Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions     

Cuhadar S  Atay A  Koseoglu M  Dirican A  Hur A 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2012,22(2):202-214

Introduction

The collected and shipped blood samples are exposed to a various extra-analytical factors prior to analysis. The aim of the study was to determine the stability of analytes in serum gel tubes and plain tubes exposed to a range of storage temperatures and times after centrifugation.

Materials and methods:

Fifteen healthy volunteers were recruited and venous blood was collected into four tubes, two with and two without gel separator. Analyzing the baseline samples in 30 min, all were stored at 4ºC or 24ºC for 6, 12, 18, 24, 30, 36, 48 and 72 hours and 1 week. Sixteen biochemical anaytes were measured on each sample. Variations remained under the desirable bias considered as clinically insignificant.

Results:

On day three, most analytes remained stable including albumin, protein, creatinine, cholesterol, triglycerides, gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LD) regardless of tube types. Glucose concentration decreased markedly (P = 0.001) beginning from the first hours of storage in plain serum. The stability maximized for the analytes including glucose, total bilirubin, urea nitrogen (BUN), uric acid stored at 4 ºC in gel tubes. Aspartate aminotransferase (AST) activity increased significantly (P = 0.002) up to 48-h, however bias was not significant clinically. High density lipoprotein (HDL) concentration was stable in gel tubes at 24 ºC, in plain tubes at 4 ºC stored up to 36-h.

Conclusion:

Serum gel or non-gel tubes might be used interchangeably for 11 analytes chilled or at 24 ºC, whereas some restrictions must be applied for glucose, AST, BUN, HDL, and uric acid.  相似文献   

我国公共政策执行偏差问题探讨     

杨昆 《河西学院学报》2010,26(3):56-58

由于复杂的环境和各种不确定因素的影响,公共政策执行其行为效果往往会偏离政策目标。本文从公共政策执行中的偏差表现进行阐述,分析造成偏差的因素,并提出相应的解决对策,以保证公共政策执行的有效性。  相似文献   

Physical and psychosocial aspects of the learning environment in the science laboratory and their relationship to teacher satisfaction     

Che Nidzam Che Ahmad  Kamisah Osman  Lilia Halim 《Learning Environments Research》2013,16(3):367-385

This paper is a report of a survey conducted to determine teachers’ perception of the science laboratory learning environment and the relationship between different aspects of this environment and satisfaction from teaching and learning. Teachers’ perceptions of psychosocial aspects were measured by use of the Science Laboratory Environment Inventory (SLEI), whereas perceptions of physical aspects were measured by use of the Physical Science Laboratory Environment Inventory. The level of satisfaction was measured by use of a satisfaction scale (SC). Data were collected from 800 science teachers in secondary schools in Malaysia. Analysis of findings revealed that teachers have positive attitudes on all SLEI scales, with the exception of the open-ended scale. In terms of physical aspects, teachers regard lighting and technology as highly important but furniture and equipment, space, air quality and safety as of moderate importance only. Results also showed that teachers’ satisfaction from teaching in the laboratory was positive. Subsequent analysis revealed a significant predictive relationship between teachers’ perception of science laboratory learning environments and their teaching and learning satisfaction.  相似文献   

Cultural Studies in Turkey: Education and Practice     

Gönül Pultar  Ayşe Lahur Kırtunç 《Review of Education, Pedagogy & Cultural Studies》2013,35(2-3):129-153

  相似文献   

Profile and Motivations of Registered Whole-Body Donors in Turkey: Istanbul University Experience     

İlke Ali Gürses  Ahmet Ertaş  Başak Gürtekin  Osman Coşkun  Mehmet Üzel  Özcan Gayretli  Mehmet Selman Demirci 《Anatomical sciences education》2019,12(4):370-385

