Inborn errors of metabolism: Review and data from a tertiary care center     

Ananth N. Rao  J. Kavitha  Minakshi Koch  V. Suresh Kumar 《Indian journal of clinical biochemistry : IJCB》2009,24(3):215-222

Inherited metabolic disorders are a heterogeneous group of genetic conditions mostly occurring in childhood. They are individually rare but collectively numerous, causing substantial morbidity and mortality. We have retrospectively reviewed a total of eight hundred and sixty nine cases with different age groups that had been referred from several diagnostic centers and hospitals of India to the Department of Metabolism in Narayana Hrudayalaya, as cases suspected with inborn errors of metabolism. Advanced techniques applied were to diagnose the disorders of inborn errors of metabolism. Data analyzed indicates occurrence of several metabolic disorders in our population. The need to screen for an inborn error of metabolism arises out of the fact that most cases take to irreversible effects as time progress. Emphasis has to be laid on early detection and prompt management, which could help in alleviating symptoms and preventing complications and consequent incapacitation.  相似文献   

Studies on the antiperoxide and antihaemolytic action of polyamines using human erythrocytes     

Chethana Chetan  Ashok Prabhu  Vivian D'Souza  Shrinivas B. Rao 《Indian journal of clinical biochemistry : IJCB》2000,15(1):17-21

Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against the peroxidative action of H₂O₂ which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H₂O₂ and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines may have independent mechanism of action.  相似文献   

Seasonal variations in markers of stress and oxidative stress in rats     

Surekha Bhat  Guruprasad Rao  K. Dilip Murthy  P. Gopalakrishna Bhat 《Indian journal of clinical biochemistry : IJCB》2008,23(2):191-194

High ambient temperature has been reported to increase oxidative stress by increasing lipid peroxidation and decreasing antioxidant defence in transition dairy cows. It is also known to cause an increase in plasma cortisol levels in goats, European hedgehog and human volunteers. High levels of glucocorticoids have been reported to decrease blood glutathione and erythrocyte superoxide dismutase activity in rats. Although institutional animal houses in research laboratories of developed countries maintain animals in air-conditioned rooms at constant temperature, the same is not true of animal houses in the developing countries especially those belonging to smaller institutions and this could affect the results of the experiments being conducted on these animals. The present research study was done to assess the effects of seasonal variations on the status of erythrocyte oxidative damage, antioxidant defence and plasma cortisol levels in adult female Wistar rats. Rats were kept in their home cages and were left in non-air-conditioned procedure rooms in two different seasons, Hot season (March-May) and Cool season (June to September). Erythrocyte Thiobarbituric acid reactive substances and plasma cortisol levels were significantly increased in rats exposed to high ambient temperature and humidity of the hot season as compared to the rats of the cool season. Erythrocyte reduced glutathione levels, erythrocyte glutathione peroxidase and superoxide dismutase activities were significantly decreased in the hot season group of rats. The results of our experiments showed that exposure of adult female Wistar rats to high ambient temperature and humidity of the hot season increases neuroendocrine stress, oxidative stress and decreases antioxidant defence in them.  相似文献   

Diagnostic strategies for subclinical hypothyroidism     

Shruti Mohanty  W. Amruthlal  G. C. Reddy  G. Kusumanjali  A. S. Kanagasabapathy  Pragna Rao 《Indian journal of clinical biochemistry : IJCB》2008,23(3):279-282

The aim of this study is to delineate laboratory diagnostic strategies for subclinical hypothyroidism in patients who are clinically symptomatic but may have a normal thyroid profile. Tri — iodothyronine (T₃), thyroxine (T₄), thyroid stimulating hormone (TSH) and anti thyroid peroxidase antibodies (anti-TPO) were estimated on fasting blood samples from 99 patients using electrochemiluminescence methods on ELECSYS 1010 (Roche). 74% of study subjects had elevated anti-TPO levels.61% patients had subclinical hypothyroidism. 45 of the 61 subclinical hypothyroid patients had elevated anti-TPO levels (73%). This is an important finding suggesting an autoimmune etiology for subclinical thyroid dysfunction with a higher risk of developing overt hypothyroidism.  相似文献   

Detection of Helicobacter pylori using nested polymerase chain reaction in gastric biopsy samples     

Divya Mahajan  Anju Jain  Varsha Singh  A. K. Jain  G. R. K. Rao  Gopal Nath 《Indian journal of clinical biochemistry : IJCB》2008,23(3):243-245

