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In healthcare practice, the sedimentation rate of red blood cells (erythrocytes) is a widely used clinical parameter for screening of several ailments such as stroke, infectious diseases, and malignancy. In a traditional pathological setting, the total time taken for evaluating this parameter varies typically from 1 to 2 h. Furthermore, the volume of human blood to be drawn for each test, following a gold standard laboratory technique (alternatively known as the Westergren method), varies from 4 to 5 ml. Circumventing the above constraints, here we propose a rapid (∼1 min) and highly energy efficient method for the simultaneous determination of hematocrit and erythrocyte sedimentation rate (ESR) on a microfluidic chip, deploying electrically driven spreading of a tiny drop of blood sample (∼8 μl). Our unique approach estimates these parameters by correlating the same with the time taken by the droplet to spread over a given radius, reproducing the results from more elaborate laboratory settings to a satisfactory extent. Our novel methodology is equally applicable for determining higher ranges of ESR such as high concentration of bilirubin and samples corresponding to patients with anemia and patients with some severe inflammation. Furthermore, the minimal fabrication steps involved in the process, along with the rapidity and inexpensiveness of the test, render the suitability of the strategy in extreme point-of-care settings. 相似文献
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Rajarshi Sarkar 《Indian journal of clinical biochemistry : IJCB》2014,29(2):189-195
Although 3rd generation TSH assays are the most widely used immunoassays, credible comparison studies, specially involving Indian sub-populations are practically non-existent. To compare the TSH measurements between chemiluminescence (Architect) and electrochemiluminescence (Cobas) inmmunoassays in an urban ambulatory Indian population. 1,615 subjects were selected randomly from the usual laboratory workflow, their TSH measured in Architect and Cobas and the paired data thus generated were statistically analysed. TSH values of Cobas were observed to be higher than the Architect values by 28.7 %, with a significant proportional difference between the two, but majority of the Cobas values (above 90 %) were within the limits of agreement with Architect values. In situations where both the instruments are in use simultaneously, a standardization of the methods is imperative, in larger interest of the patient populace. 相似文献
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In this article, we discuss at length a combinatorial problem which has been of historic interest. It has appeared as a puzzle in several different versions with varying degrees of difficulty. It can be simply stated as follows: We are given a number of coins which are otherwise identical except that there may be at most one fake coin among them which is either slightly heavier or slightly lighter than the other genuine coins. Using only a two-pan weighing balance, we must devise a weighing scheme to identify the counterfeit coin and determine whether it is heavier or lighter (or declare that all coins are normal). We construct both sequential and non-sequential (that is, simultaneously declared) weighing plans for any given number of coins containing at most one fake coin using the minimum number of weighings needed. 相似文献
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Sarama Saha Chandan Sarkar Subhash Chandra Biswas R. Karim 《Indian journal of clinical biochemistry : IJCB》2008,23(3):262-266
Abnormal lipid profile is often found in women with Polycystic Ovary Syndrome. To assess the impact of abnormal lipid profile
on atherosclerosis in young Polycystic Ovary Syndrome women, carotid intima-media thickness as judged by B-mode ultrasonography
were done in 30 young (18–35 yrs) Polycystic Ovary Syndrome women and in similarly age-matched 30 apparently healthy controls.
Compared to controls, young Polycystic Ovary Syndrome women had significantly elevated serum total cholesterol, triglyceride
and LDL-C levels and carotid intima-media thickness. HDL-C level did not differ significantly between two groups of women.
In Polycystic Ovary Syndrome women carotid intima-media thickness was positively correlated with serum total cholesterol,
triglyceride and LDL-C and negatively correlated with serum HDL-C. Our study suggests that even young Polycystic Ovary Syndrome
women are prone to atherosclerosis from early age. 相似文献
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Targeted yield concept and a framework of fertilizer recommendation in irrigated rice domains of subtropical India 总被引：1，自引：0，他引：1
Bera R Seal A Bhattacharyya P Das TH Sarkar D Kangjoo K 《Journal of Zhejiang University. Science. B》2006,7(12):963-968
Soil test crop response （STCR） correlation studies were carried out in Vindhyan alluvial plain during 2001 to 2004 taking IR-36 as test crop to quantify rice production in the context of the variability of soil properties and use of balanced fertilizers based on targeted yield concept. The soils were developed on gently sloping alluvial plain with different physiographic settings and notable variation in drainage condition. Soil properties show moderate variation in texture （loamy to clay）, organic carbon content （4.4 to 9.8 g/kg）, cation exchange capacity （10,2 to 22.4 cmol （p＋）/kg） and pH （5.3 to 6,4）, Soil fertility status for N is low to medium （224 to 348 kg/ha）, P is medium to high （87 to 320 kg/ha） and K ranges from medium to high （158 to 678 kg/ha）. Database regarding nutrient requirement in kg/t of grain produce （NR）, the percent contribution from the soil available nutrients [CS （%）] and the percent contribution from the applied fertilizer nutrients [CF （%）] were computed for calibrating and fbrmulating fertilizer recommendations. Validity of the yield target for 7 and 8 t/ha was tested in farmers＇ fields and yields targets varied at less than 10%. The percent achievement of targets aimed at different level was more than 90%, indicating soil test based fertilizer recommendation approach was economically viable within the agro-ecological zone with relatively uniform cropping practices and socio-economic conditions. 相似文献