Little is known regarding the profiles of whole body donors in Muslim majority countries where donation is scarce. Therefore, this study aims to profile registered donors in Turkey by means of a survey. The explored data could be used to improve ongoing campaign efforts and ethical practices such as commemoration services. Registered donors of the donation programs at the two faculties of medicine of Istanbul University were compared with the national population and a cluster analysis was performed to reveal any concealed sub-groups. Data from 188 respondents were analyzed. The majority of registered donors were married (42%), male (65.4%), aged over 50 years (76%), held a tertiary education degree (49.7%), and were irreligious (58.5%). Cluster analysis revealed two groups with significantly different educational levels, marital statuses, and religious choices. Regarding whether their bodies could be used for education or research, the majority (64.5%) of the respondents left the decision to the anatomy department. Similarly, 73.8% approved indefinite use of their organs, body parts and/or skeletons. The respondents were also willing to share their medical history (94.2%) and personal information (81.6%) if needed. Motivational themes for body donation including usefulness, impermanence, religion, awareness, and kinship were devised after a thematic analysis. Among the respondents, 56.5% were registered organ donors and 63.3% were frequent blood donors. The results of this study provide data that may help revising informed consent forms, developing and implementing thanksgiving ceremonies, and selecting additional targets for supporting body donation campaign activities such as organ and blood donation units.  相似文献   

Learner-friendly textbooks: Chemistry texts based on a constructivist view of learning     

Uğur Taşdelen  Fitnat Köseoğlu 《Asia Pacific Education Review》2008,9(2):136-147

In this study, the use of inquiry methods, learning cycles, a conceptual change model and analogy in creating alternative science texts was discussed. An alternative text on the topic of acids and bases was created by integrating the methods and models discussed in this paper. The alternative text and a sample of a traditional text taken from a textbook, which is still used in Turkish high schools, were given to two groups, totaling 42 pre-service teachers—the alternative text was given to an experimental group and the traditional text to a control group—in an experimental setting and their understandings of acids and bases were compared. In addition, in the second step of the study, the pre-service teachers read both texts and indicated their preferences in terms of interest, understandability and helpfullness.  相似文献   

A Student-centred Approach: Assessing the Changes in Prospective Science Teachers’ Conceptual Understanding by Concept Mapping in a General Chemistry Laboratory     

Osman Nafiz Kaya 《Research in Science Education》2008,38(1):91-110

Although researchers in higher education propose alternatives to traditional approaches to assessment, traditional methods are commonly used in college or university science courses. The purpose of this study was to explore the feasibility and validity of Prospective Science Teachers’ (PSTs) concept maps as authentic assessment tools in a student-centred approach to describe the changes in the conceptual understanding of the PSTs in general chemistry laboratory investigations. After the PSTs (n = 47) decided on important issues, such as who would assess their concept maps and what scoring strategy and criteria would be used, they practiced assessing their own and peers’ concept maps during the first five laboratory investigations. They subsequently constructed and assessed pre- and post-laboratory concept maps in a student-centred approach consisting of self, peer, and instructor assessments for the five remaining laboratory investigations. The results of the study showed using pre- and post-laboratory concept maps as authentic assessment tools in a student-centred approach was valid and reliable for describing the conceptual understanding of the PSTs in a university general chemistry laboratory course. The results of individual interviews indicated most PSTs had positive views of their assessment practices in the laboratory course. This study also provides pedagogical implications for the training of science teachers.  相似文献   

Opening the black box: user-log analyses of children’s e-Book reading and associations with word knowledge     

Umarji  Osman  Day  Stephanie  Xu  Ying  Zargar  Elham  Yu  Renzhe  Connor  Carol 《Reading and writing》2021,34(3):627-657

Reading and Writing - Third to fifth graders read an interactive choose-your-own adventure e-Book. User logs recorded their reading behaviors and were used to investigate students’...  相似文献   

浅析管理统计在企业管理中的运用     

薛桂云 《科技与管理》2002,4(1):59-60

着重指出了管理统计在企业管理中应用的重要性，并对企业管理的发展进行了探讨，同时，结合企业管理的实际情况，阐述了管理统计在企业管理中运用的思路。  相似文献   

A Bandwidth expanding scheme for communication channels with noiseless feedback in the presence of unknown jamming noise     

Tamer Başar  Tangül Ü. Başar 《Journal of The Franklin Institute》1984,317(2):73-88

This paper treats the problem of transmitting a Gaussian discrete-time Markov process over a time-discrete additive white Gaussian channel with noiseless feedback which is subjected to statistically unknown jamming noise (satisfying a given power constraint). The channel is used more than once during the interval between the production of successive source letters, and the jamming noise is either allowed to be correlated with the encoder output, or forced to be totally independent of it. The complete solution obtained in the paper under a minimax criterion indicates that the optimum encoder-decoder structures are linear,and the least favorable jamming noise is a Gaussian process.  相似文献