全文获取类型
收费全文 | 269篇 |
免费 | 0篇 |
国内免费 | 2篇 |
专业分类
教育 | 236篇 |
科学研究 | 17篇 |
体育 | 11篇 |
综合类 | 3篇 |
信息传播 | 4篇 |
出版年
2023年 | 1篇 |
2021年 | 1篇 |
2020年 | 6篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 3篇 |
2012年 | 15篇 |
2011年 | 48篇 |
2010年 | 21篇 |
2009年 | 5篇 |
2008年 | 13篇 |
2007年 | 15篇 |
2006年 | 25篇 |
2005年 | 20篇 |
2004年 | 18篇 |
2003年 | 14篇 |
2002年 | 24篇 |
2001年 | 13篇 |
2000年 | 6篇 |
1999年 | 5篇 |
1997年 | 1篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有271条查询结果,搜索用时 15 毫秒
101.
嘟嘟博士 《课堂内外(小学版)》2004,(Z1)
从小到大,我们玩过很多玩具和游戏,度过了美好的童年,并学到许多有用的知识。但是,有没有同学想到过,在游戏的过程中,我们也可以完成一项项小小的发明呢?我们把游戏分为玩具和智力两类,每一种游戏里都藏着许多有意义的发明。发明玩具游戏的 女孩子都玩过跳绳,千篇一律的材料和样式,而且一到晚上就不能玩。想想圣诞节或明星演唱会上此起彼伏的荧光棒,我们是不是可以做一项小发明呢?发明之一:发光跳绳。给绳子涂抹上荧光材料,夜晚跳绳时,绳子舞成一个两头尖、中间鼓的飞艇形状,是不是既漂亮又好玩?沿着这个思路,还会有很多奇妙的产品诞生,… 相似文献
102.
Zhang Q Chen QH Fu ML Wang JL Zhang HB He GQ 《Journal of Zhejiang University. Science. B》2008,9(7):527-535
The bglS gene encoding endo-1,3-1,4-β-glucanase from Bacillus subtilis was cloned and sequenced in this study. The bglS expression cassette, including PGK1 promoter, bglS gene fused to the signal sequence of the yeast mating pheromone α-factor (MFals), and ADH1 terminator with G418-resistance as the selected marker, was constructed. Then one of the PEP4 allele of Saccharomyces cerevisiae WZ65 strain was replaced by bglS expression cassette using chromosomal integration of polymerase chain reaction (PCR)-mediated homologous recombination, and the bglS gene was expressed simultaneously. The recombinant strain S. cerevisiae (SC-βG) was preliminarily screened by the clearing hydrolysis zone formed after the barley β-glucan was hydrolyzed in the plate and no proteinase A (PrA) activity was measured in fermenting liquor. The results of PCR analysis ofgenome DNA showed that one of the PEP4 allele had been replaced and bglS gene had been inserted into the locus of PEP4 gene in recombinant strains. Different endo-1,3-1,4-β-glucanase assay methods showed that the recombinant strain SC-βG had high endo-1,3-1,4-β-glucanase expression level with the maximum of 69.3 U/(h-ml) after 60 h of incubation. Meanwhile, the Congo Red method was suitable for the determination of endo-1,3-1,4-β-glucanase activity during the actual brewing process. The current research implies that the constructed yeast strain could be utilized to improve the industrial brewing property of beer. 相似文献
103.
女人谁不希望自己拥有一个健美窈窕的体形?所以怀孕和生产过程 令她们最担心的是怕产后会失去女性风姿,变得体态肥胖,臃肿难看。的确,绝大多数女性在未孕前身材苗条,乳房丰满,腰细如柳,十分富有曲线美,而产后很可能变得乳房松弛下坠,腹部隆起下垂,腰部粗圆,臀部宽大,一时难以恢复孕前状态。 相似文献
104.
105.
Di JZ Han XD Gu WY Wang Y Zheng Q Zhang P Wu HM Zhu ZZ 《Journal of Zhejiang University. Science. B》2011,12(10):805-811
Global DNA hypomethylation has been associated with increased risk for cancers of the colorectum, bladder, breast, head and
neck, and testicular germ cells. The aim of this study was to examine whether global hypomethylation in blood leukocyte DNA
is associated with the risk of hepatocellular carcinoma (HCC). A total of 315 HCC cases and 356 age-, sex- and HBsAg status-matched
controls were included. Global methylation in blood leukocyte DNA was estimated by analyzing long interspersed element-1 (LINE-1)
repeats using bisulfite-polymerase chain reaction (PCR) and pyrosequencing. We observed that the median methylation level
in HCC cases (percentage of 5-methylcytosine (5mC)=77.7%) was significantly lower than that in controls (79.5% 5mC) (P=0.004, Wilcoxon rank-sum test). The odds ratios (ORs) of HCC for individuals in the third, second, and first (lowest) quartiles
of LINE-1 methylation were 1.1 (95% confidence interval (CI) 0.7–1.8), 1.4 (95% CI 0.8–2.2), and 2.6 (95% CI 1.7–4.1) (P for trend <0.001), respectively, compared to individuals in the fourth (highest) quartile. A 1.9-fold (95% CI 1.4–2.6) increased
risk of HCC was observed among individuals with LINE-1 methylation below the median compared to individuals with higher (>median)
LINE-1 methylation. Our results demonstrate for the first time that individuals with global hypomethylation measured in LINE-1
repeats in blood leukocyte DNA have an increased risk for HCC. Our data provide the evidence that global hypomethylation detected
in the easily obtainable DNA source of blood leukocytes may help identify individuals at risk of HCC. 相似文献
106.
107.
The anatomy curriculum at Namibia's first, and currently only, medical school is clinically oriented, outcome-based, and includes all of the components of modern anatomical sciences i.e., histology, embryology, neuroanatomy, gross, and clinical anatomy. The design of the facilities and the equipment incorporated into these facilities were directed toward simplification of work flow and ease of use by faculty, staff, and students. From the onset, the integration of state of the art technology was pursued to facilitate teaching and promote a student-centered pedagogical approach to dissections. The program, as realized, is comprised of three 16-week semesters with seven hours of contact time per week, namely three hours of lectures and four hours of dissection laboratory and microscopy time. Set outcomes were established, each revolving around clinical cases with integrated medical imaging. The design of the facility itself was not constrained by a legacy structure, allowing the School of Medicine, in collaboration with architects and contractors, to design the building from scratch. A design was implemented that allows for the sequential processing of cadaveric material in a unidirectional flow from reception, to preparation, embalming, storage, dissection, and maceration. Importantly, the odor of formaldehyde typically associated with anatomy facilities was eliminated outside of the dissection areas and minimized within via a high-performance ventilation system. By holistically incorporating an integrated curriculum, facility design, and teaching at an early stage, the authors believe they have created a system that might serve as a model for new anatomy programs. 相似文献
108.
109.
110.
小朋友们一定不会相信,计算机有时也会生病。计算机要是得病的话,它内部储(chu)存的数据就会遭(zao)到破坏,运行速度会减慢,严重的还会让整个系统瘫痪(tan huan)呢,造成的经济损失非常巨大。这些都是因为——“病毒”。 相似文献