Investigation of the Effects of Inulin and β-Glucan on the Physical and Sensory Properties of Low-Fat Beef Burgers Containing Vegetable Oils: Optimisation of the Formulation Using D-Optimal Mixture Design     

Roya Afshari  Hedayat Hosseini  Ramin Khaksar  Mohammad Amin Mohammadifar  Zohre Amiri  Rozita Komeili  Amin Mousavi Khaneghah 《Food Technology and Biotechnology》2015,53(4):436-445

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Determination of Biological Variance and Validation of a Fluorometric Assay for Measurement of α-l-Iduronidase Activity in Dried Blood Spots Samples: The First Experience in Iran     

Mohammad Abdi  Mohammad Said Hakhamaneshi  Mohammad Reza Alaei  Namam-Ali Azadi  Rahim Vakili  Daniel Zamanfar  Mohammad Taghikhani  Shohreh Khatami 《Indian journal of clinical biochemistry : IJCB》2015,30(3):318-322

Methods for assaying lysosomal diseases in dried blood samples are very useful today due to its several advantages related to the stability of samples, its transportation, handled and analysis, and its potential use for newborn screening compared to traditional methods in leucocytes samples. For this reason, it is important to validate these assays before being used in routine laboratory. Because of different in biological markers based on ethnicity, we aimed this study to validation a DBS-based fluorometric assay for measurement of α-l-Iduronidase activity for diagnosis of MPS I patients in Iran. DBS samples were collected from 15 MPS I patients and 60 healthy age matched subjects. Diagnostic value, biological variance and α-l-Iduronidase activity were determined. DBS α-l-Iduronidase activity was significantly higher in male subjects than in female group. Using a cut-off level of 1.08 µmol/spot 20 h, sensitivity and specificity were 100 and 98 %. The linearity of test was proved and we showed that within-run and between run precision were 5.6 and 14.66 %. Measurement of α-l-Iduronidase activity in DBS samples is an accurate test for diagnosis of MPS I and because of its rapid shipping and simplicity to keeping, DBS-based enzyme activity could be considered as a useful diagnostic tool in this disease.  相似文献   

A comparison between two different automated total 25-hydroxyvitamin D immunoassay methods using liquid chromatography-tandem mass spectrometry     

Fatma Emel Kocak  Bahadir Ozturk  Ozben Ozden Isiklar  Ozlem Genc  Ali Unlu  Irfan Altuntas 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2015,25(3):430-438

Introduction

Total 25-hydroxyvitamin D [25(OH)D] is the most reliable indicator of vitamin D status. In this study, we compared two automated immunoassay methods, the Abbott Architect 25-OH Vitamin D assay and the Roche Cobas Vitamin D total assay, with the liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Materials and methods

One hundred venous blood samples were randomly selected from routine vitamin D tests. Two of the serum aliquots were analyzed at the Abbott Architect i2000 and the Roche Cobas 6000’s module e601 in our laboratory within the same day. The other serum aliquots were analyzed at the LC-MS/MS in different laboratory. Passing-Bablok regression analysis and Bland-Altman plot were used to compare methods. Inter-rater agreement was analyzed using kappa (κ) analysis.

Results

The Roche assay showed acceptable agreement with the LC-MS/MS based on Passing-Bablok analysis (intercept: -5.23 nmol/L, 95% CI: -8.73 to 0.19; slope: 0.97, 95% CI: 0.77 to 1.15). The Abbott assay showed proportional (slope: 0.77, 95% CI: 0.67 to 0.85) and constant differences (intercept: 17.08 nmol/L; 95% CI: 12.98 to 21.39). A mean bias of 15.1% was observed for the Abbott and a mean bias of -14.1% was observed for the Roche based on the Bland-Altman plots. We found strong to nearly perfect agreement in vitamin D status between the immunoassays and LC-MS/MS. (κ: 0.83 for Abbott, κ: 0.93 for Roche) using kappa analysis.

Conclusion

Both immunoassays demonstrated acceptable performance, but the Roche Cobas assay demonstrated better performance than the Abbott Architect in the studied samples.Key words: 25-Hydroxyvitamin D, chromatography, immunoassay, methods, tandem mass spectrometry  相似文献   

Repulsion-based model for contact angle saturation in electrowetting     

Hassan Abdelmoumen Abdellah Ali  Hany Ahmed Mohamed  Mohamed Abdelgawad 《Biomicrofluidics》2015,9(1)

