculating flow can bring in convection mechanism thus facilitating mixing. By comparing the mixing performance inside slugs at various operating conditions, we find that slug size plays the key role in influencing the mixing performance as it determines the amount of fluid to be distributed by the twirling effect. For the cases where short slugs are generated, the mixing process is governed by the fast convection mechanism because the twirling effect can distribute the fluid to the flow path of the recirculating flow effectively. For cases with long slugs, the mixing process is dominated by the slow diffusion mechanism since the twirling effect is insufficient to distribute the large amount of fluid. In addition, our results show that increasing the operating velocity has limited effects on improving the mixing performance. This study provides the insight of the mixing process and may benefit the design and operations of droplet-based microfluidics. 相似文献
992.
AC Faradaic reactions have been reported as a mechanism inducing non-ideal phenomena such as flow reversal and cell deformation in electrokinetic microfluidic systems. Prior published work described experiments in parallel electrode arrays below the electrode charging frequency (fc), the frequency for electrical double layer charging at the electrode. However, 2D spatially non-uniform AC electric fields are required for applications such as in plane AC electroosmosis, AC electrothermal pumps, and dielectrophoresis. Many microscale experimental applications utilize AC frequencies around or above fc. In this work, a pH sensitive fluorescein sodium salt dye was used to detect [H+] as an indicator of Faradaic reactions in aqueous solutions within non-uniform AC electric fields. Comparison experiments with (a) parallel (2D uniform fields) electrodes and (b) organic media were employed to deduce the electrode charging mechanism at 5 kHz (1.5fc). Time dependency analysis illustrated that Faradaic reactions exist above the theoretically predicted electrode charging frequency. Spatial analysis showed [H+] varied spatially due to electric field non-uniformities and local pH changed at length scales greater than 50 μm away from the electrode surface. Thus, non-uniform AC fields yielded spatially varied pH gradients as a direct consequence of ion path length differences while uniform fields did not yield pH gradients; the latter is consistent with prior published data. Frequency dependence was examined from 5 kHz to 12 kHz at 5.5 Vpp potential, and voltage dependency was explored from 3.5 to 7.5 Vpp at 5 kHz. Results suggest that Faradaic reactions can still proceed within electrochemical systems in the absence of well-established electrical double layers. This work also illustrates that in microfluidic systems, spatial medium variations must be considered as a function of experiment time, initial medium conditions, electric signal potential, frequency, and spatial position. 相似文献
993.
Microfluidic devices allow for the production of physiologically relevant cellular microenvironments by including biomimetic hydrogels and generating controlled chemical gradients. During transport, the biomolecules interact in distinct ways with the fibrillar networks: as purely diffusive factors in the soluble fluid or bound to the matrix proteins. These two main mechanisms may regulate distinct cell responses in order to guide their directional migration: caused by the substrate-bound chemoattractant gradient (haptotaxis) or by the gradient established within the soluble fluid (chemotaxis). In this work 3D diffusion experiments, in combination with ELISA assays, are performed using microfluidic platforms in order to quantify the distribution of PDGF-BB and TGF-β1 across collagen and fibrin gels. Furthermore, to gain a deeper understanding of the fundamental processes, the experiments are reproduced by computer simulations based on a reaction-diffusion transport model. This model yields an accurate prediction of the experimental results, confirming that diffusion and binding phenomena are established within the microdevice. 相似文献
994.
Robert C.R. Siekmann 《体育科研》2014,(6):51-53
本文以法学视角,尤其是国际法视角,通过研读规则文本的方式,对足球规则的演进进行分析,并且揭示一些目前体育法领域关注的问题:体育法(lex sportiva)确实存在,包含了公法部分(国际公约、国家立法)和私法部分(体育规则);体育规则(Laws of the game)是体育法的核心,包括国家、地区以及全球范围内各级体育主管部门的章程与条例;项目规则是体育法的另一个重要核心,作者以足球为例,论证了为什么项目规则也是法的问题。 相似文献
995.
Road cycling performance is dependent on race tactics and pacing strategy. To optimise the pacing strategy for any race performed with no drafting, a numerical model was introduced, one that solves equations of motion while minimising the finishing time by varying the power output along the course. The power output was constrained by two different hydraulic models: the simpler critical power model for intermittent exercise (CPIE) and the more sophisticated Margaria–Morton model (M–M). These were compared with a constant power strategy (CPS). The simulation of the three different models was carried out on a fictional 75 kg cyclist, riding a 2,000 m course. This resulted in finishing times of 162.4, 155.8 and 159.3 s and speed variances of 0.58, 0.26 and 0.29 % for the CPS, CPIE and M–M simulations, respectively. Furthermore, the average power output was 469.7, 469.7 and 469.1 W for the CPS, CPIE and M–M simulations, respectively. The M–M model takes more physiological phenomena into consideration compared to the CPIE model and, therefore, contributes to an optimised pacing strategy that is more realistic. Therefore, the M–M model might be more suitable for future studies on optimal pacing strategy, despite the relatively slower finishing time. 相似文献
996.
