Detection of enzymes dehydrogenases and proteases inBrugia malayi filarial parasites     

Y. P. Bhandary  K. N. Krithika  Sandeep Kulkarni  M. V. R. Reddy  B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2006,21(1):1-7

Lymphatic filariasis caused mainly by infection fromW. bancrofti andB. malayi remains a major cause of clinical morbidity in tropical and subtropical countries. Analysis ofB. malayi mf, infective larval and adult worm lysates for the activity of enzymes led to the demonstration of activities of three key enzymes of carbohydrate metabolism viz., Malate dehydrogenase (MDH), Malic enzyme (ME) and Glucose-6-phosphate dehydrogenase (G6PDH) in all the three stages of the parasite. The specific activity of all the three dehydrogenases was significantly high in mf lysate compared to their activity in lysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel to their activity inlysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel electrophoresis (PAGE) using 7.5% non-gradient gel showed the presence of two isoforms of each of the three enzymes (MDH, ME & G6PDH) in mf lysate, while only one form of each enzyme was present in L₃ larval and adult worm lysates. Further proteolytic enzyme activity was demonstrated both in microfilarial and infective larval lysates ofB. malayi. While both mf and L₃ larval lysates showed optimal protease activity at alkaline pH of 9.0, the mf lysate showed increased activity also at pH 3.0. The infective larval lysate was markedly inhibited by Tosylamide-L-Phenylalanine chloromethyl ketone (TPCK), a thiol protease inhibitor, while the protease activity in mf lysate was significantly inhibited by both TPCK and a serine protease inhibitor Phenyl Methyl Sulphonyl Flouride (PMSF). In sodium do-decyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), using gelatin copolymerized gel, the microfilarial lysate showed 3 protease molecules of 40 kDa, 180 kDa and 200 kDa and the L₃ larval lysate had 6 protease molecules of 18, 25, 37, 49, 70 and 200 kDa size.  相似文献   

Application of tumor markers in ovarian malignancies     

T. Malati  G. Rajani Kumari  B. Yadagiri 《Indian journal of clinical biochemistry : IJCB》2001,16(2):224-233

Ovarian cancer is the fifth leading cause of death in women. The incidence of this malignancy increases in women over the age of 40. The overall five years survival is less than 30%, as most women present with advanced stage disease. Until recently, detection of early stage ovarian cancer has been difficult since it is usually nonpalpable and asymptomatic. The definitive diagnosis of an ovarian mass is a common problem in gynecologic patients with adnexal mass. The routine standard evaluation for adnexal masses includes patient's history, physical examination, ultrasound and histopathological examination. These parameters individually or in combination have little predictive value. The accuracy of diagnostic tools are of immense value and great concern to practicsing Gynecologists and Oncologists. The clinical application of serum concentration of CA 125, AFP and hCG is of great help not only as diagnostic aid but also in monitoring efficacy of any treatment modality like chemotherapy, radiotherapy or surgical resection. Additionally, evaluation of tumor marker concentration helps in predicting early biochemical recurrence and in prognostication in different types of ovarian malignancies. The ability to differentiate a malignant mass from a benign pelvic mass pretherapeutically could be enhanced optimally by additional use of tumor markers such as cancer antigen CA-125, alphafetoprotein and human chorionic gonadotrophin in pre-and postmenopausal women.  相似文献   

Effects of axial conduction in laminar tube flow with convective boundaries     

B. Vick  M.N. Özişik  D.F. Ullrich 《Journal of The Franklin Institute》1983,316(2):159-173

Heat transfer in laminar tube flow with convective boundary conditions and axial heat conduction in the fluid is solved exactly. The effects of the external Biot numbers and the axial conduction in fluid on the temperature distribution and the local Nusselt number are determined for both the upstream and the downstream regions. In order to illustrate the effects of velocity profile, the results for the slug flow and the parabolic velocity profiles are plotted simultaneously. The heat transfer characteristics of the flow are found to be rather sensitive to the Péclét number, the external Biot numbers and the velocity profile in the thermal entrance region.  相似文献   

Do University policies make a difference?     

