Microfluidic-driven viral infection on cell cultures: Theoretical and experimental study     

Elisa Cimetta  Mauro Franzoso  Marta Trevisan  Elena Serena  Alessandro Zambon  Stefano Giulitti  Luisa Barzon  Nicola Elvassore 《Biomicrofluidics》2012,6(2):024127-12

Advanced cell culture systems creating a controlled and predictable microenvironment together with computational modeling may be useful tools to optimize the efficiency of cell infections. In this paper, we will present a phenomenological study of a virus-host infection system, and the development of a multilayered microfluidic platform used to accurately tune the virus delivery from a diffusive-limited regime to a convective-dominated regime. Mathematical models predicted the convective-diffusive regimes developed within the system itself and determined the dominating mass transport phenomena. Adenoviral vectors carrying the enhanced green fluorescent protein (EGFP) transgene were used at different multiplicities of infection (MOI) to infect multiple cell types, both in standard static and in perfused conditions. Our results validate the mathematical models and demonstrate how the infection processes through perfusion via microfluidic platform led to an enhancement of adenoviral infection efficiency even at low MOIs. This was particularly evident at the longer time points, since the establishment of steady-state condition guaranteed a constant viral concentration close to cells, thus strengthening the efficiency of infection. Finally, we introduced the concept of effective MOI, a more appropriate variable for microfluidic infections that considers the number of adenoviruses in solution per cell at a certain time.  相似文献   

Equity in New Zealand university graduate outcomes: Māori and Pacific graduates     

Reremoana Theodore  Mele Taumoepeau  Jesse Kokaua  Karen Tustin  Megan Gollop  Nicola Taylor 《高等教育研究与发展》2018,37(1):206-221

Higher education confers significant private and social benefits. Māori and Pacific peoples are under-represented within New Zealand universities and have poorer labour market outcomes (e.g., lower wages, under-represented in skilled professions). A New Zealand tertiary education priority is to boost Māori and Pacific success in an effort to improve outcomes for these graduates, their communities and society in general. Using information collected in the Graduate Longitudinal Study New Zealand, we compared Māori and Pacific university graduate outcomes with outcomes of other New Zealand graduates. Data were collected when the participants were in their final year of study (n?=?8719) and two years post-graduation (n?=?6104). Employment outcomes were comparable between Māori, Pacific and other New Zealand graduates at two years post-graduation; however, Māori and Pacific graduates had significantly higher student debt burden and financial strain over time. They were significantly more likely to help others (e.g., family) across a range of situations (e.g., lending money), and reported higher levels of volunteerism compared to their counterparts. Boosting higher education success for Māori and Pacific students has the potential to reduce ethnic inequalities in New Zealand labour market outcomes and may result in significant private benefits for these graduates and social benefits as a result of their contribution to society.  相似文献   

Task avoidance, number skills and parental learning difficulties as predictors of poor response to instruction     

Niemi P  Nurmi JE  Lyyra AL  Lerkkanen MK  Lepola J  Poskiparta E  Poikkeus AM 《Journal of learning disabilities》2011,44(5):459-471

Altogether 1,285 Finnish children were followed up from the end of kindergarten through Grade 1. All were nonreaders at school entrance. The aim was to delineate predictors of resistance to treatment that are evidenced as little or no reading progress during Grade 1. On the basis of reading achievement in Grade 1 spring, four subgroups were formed. These were fast, average, and slow reading acquisition and slow progress in both reading and math. Kindergarten spring scores in phonological awareness, letter knowledge, rapid naming, and number skills differentiated well among the groups, the latter two being more robust predictors. Task avoidance added to the prediction over and above cognitive skills. Its effect disappeared when parental history of reading and math difficulties was included in the equation. The present results depict poor response to instruction as a general learning problem rather than a specific reading difficulty. Poor response to instruction differs from dyslexia also in that treatment resisters start school with cognitive prerequisites that do not indicate severe reading and math problems.  相似文献   

Prevalence and correlates of multiple victimization in a nation-wide adolescent sample     

Romano E  Bell T  Billette JM 《Child abuse & neglect》2011,35(7):468-479

Objectives

Adolescents often experience different types of victimization across a specified period of time in different situations. These multiple victimization experiences can have a number of deleterious effects on psychosocial well-being. To expand on research gathered primarily from US samples, the current study estimated the prevalence of multiple victimization in a nationally representative sample of Canadian adolescents. We also expanded on past research by adopting an ecological approach to identify correlates of multiple victimization.

Methods

Cross-sectional data from the 2000-2001 cycle of the National Longitudinal Survey of Children and Youth (NLSCY) were used to estimate the prevalence of multiple victimization (verbal harassment, threat of and actual physical assault, school social exclusion, discrimination) in 1,036 13-16 year olds. We also examined household (e.g., parental education), family (e.g., parenting practices), and adolescent (e.g., friendship quality) correlates of multiple victimization for the whole sample and separately by sex.

Results

Among the 6 in 10 adolescents who reported at least 1 victimization experience, 30.5% reported 2 types of victimization whereas 23.7% reported 3 or more types. There was an increased probability of multiple victimization (2 or more types) in adolescents who reported greater parental rejection, who engaged in more frequent out-of-school activities, and who experienced non-victimization adversity. The probability decreased if adolescents reported greater friendship quality.