We introduce a new model for contact angle saturation phenomenon in electrowetting on dielectric systems. This new model attributes contact angle saturation to repulsion between trapped charges on the cap and base surfaces of the droplet in the vicinity of the three-phase contact line, which prevents these surfaces from converging during contact angle reduction. This repulsion-based saturation is similar to repulsion between charges accumulated on the surfaces of conducting droplets which causes the well known Coulombic fission and Taylor cone formation phenomena. In our model, both the droplet and dielectric coating were treated as lossy dielectric media (i.e., having finite electrical conductivities and permittivities) contrary to the more common assumption of a perfectly conducting droplet and perfectly insulating dielectric. We used theoretical analysis and numerical simulations to find actual charge distribution on droplet surface, calculate repulsion energy, and minimize energy of the total system as a function of droplet contact angle. Resulting saturation curves were in good agreement with previously reported experimental results. We used this proposed model to predict effect of changing liquid properties, such as electrical conductivity, and system parameters, such as thickness of the dielectric layer, on the saturation angle, which also matched experimental results.  相似文献   

全面反映民族生活的画卷--论新疆柯尔克孜小说集《黑脸山仙》     

张海燕  沙依甫别克·阿里 《喀什师范学院学报》2015,(1)

新时期柯尔克孜族小说集《黑脸山仙》以立足现实、反映民族生活的现实主义精神,超越民族地域界限的世界性因素,以及鲜明的民族地域特色与现代表现手段相结合的特点,展示了新时期柯尔克孜小说的成就及成长历程。  相似文献   

Preparation of carbon encapsulated iron nanoparticles with very thin shells by DC arc discharge     

崔屾  张丽爽  崔兰  张帆  林奎  靳凤民  李玲 《天津大学学报(英文版)》2015,21(1):11-18

Carbon encapsulated iron nanoparticles(CEINPs) with very thin shells and good core-shell structures were prepared by DC arc discharge at argon intake temperature(AIT) of 800 ℃. The results of high resolution transmission electron microscope(HRTEM), energy dispersive X-ray(EDX) spectroscope, X-ray diffraction(XRD), and X-ray photoelectron spectroscope(XPS) characterizations on the product B show that the thickness of the carbon shells of CEINPs in the product B is in the range of ca. 0.5—5.3 nm, i. e., which can be as thin as only two layers of graphite. The average diameter of the CEINPs is about 24. 7 nm. The total content of Fe element in the product B is 77.0 wt%. The saturation magnetization(Ms) and coercivity(Hc) of the product B are 107.4 emu/g and 143 Oe, respectively. The formation of the CEINPs in the product B is discussed briefly.  相似文献   

The Effect of Group Logotherapy on Meaning in Life and Depression Levels of Iranian Students     

Somaye Robatmili  Faramarz Sohrabi  Mohammad Ali Shahrak  Siavash Talepasand  Mostafa Nokani  Mohaddese Hasani 《International journal for the advancement of counseling》2015,37(1):54-62

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Exploring anomalies in Indigenous student engagement: findings from a national Australian survey of undergraduates     

Christine Asmar  Susan Page  Ali Radloff 《高等教育研究与发展》2015,34(1):15-29

Increases in participation by Aboriginal and Torres Strait Islander students in higher education across Australia continue to be promising. However, it is also known that Indigenous students' attrition, retention and completion rates remain areas of concern. In this paper, we report our findings from an analysis of Indigenous student responses to the 2009 Australasian Survey of Student Engagement. Overall, Indigenous Australian students express positive responses in relation to engagement, but are more likely than non-Indigenous students to be planning to depart. We explore this somewhat unexpected anomaly, whilst also suggesting that much more needs to be known about our Indigenous students, including, for example, whom they may interact with at university; where they turn for support; and why they may decide to leave. Our findings strongly indicate that better national and institutional data are needed to address the current gaps in knowledge relating to Indigenous student populations in Australia and around the world.? In this paper, the term ‘Indigenous’ refers to Australian students who are of self-declared Aboriginal and/or Torres Strait Islander background, while ‘non-Indigenous’ refers to all other Australians.  相似文献   

Hasse,Cathrine (2015) An anthropology of learning. Springer (Berlin) isbn 978‐94‐017‐9605‐7 320 pp £90 (ebook £72) http://www.springer.com/us/book/9789401796057#          下载免费PDF全文

Mohammad Ali Salmani Nodoushan 《British journal of educational technology : journal of the Council for Educational Technology》2015,46(6):E30-E31

This book combines cultural models with practice‐based learning to develop a new theory of cultural learning. Drawing on insights from anthropology, it argues how collective and social cultures can emerge from an “engaged” learning process. The book opens a new window on human learning; however, you will probably find only parts of it of some interest or use. Mohammad Ali Salmani Nodoushan  相似文献   

Incorporating AI and learning analytics to build trustworthy peer assessment systems     

Ali Darvishi  Hassan Khosravi  Shazia Sadiq  Dragan Gašević 《British journal of educational technology : journal of the Council for Educational Technology》2022,53(4):844-875

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