David B. Audretsch  Erik E. Lehmann 《Research Policy》2005,34(3):343-347

The purpose of this paper is to examine whether the technical universities have been more successful in facilitating the spillover and commercialization of knowledge. We compare the impact of technical and general universities on the performance of knowledge-based firms. Based on a unique data set consisting of publicly held high technology firms, the empirical evidence suggests that, in fact, firm performance is not influenced by the type of university. Technical universities do not have a differential impact on firm performance from more general universities.  相似文献   

Microscopic haematuria as an occult filarial infection in bhubaneshwar an endemic area for bancroftian filariasis     

R. Alli  B. Bhunia  G. P. Chhotray  M. V. R. Reddy  B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2003,18(1):61-64

Sera samples of 7 microscopic haematuria cases collected before and after treatment with Diethylcarbamazine citrate, (DEC), 9 microfilaraemic cases and 19 endemic normal individuals were analysed for filarial antigen and IgG antibody levels. Filarial antigen was detected in 5 of the 7 microscopic haematuria cases, of which 3 turned negative for antigen after treatment with DEC. While none of the 7 haematuria cases were positive for filarial IgG antibodies, before the DEC treatment, all of them turned positive after DEC treatment. The sensitivity and specificity values(to detect mf +ve cases) were 89% and 90% respectively for the detection of filarial antigen and 78% and 95% respectively for the detection of filarial IgG antibodies.  相似文献   

Lipid peroxidation and glutathione peroxidase in ischemic heart disease     

K. S. Motghare  Anil Bhutey  B. B. Murrhar  Madhur Gupta  A. W. Meshram  Y. Balsubramanium 《Indian journal of clinical biochemistry : IJCB》2001,16(2):213-215

With the growing interest in the concept of free radicals in the pathogenesis of myocardial ischemia, it was thought worthwhile to study the changes in lipid peroxides and antioxidant enzyme glutathione peroxidase (GPx) in ischemic heart disease (IHD). The study was carried out on 76 patients of IHD-38 of acute myocardial infarction (AMI) and 38 of stable ischemic heart disease (SIDH). They were age and sex matched with 38 normal healthy controls. A significant increase (p<0.001) in lipid peroxides as malondialdehyde (MDA) (5.9±0.7 mmol/L) and a decrease in GPx (24.6±2.2 U/gmHb) was found in patients of AMI when compared with controls. There was no significant difference in these values in SIHD. Thus this study confirms the earlier findings that MDA and GPx are useful parameters in IHD and their magnitude is dependent on severity and/or duration of ischemia. We suggest that these tests would be of use in smaller institutions with limited facilities.  相似文献   

Cataglyphis     

R. D. Harkness B.Sc.  M.B.  B.S.  R. Wehner Dr.Phil.Nat. 《Endeavour》1977,1(3-4)

Cataglyphis is an ant of desert regions and has a behavioural pattern comparable with that of the bee, but much easier to study. It has a well-developed system of foraging and navigating and a social organisation well adapted to its environment. This review, by two independent research workers who have devoted much time to field studies, is the first to bring together most of the existing information about these fascinating insects.  相似文献   

The small-gain theorem,the passivity theorem and their equivalence     

B.D.O. Anderson 《Journal of The Franklin Institute》1972

Two small-gain theorems and a passivity theorem yielding stability results for general feedback systems are shown to be equivalent.  相似文献   

死亡的脸     

含温·努兰  杨慕华 《中国科技信息》2003,(2):56

人们都说人应该有尊严的生,有尊严的死.然而在耶鲁大学外科与医学史教授舍温·努兰医生的眼中,很少有人能够有尊严的死去.  相似文献   

The periodical literature of demography and Bradford's law     

B. C. Peritz 《International Journal of Information Management》1986,6(3)

This paper presents a statistical analysis of the journals and papers abstracted in Population Index, 1984. A Bradford distribution is fitted to the papers in all the journals regularly scanned by Population Index, as well as in the journals specifically designated as demography. The fit of these distributions, and in particular the second one, is remarkably close. A core of journals for demography is identified. A breakdown of the journals and the papers published in them by the journal's field is presented and discussed. Data on the distribution of journals by country of publication are also given. An attempt is made to correlate the journal's productivity according to Population Index with the citation impact according to Social Sciences Citation Index.  相似文献