Free radical injury and antioxidant status in patients with benign prostate hyperplasia and prostate cancer     

D. S. L. Srivastava  R. D. Mittal 《Indian journal of clinical biochemistry : IJCB》2005,20(2):162-165

Reactive oxygen species and other free radicals are known to be the mediators of phenotypic and genotypic changes that lead from mutation to neoplasia. There are some primary antioxidants such as glutathione peroxidase (GPx), glutathione S-transferases (GSTs) and reduced glutathione, which protect against callular and molecular damage caused by the reactive oxygen metabolites (ROMs). The present study was conducted to determine the level of malondialdehyde (MDA), as an index of lipid peroxidation, along with the GPx, GSTs activities and level of reduced glutathione in 45 prostate cancer (PC) patients, 55 benign prostate hyperplasia (BPH) patients as compared to the controls. Significant higher levels of MDA and GSTs activities in the serum, (P<0.005) and significant lower levels of reduced GSH concentration and GPx activity in blood haemolysates (P<0.05) of PC and BPH patients were observed as compared to the controls. The relatively higher GSTs activity and low level of reduced GSH may be due to the response of increased reactive oxygen metabolites production in the blood. The higher MDA and lower GPx activities may be inadequate to detoxify high levels of H₂O₂ into H₂O leading to the formation of the^*OH radical followed by MDA. This result hypothesizes that oxidant-antioxidant imbalance may be one of the major factor responsible for the development of prostate cancer and benign prostate hyperplasia.  相似文献   

Blast transformation of lymphocytes stimulated with phytohaemagglutinin in dilated cardiomyopathy (DCM) patients     

R. L. Tripathi  K. K. Talwar  K. S. S. Saibaba  L. M. Srivastava 《Indian journal of clinical biochemistry : IJCB》1996,11(2):168-170

Blast transformation of peripheral blood lymphocytes (PBL) in dilated cardiomyopathy (DCM) patients and normal controls was examined to assess the cell mediated immune response of these patients. Uptake of³H-thymidine by PBL of DCM patients incubated for 72 hrs with phytohaemagglutinin (PHA) was 17787±9513 CPM and without PHA was 1475±529 CPM showing a stimulation index (SI) by PHA of 12.95±6.35. The corresponding values in healthy controls were 55078±12282 and 1868±792 respectively with a SI of 38.0±26.0. Blast transformation of PBL by PHA was significantly suppressed in DCM patients as compared to normal controls. This suppression was found to be highly significant and possibly indicates the defective cell medicated immune response in DCM patients.  相似文献   

A biologically active constituent of withania somnifera (ashwagandha) with antistress activity     

Parvinder Kaur  Sheenu Mathur  Meenakshi Sharma  Manisha Tiwari  K. K. Srivastava  Ramesh Chandra 《Indian journal of clinical biochemistry : IJCB》2001,16(2):195-198

In Ayurvedic medicine,Withania somnifera (Ashwagandha) is well known for its anti-stress activity. A passive rat experimental model, where the animals are subjected to multiple stress of cold, hypoxia, restraint (C-H-R) has been developed to evaluate adaptogenic properties of various fractions of W. somnifera root extracts. We have carried out extraction of roots of W. somnifera with water and further isolated one of the active constituents called compound X and also tested its anti-stress activity in C-H-R model. The effect of administration of W. somnifera water suspension (360 mg/Kg bw) and compound X (20mg/Kg bw) on the fall and recovery of colonic temperature was noted. There was an increase of ≈38% and ≈54% in the time taken to attain T_rec 23°C by rats given a single dose of fresh aqueous suspension and biologically active constituent (Compound X) respectively, where as decrease in the recovery time to attain T_rec 37°C is ≈13% and ≈33% respectively, as compared to control group. It is clear that rats treated with the fresh aqueous suspension and compound X ofWithania somnifera, could withstand the multiple stress of C-H-R better than control group.  相似文献   

Insulin Resistance in Young Obese Subjects and Its Relation to Smoking (A Pilot Study)     

Aarzoo Juneja  Shridhar Dwivedi  D. K. Srivastava  Kailash Chandra 《Indian journal of clinical biochemistry : IJCB》2017,32(1):99-102

Insulin resistance is a condition in which cells fail to respond to the normal actions of insulin. Dietary fat, obesity and smoking have been attributed to increase insulin resistance. However, the prevalence of insulin resistance in young obese subjects and its relation to smoking is not well established. This study comprising seventy-five healthy young adults was undertaken to find insulin resistance in obese smokers and non smokers both. Present study showed an overall prevalence of raised homeostatic model assessment of insulin resistance in 14.7 % otherwise healthy young subjects (20–30 years age group). Non-smokers did not show any significant correlation between insulin resistance and body mass index at either stage (normal, pre-obese as well as obese). Smokers also did not show any significant difference of insulin resistance in normal and pre-obese stages. However, marked increase in homeostatic model assessment of insulin resistance was observed in obese smokers. Homeostatic model assessment of insulin resistance showed a linear trend in relation to body mass index and its values were found to be higher in smokers. Obesity combined with smoking demonstrated statistically significant increase in homeostatic model assessment of insulin resistance.  相似文献