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91.
Indu Verma Satinder Kaur Subash Goyal Shweta Goyal J. S. Multani A. P. S. Narang 《Indian journal of clinical biochemistry : IJCB》2014,29(3):382-385
The supply of oxygen is limited in certain intra abdominal conditions due to direct vascular invasion or inflammatory process, resulting in high lactate levels. Aim of this study was to find the predictive value of lactate levels in the peritoneal fluid (PF) and blood of patients with acute abdomen. The study comprised of fifty patients with acute abdominal conditions, admitted in emergency ward of tertiary care hospital, thirty patients were with surgical abdomen (group I) and twenty patients with non surgical abdomen (group II). Lactate was estimated in PF and blood on Blood Gas Analyzer (NOVA, M-7). The mean lactate levels in PF were significantly higher in group I as compared to group II (14.65 ± 1.195 vs. 5.92 ± 0.97 mmol/L, p < 0.001). There was no significant difference in blood lactate levels in both the groups. When PF and blood lactate levels were compared within groups, we found that PF levels were significantly higher than blood in group I (14.65 ± 1.195 vs. 3.85 ± 0.54 mmol/L, p < 0.001) but not in group II (5.92 ± 0.97 vs. 4.36 ± 0.95 mmol/L). Diagnostic value was obtained using ROC curve. Cut off values obtained for PF lactate, difference and ratio of PF and blood lactate (≥6.4 mmol/L, ≥3.3 and ≥2.1 respectively) are at very high degree of sensitivity and specificity. So it can be useful marker of surgical emergency in patients with acute intra abdominal pathology, especially in clinically ill patients or in whom physical examination is not yielding because of neurologic disorders or unresponsiveness. 相似文献
92.
Maedeh Roushan Parminder Kaur Alena Karpusenko Preston J. Countryman Carlos P. Ortiz Shuang Fang Lim Hong Wang Robert Riehn 《Biomicrofluidics》2014,8(3)
We present an analytic technique for probing protein-catalyzed transient DNA loops that is based on nanofluidic channels. In these nanochannels, DNA is forced in a linear configuration
that makes loops appear as folds whose size can easily be quantified. Using this technique, we study
the interaction between T4 DNA ligase and DNA. We find that T4 DNA ligase binding changes the physical characteristics of the DNA
polymer, in particular
persistence length and effective width. We find that the rate of DNA fold unrolling is significantly reduced
when T4 DNA ligase and ATP
are applied to bare DNA.
Together with evidence of T4 DNA ligase bridging two different segments of DNA based on AFM imaging, we thus conclude that ligase
can transiently stabilize folded
DNA configurations by coordinating genetically distant DNA stretches. 相似文献
93.
94.
95.
The use of ultrasound for promoting chemical reactions is described. 相似文献