Helicobacter pylori remains a controversial organism with regards to humans, its epidemiology still unclear nearly two decades after discovery. The present study was undertaken to estimate the prevalence of the organism in the gastrointestinal tract in symptomatic and asymptomatic subjects to understand its precise natural history in India. A total of 154 specimens were a part of the study. These included gastric biopsies from peptic ulcer disease and Non ulcer dyspepsia subjects, as visualized on endoscopy, saliva and stool samples from apparently normal healthy adults. Nested polymerase chain reaction was performed using the primers Hp1, Hp2, Hp3 targeting 16S rRNA gene. A prevalence of 65.1%, 100%, 66.7%, and 73.3% respectively was observed by polymerase chain reaction. No association was observed between the H.pylori status and the disease condition of the patient.  相似文献   

Malnutrition-inflammation-atherosclerosis syndrome in Chronic Kidney disease     

Pragna Rao  G. C. Reddy  A. S. Kanagasabapathy 《Indian journal of clinical biochemistry : IJCB》2008,23(3):209-217

Chronic kidney disease is becoming a major health problem globally and in India an alarming number of about 8 million people are suffering from this disease. Patients undergoing hemodialysis have a high prevalence of protein-energy malnutrition and inflammation. As these two conditions often occur concomitantly in hemodialysis patients, they have been referred together as ‘malnutrition-inflammation-atherosclerosis syndrome’ to emphasize the important association with atherosclerotic cardiovascular disease. The three factors related to the pathophysiology in these patients are dialysis related nutrient loss, increased protein catabolism and hypoalbuminemia. Inflammation in Chronic Kidney disease is the most important factor in the genesis of several complications in renal disease. Pro-inflammatory cytokines like IL-1 and TNF-alpha play a major role in the onset of metabolic alterations in Chronic Kidney disease patients. Atherosclerosis is a very frequent complication in uremia due to the coexistence of hypertension, hyperhomocysteinemia, inflammation, malnutrition and increased oxidative stress, generation of advanced glycation end products, advanced oxidation protein products, hyperlipidemia and altered structural and functional ability of HDL. LDL-cholesterol, apolipoprotein (A), apolipoprotein (B), and Lp(a) are also associated with atherosclerosis. Studies have now provided enormous data to enable the evaluation of the severity of malnutrition-inflammation-atherosclerosis syndrome as well as effective monitoring of these patients.  相似文献   

Radioprotective effects of ocimum flavonoids on leukocyte oxidants and antioxidants in oral cancer     

K. Reshma  A. V. Rao  M. Dinesh  D. M. Vasudevan 《Indian journal of clinical biochemistry : IJCB》2008,23(2):171-175

Oxidants (NADPH oxidase and myeloperoxidase) and antioxidants (GSH,GSH peroxidase, SOD and glucose 6 phosphate dehydrogenase, that provides NADPH for antioxidants) were assayed in the neutrophils from oral cancer patients, in three stages viz, baseline samples,15 days after radiation and 30 days following radiation. These samples were obtained from 2 groups of patients. Group A that received radiation alone and Group B that received radiation and ocimum flavonoids,a radioprotector. The results showed a significant fall in the SOD levels in the second follow up of group B.Glucose 6 phosphate dehydrogenase showed significant increase only in the first follow up of patients who received Ocimum flavonoids. Except for these findings all other parameters remained statistically nonsignificant.  相似文献   

A Curve for all reasons     

T. Ramakrishna Rao 《Resonance》2000,5(4):85-90

The cause-effect relationship for a wide variety of biological processes from molecular to ecosystem levels can be described by a curvilinear function called the rectangular hyperbola. Although a simple algebraic equation adequately describes this curve, biological models have generated different equations incorporating biologically meaningful parameters. The Michelis-Menten equation describing enzyme kinetics, and the Type II functional response of predators to changing prey densities are two such examples.  相似文献   

历史视野中的澳门     

饶咬成 《郧阳师范高等专科学校学报》2000,(1)

澳门在历史上的地位是显赫的 ,它是中国历史的缩影 ,澳门问题是殖民主义的产物 ,澳门回归是中国人民完成统一大业使命的百年追求。  相似文献   

论技术类科技期刊的品牌创建——《中国南方果树》33年办刊实践与探索   总被引：7，自引：4，他引：3  

饶述军  吴涛  董朝菊  陈云  王应旭  章瑾 《编辑学报》2006,18(4):303-305

在总结中国南方果树杂志社33年办刊工作实践的基础上,从刊物定位、编读关系、刊物质量、刊物特色、刊物经营、刊物文化等6个方面论述技术类期刊的品牌创建.  相似文献