Conclusions

The clustering of different types of victimization is common among adolescents. For both males and females, a difficult parent-child relationship characterized as rejecting is important when considering risk for multiple victimization, as is the adolescent's functioning outside of the home in the context of friendship quality and involvement in out-of-school activities. Non-victimization adversity (e.g., death of a loved one) also emerged as a significant multiple victimization correlate.

Practice implications

Non-physically invasive types of victimization (although adolescents also endorsed physical assault) are a reality for a number of adolescents. As such, we need to inquire about such experiences as school social exclusion, discrimination, and verbal threats in applied contexts. Moreover, in order to better identify adolescents who may be vulnerable to multiple forms of victimization, we need to adopt an ecological approach that considers individual, family, and household functioning.  相似文献   

A large-scale evaluation of the KiVa antibullying program: grades 4-6     

Kärnä A  Voeten M  Little TD  Poskiparta E  Kaljonen A  Salmivalli C 《Child development》2011,82(1):311-330

This study demonstrates the effectiveness of the KiVa antibullying program using a large sample of 8,237 youth from Grades 4-6 (10-12 years). Altogether, 78 schools were randomly assigned to intervention (39 schools, 4,207 students) and control conditions (39 schools, 4,030 students). Multilevel regression analyses revealed that after 9 months of implementation, the intervention had consistent beneficial effects on 7 of the 11 dependent variables, including self- and peer-reported victimization and self-reported bullying. The results indicate that the KiVa program is effective in reducing school bullying and victimization in Grades 4-6. Despite some evidence against school-based interventions, the results suggest that well-conceived school-based programs can reduce victimization.  相似文献   

Engaging Participative Communities in Cultural Heritage: Using Digital Storytelling in Sicily (Italy)     

Elisa Bonacini 《International Information and Library Review》2019,51(1):42-50

The paper describes the project #iziTRAVELSicilia which is using digital storytelling for engaging heritage communities. The objective of the project was to bridge the Sicilian gap in cultural heritage communication using a participatory approach with the local communities, in the framework defined by the Faro Convention. The paper evidences the digital storytelling role in engaging communities to promote their heritage, promoting new partnership with local cultural heritage institutions. The positive impact of digital storytelling in cultural heritage promotion in Sicily is evidenced by the quantitative results achieved and by the feedback collected by a survey.  相似文献   

The influence of the family educational scenario on internet use of primary and secondary school children / La influencia del escenario educativo familiar en el uso de internet en los niños de primaria y secundaria     

Sonia Padilla  Elisa Rodríguez  Míriam Álvarez  Alezandra Torres  Arminda Suárez  María-José Rodrigo 《Infancia y Aprendizaje》2015,38(2):402-434

Abstract

With the aim of studying the impact of the internet in the home, this paper analyses how the physical component (where and with whom) and attitudinal component (who decides what the child is to see on the internet and motivations for internet use) of the family educational scenario influence the frequency of internet use and diversity of uses, as reported by parents. The participants included 711 parents of children in primary and secondary school. Results showed that being in secondary school, using the internet in privacy at home, without company, internet content under the child’s control, and parental motivations based on the internet being used as a means for the child to relax, predicted higher frequency of internet use. Some of these factors, as well as the presence of peers and new parental attitudes related to the use of the internet for social and learning purposes, predicted a greater variety of uses, suggesting a more appropriate family scenario. The practical implications for parental education on this matter are described.  相似文献   

Placement,inclusion, law and teachers’ perceptions in Macao’s schools     

Vitor Teixeira  Ana Correia  Elisa Monteiro  Angus C.H. Kuok  Chris Forlin 《International Journal of Inclusive Education》2018,22(9):1014-1032

In Macao, the government has initiated a debate regarding revisions in the Decree Law in order to promote a more inclusive schooling system. In this Special Administrative Region of China, inclusive education is one of three possible types of special education that are likely be legislated in the future. The way the teachers perceive the different aspects related to inclusive education, namely the principles, concepts and law, is essential for its full implementation. The aim of this study is to understand teachers’ perceptions about the proposed amendments in the consultation document for changes in the special education regime. In particular, we focus on teachers’ acceptance of the recommended role of parents, the proposed placement models and expectations for teacher training. A mixed-methods approach with a survey of a sample of 500 teachers in private schools and interviews to a sub-sample of 20 provide the data. While agreeing in principle with the majority of the proposed changes in the Decree Law, there were several reservations made by the teachers, especially regarding the extent of parental choice, placement decisions and teacher training. Recommendations to continue the pathway for a more inclusive education system in Macao and for further research are made.  相似文献   

Book reviews     

Barbara Seels  Elisa J. Slee  Prachee Mukherjee  Gary R. Morrison 《Educational technology research and development : ETR & D》1991,39(2):89-96

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The Use of Online Support Groups to Seek Information about Chronic Illness: Applying the Theory of Motivated Information Management     

Elisa Kanter  Sam M. Dorros 《Communication quarterly》2019,67(1):100-121

The growing number of adults living with chronic illness, combined with the increased availability of illness-specific online support groups, is changing the way individuals seek information about their chronic illnesses. This study utilized elements of the theory of motivated information management (TMIM) to examine how individuals decide to seek information about their chronic illness from online support groups. Adults with chronic illness who had visited at least one online support group in the last six months completed an online survey. Structural equation model findings revealed that, although uncertainty discrepancy about a chronic illness did not predict negative emotional response, both target efficacy and communication efficacy were positively related to information-seeking. Findings aid in explaining specific processes individuals go through when deciding to visit online support groups to gather information about their chronic illnesse, but cannot conclusively support TMIM in this context.  相似文献