Effect of surface acoustic waves on the viability, proliferation and differentiation of primary osteoblast-like cells     

Haiyan Li  James Friend  Leslie Yeo  Ayan Dasvarma    Kathy Traianedes 《Biomicrofluidics》2009,3(3)

Surface acoustic waves (SAWs) have been used as a rapid and efficient technique for driving microparticles into a three-dimensional scaffold matrix, raising the possibility that SAW may be effective in seeding live cells into scaffolds, that is, if the cells were able to survive the infusion process. Primary osteoblast-like cells were used to specifically address this issue: To investigate the effects of SAW on the cells’ viability, proliferation, and differentiation. Fluorescence-labeled osteoblast-like cells were seeded into polycaprolactone scaffolds using the SAW method with a static method as a control. The cell distribution in the scaffold was assessed through image analysis. The cells were far more uniformly driven into the scaffold with the SAW method compared to the control, and the seeding process with SAW was also significantly faster: Cells were delivered into the scaffold in seconds compared to the hour-long process of static seeding. Over 80% of the osteoblast-like cells were found to be viable after being treated with SAW at 20 MHz for 10–30 s with an applied power of 380 mW over a wide range of cell suspension volumes (10–100 μℓ) and cell densities (1000–8000 cells∕μℓ). After determining the optimal cell seeding parameters, we further found that the treated cells offered the same functionality as untreated cells. Taken together, these results show that the SAW method has significant potential as a practical scaffold cell seeding method for tissue and orthopedic engineering.  相似文献   

Is the Secure Base Phenomenon Evident Here,There, and Anywhere? A Cross‐Cultural Study of Child Behavior and Experts’ Definitions     

German Posada  Ting Lu  Jill Trumbell  Garene Kaloustian  Marcel Trudel  Sandra J. Plata  Paola P. Peña  Jennifer Perez  Susana Tereno  Romain Dugravier  Gabrielle Coppola  Alessandro Constantini  Rosalinda Cassibba  Kiyomi Kondo‐Ikemura  Magaly Nóblega  Ines M. Haya  Claudia Pedraglio  Manuela Verissimo  Antonio J. Santos  Ligia Monteiro  Keng‐Ling Lay 《Child development》2013,84(6):1896-1905

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Constructive Use of Authoritative Sources in Science Meaning‐making     

Jennifer Yeo  Seng Chee Tan 《International Journal of Science Education》2013,35(13):1739-1754

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Referee acknowledgment for 2012     

Hsueh-Chia Chang  Leslie Y. Yeo  Editors 《Biomicrofluidics》2013,7(1)

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Introduction to the Special Issue: Multimodal Meaning-Making in Science     

Nielsen  Wendy  Yeo  Jennifer 《Research in Science Education》2022,52(3):751-754

Research in Science Education -  相似文献   

Editorial: A new year and a new Associate Editor     

Hsueh-Chia Chang  Leslie Yeo 《Biomicrofluidics》2011,5(1)

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Developmental Differences in Curriculum-Based Measurement (CBM) Reading Aloud Growth Rates Between English-Speaking Students and English Language Learners in Grade 8     

Seungsoo Yeo  Sohee Park 《Exceptionality》2014,22(2):91-110

The purpose of this study was to examine the developmental difference in curriculum-based measurement (CBM) reading aloud performance between Grade 8 English-speaking students and English language learners (ELLs) using two theories of reading development: compensatory model and cumulative model. Fifty non-ELLs and 133 ELLs were administered the same CBM reading aloud probes in fall, winter, and spring. The simplex model and latent growth model were used to identify the most appropriate reading model for each group. The results revealed that the compensatory model was appropriate for both groups. Limitations and educational implications are presented.  相似文献   

Measuring Children's Age Stereotyping Using a Modified Piagetian Conservation Task     

Sheree T. Kwong See  Carmen Rasmussen  S. Quinn Pertman 《Educational gerontology》2013,39(3):149-165

We examined five-year-old-children's age stereotyping using a modified Piagetian conservation task. Children were asked if two lines of objects were the “same” after one line had been made longer (transformed). A conversational account posits that children's answers reflect assumptions about the asker's motivation for the question (Schwarz, 1996 Schwarz , N. ( 1996 ). Cognition and communication: Judgmental biases, research methods, and the logic of conversation . Mahwah , NJ : Lawrence Erlbaum . [Google Scholar]). We reasoned that when asked by a young adult (puppet Experiment 1, experimenter Experiment 2) children would assume the question is to ascertain if they have noted a perceptual change and would provide answers focused on the change (length). If older adults are assumed to be in decline because of negative age stereotyping, however, children may assume an older adult (puppet, experimenter) is seeking clarification about line equality and would, therefore, give responses focused on the similarity of the lines (number). The expectation that a focus on length or number should differ across puppet/experimenter conditions was supported in both experiments and provides behavioral evidence for children's old age stereotyping.  相似文献   

Managing Knowledge in Early Modern Europe     

Yeo  Richard 《Minerva》2002,40(3):301-314

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Preface to Special Topic: Invited Papers from the 2009 Conference on Advances in Microfluidics and Nanofluidics, The Hong Kong University of Science & Technology, Hong Kong, 2009     

Leslie Y. Yeo  Weija Wen    Hsueh-Chia Chang 《Biomicrofluidics》2009,3(1)

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