MPG1基因驱动GFP基因在大丽轮枝菌中表达     

张斯  柳燕  徐婷婷  吕乐  竺锡武  陈集双 《科技通报》2015,(5)

为了探讨以MPG1基因作为启动子能否驱动GFP基因在大丽轮枝菌中表达,采用根癌农杆菌介导的真菌遗传转化方法,将含有MPG1-eGFP表达盒的pHMG载体转化入棉花大丽轮枝菌T-9中。经抗生素筛选后转化子约80个/106孢子,荧光显微镜观察转化子菌株孢子和菌丝在荧光显微镜下均发出绿色荧光,基因组DNA-PCR检测、GFP蛋白westernblot检测表明,GFP蛋白在大丽轮枝菌转化子中成功表达。结果表明,MPG1基因作为启动子成功驱动GFP基因在大丽轮枝菌中转录表达。  相似文献   

110kV榭烯北中线防雷研究     

崔乐 《商洛学院学报》2015,(4)

以110 k V榭烯北中线为研究对象,根据线路布置的地形地貌情况、历年落雷及事故统计,并结合线路接地电阻测量值,采用ATP软件对雷击输电线路杆塔和雷绕击输电线路的耐雷水平进行了计算。根据计算结果提出防雷方案,仿真评估了防雷方案的效果,验证了该方案的可行性,可作为该地区雷电过电压防护措施制定和实施的依据。  相似文献   

浙江省城市化与碳排放关系研究     

蔡乐  李浩 《科技与管理》2015,17(2):19-24

浙江省正处于城市化的快速发展阶段,高耗能产业快速发展,能源消费增长迅速,导致浙江省碳排放量逐年增加。在计算和分析浙江省2000—2012年城市化水平与碳排放量的基础上,运用EVIEWS7.0计量分析软件,实证检验了浙江省城市化与碳排放之间的协整关系,以及浙江省城市化发展对碳排放的长期弹性和短期弹性,同时还探究了这两者之间的因果关系。最后根据实证结果进行了综合总结并提出了有关降低浙江省碳排放的建议。  相似文献   

Proteolysis of Sardine (Sardina pilchardus) and Anchovy (Stolephorus commersonii) by Commercial Enzymes in?Saline Solutions     

Chau Minh Le  Claire Donnay-Moreno  Sandrine Bruzac  Régis Baron  Huong Thi My Nguyen  Jean Pascal Bergé 《Food Technology and Biotechnology》2015,53(1):87-90

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The citation evolution law of papers published in the same year but different month          下载免费PDF全文

GAI Shuang‐Shuang  LIU Xue‐Li  ZHANG Shi‐Le  LIU Rui‐Yuan 《Learned Publishing》2015,28(2):132-139

To explore the citation evolution of papers published in the same year but different month, we selected papers from a discipline (physical geography), a subject (diabetes: endocrine and metabolism) and a journal (Journal of Biological Chemistry) published in 2005 as research objects. These papers were divided into six groups according to the difference in publication month, and we analyzed citations to these papers for the 9 years after publication. The results showed that within 5 years after papers from physical geography were published, the overall differences in citations of papers in different groups were statistically significant (P < 0.05); after that, the differences were not statistically significant. Within 5 years after papers from diabetes (endocrine and metabolism) were published, the overall differences in citations of papers in different groups were statistically significant (P < 0.05); thereafter, the differences were not statistically significant. Within 7 years after papers from the Journal of Biological Chemistry were published, the overall differences in citations of papers in different groups were statistically significant (P < 0.05); thereafter, the differences were not statistically significant. Citations of papers followed the same pattern irrespective of discipline, subject or journal: citations of papers published in the same year but different month were obviously different in the first few publishing years, but as time went on, only the difference in publication month in a calendar year did not affect the papers' longer‐term citation.  相似文献   

Biofunctionalized nanoslits for wash-free and spatially resolved real-time sensing with full target capture     

Thierry Le?chlé  Chia-Fu Chou 《Biomicrofluidics》2015,9(3)

We propose biofunctionalized nanofluidic slits (nanoslits) as an effective platform for real-time fluorescence-based biosensing in a reaction-limited regime with optimized target capture efficiency. This is achieved by the drastic reduction of the diffusion length, thereby a boosted collision frequency between the target analytes and the sensor, and the size reduction of the sensing element down to the channel height comparable to the depletion layer caused by the reaction. Hybridization experiments conducted in DNA-functionalized nanoslits demonstrate the analyte depletion and the wash-free detection ∼10 times faster compared to the best microfluidic sensing platforms. The signal to background fluorescence ratio is drastically increased at lower target concentrations, in favor of low-copy number analyte analysis. Experimental and simulation results further show that biofunctionalized nanoslits provide a simple means to study reaction kinetics at the single-pixel level using conventional fluorescence microscopy with reduced optical depth.  相似文献   

我国台湾地区首座法学文献信息中心的创建历程——“台湾高等法院”日据时期法律文献撷英     

许乐 《高校图书馆工作》2015,(3)

作为具有重要历史价值的珍贵法律文献,“台湾高等法院”日据时期的法律文献经过台湾大学师生的分类整理,并根据五条编目的原则进行加工之后,按照语种,主要分为中文文献、日文文献、西文文献;按照载体形式不同,分为图书和期刊.由于在较长的历史时期内,这些文献的保存条件较差,所以应当加强这批文献的保护与利用,并充分发掘这些史料的学术价值.  相似文献   

灰树花液体培养基配方的研究     

马忠友  刘乐  吴萍  王松华  邓盾 《安徽科技学院学报》2011,25(3):13-17

通过正交交互设计试验优化灰树花的液体培养基配方,确定玉米粉和葡萄糖有交互作用,其比例为3∶2时为最优搭配。获得灰树花液体培养的最优配方为黄豆粉2%,玉米粉3%,葡萄糖2%,磷酸二氢钾0.05%,硫酸镁0.2%,豆油0.05%。将灰树花在50L发酵罐中进行深层培养,转速140r/min和通气量为300L/min,菌丝体产量为14.69g/L。  相似文献   

天津软土地区十字板测试的几点应用     

康乐  刘肇璞  陈志琦 《廊坊师范学院学报(自然科学版)》2011,11(2):51-52,57

收集了天津开发区、天津港、临港工业区、大港区等距海岸较近的区域及保税区空港物流加工区、东丽区滨海国际机场等距海岸较远的区域十字板原位测试资料,结合软土地区实际工程,用十字板来确定一些岩土参数,给以后的工作加以指导和借鉴。  相似文献   

亚洲一流大学本科课程设置与课程管理特点评析     

乐毅 《中国高教研究》2015,(2):46-53

亚洲一流大学都十分注重本科课程设置的顶层整体设计及课程结构的调整和优化;重视人才培养模式变革,以课程为载体,大力推行以基本素养和全人教育为目标的通识教育。设置专门的组织管理机构,强化课程管理与教学质量监督是亚洲一流大学保障其课程实施有效性的重要方式,而重视课程与教学评价的改进,强化学分制管理,倡导教学方式变革,是其课程与教学管理制度的共同经验。我国大陆研究型大学在实施本科教育改革时,可研究借鉴亚洲其他国家一流大学以及我国港台名校的课程设置模式与管理经验,以加快内涵发展与改革创建步伐。  相似文献