Alexander H. Montoye Karin A. Pfeiffer Darijan Suton Stewart G. Trost 《Measurement in physical education and exercise science》2014,18(4):273-285
The responsiveness to change of the Actical and ActiGraph accelerometers was assessed in children and adolescents. Participants (N = 208) aged 6 to 16 years completed two simulated free-living protocols, one with primarily light-to-moderate physical activity (PA) and one with mostly moderate-to-vigorous PA. Time in sedentary, light, moderate, and vigorous PA was estimated using 8 previously developed cut-points (4 for Actical and 4 for ActiGraph) and 5-sec, 15-sec, and 30-sec epochs. Accelerometer responsiveness for detecting differences in PA between protocols was assessed using standardized response means (SRMs). SRM values ≥.8 represented high responsiveness to change. Both accelerometers showed high responsiveness for all PA intensities (SRMs = 1.2–4.7 for Actical and 1.1–3.3 for ActiGraph). All cut-points and epoch lengths yielded high responsiveness, and choice of cut-points and epoch length had little effect on responsiveness. Thus, both the Actical and ActiGraph can detect change in PA in a simulated free-living setting, irrespective of cut-point selection or epoch length. 相似文献
997.
Zoe Rutherford Brendan Gough Sarah Seymour-Smith Christopher R Matthews John Wilcox Dan Parnell 《Soccer & Society》2014,15(6):951-969
The purpose of this study was to examine whether an innovative, inclusive and integrated 12-week exercise, behaviour change and nutrition advice-based weight management programme could significantly improve the cardiovascular risk factors of overweight and obese men and women over the age of 35. One hundred and ninety-four men and 98 women (mean age?=?52.28?±?9.74 and 51.19?±?9.04) attending a community-based intervention delivered by Notts County Football in the Community over one year, took part in the study. Height (m), weight (kg), fitness (meters covered during a 6?min walk) and waist circumference (cm) were measured at weeks 1 and 12 as part of the intervention. Changes in body weight, waist circumference and fitness for men and women were measured by a 2-way repeated measures ANOVA, with significance set to p?<?0.05.Weight, waist circumference and fitness significantly improved over time in both men (4.96?kg, 6.29?cm, 70.22?m; p?<?0.05) and women (4.26?kg, 5.90?cm, 35.29?m; p?<?0.05). The results demonstrated that the FITC lead weight loss intervention was successful in significantly improving cardiovascular risk factors in both men and women. In particular, the weight loss reductions achieved were comparable to those seen in similar, more costly men-only programmes. This is the first study to demonstrate the efficacy of such an intervention in an inclusive, mixed gender programme and more specifically, in women. 相似文献
998.
Numerous studies have confirmed direct health effects of interventions with a physical activity component on students without providing sufficient information on the contents or the degree to which the programs have been carried out. The empirical evidence of the indirect health effects of physical education is weaker. In this article the focus is placed on the evaluation of the indirect health effects, such as the enhancement of physical activity and its psychological determinants (e.g. motivation, attitudes, self-efficacy and knowledge) of the health promotion program HealthyPEP, using a quasi-experimental design with 516 sixth grade students. When taking into account the contents of HealthyPEP and the degree of implementation, it was clear that the program had different effects on the two genders whereby girls evaluated HealthyPEP in a more positive way compared to boys. In girls, positive tendencies were observed on rational cognitive determinants (e.g. knowledge and attitudes towards sports). The contents of HealthyPEP must be further optimized in the future to achieve positive indirect health effects also on boys. 相似文献
999.
1000.
OBJECTIVE: The goals of this current project were to expand and elaborate on the results of Varia, Abidin, and Dass' (1996) study to better understand what historical parenting factors may contribute in creating a "Minimizing" perception of childhood psychological abuse. A second objective included examining the association between perceptual styles and adult ratings of adult relationship satisfaction. METHOD: Ninety individuals from a non-clinical adult sample completed self-report questionnaires regarding psychological maltreatment and adult relationship satisfaction. Three groups were created and compared using analysis of variance. The groups were (I) "Non-Abused," consistent reporters of no abuse; (II) "Acknowledgers," consistent reporters of abuse; and (III) "Minimizers," reporters of abuse, but do not label themselves as having been abused. RESULTS: Results indicated a continuum effect with the Non-Abused individuals reporting the highest level of maternal warmth and affection, the Acknowledgers reporting the lowest, with the Minimizers in the middle. The Non-Abused group reported the healthiest adjustment in terms of adult relationship satisfaction. While Minimizers and Acknowledgers reported similar levels of psychologically abusive experiences, Acknowledgers reported more difficulties with adult relationships. CONCLUSIONS: This study highlights the importance of studying Minimizers, a group which describes abusive events but minimizes the meaning and scope of these experiences. The relatively higher levels of maternal care which the Minimizers reported is hypothesized to be associated with their perceptions that they were not abused and acted as a protective factor associated with better quality adult relationships. The varying degrees of nurturance and care in the early family environment of abused individuals is speculated to be associated with distinct perceptual styles. 相似文献