Life under flow: A novel microfluidic device for the assessment of anti-biofilm technologies     

Maria Salta  Lorenzo Capretto  Dario Carugo  Julian A. Wharton  Keith R. Stokes 《Biomicrofluidics》2013,7(6)

In the current study, we have developed and fabricated a novel lab-on-a-chip device for the investigation of biofilm responses, such as attachment kinetics and initial biofilm formation, to different hydrodynamic conditions. The microfluidic flow channels are designed using computational fluid dynamic simulations so as to have a pre-defined, homogeneous wall shear stress in the channels, ranging from 0.03 to 4.30 Pa, which are relevant to in-service conditions on a ship hull, as well as other man-made marine platforms. Temporal variations of biofilm formation in the microfluidic device were assessed using time-lapse microscopy, nucleic acid staining, and confocal laser scanning microscopy (CLSM). Differences in attachment kinetics were observed with increasing shear stress, i.e., with increasing shear stress there appeared to be a delay in bacterial attachment, i.e., at 55, 120, 150, and 155 min for 0.03, 0.60, 2.15, and 4.30 Pa, respectively. CLSM confirmed marked variations in colony architecture, i.e.,: (i) lower shear stresses resulted in biofilms with distinctive morphologies mainly characterised by mushroom-like structures, interstitial channels, and internal voids, and (ii) for the higher shear stresses compact clusters with large interspaces between them were formed. The key advantage of the developed microfluidic device is the combination of three architectural features in one device, i.e., an open-system design, channel replication, and multiple fully developed shear stresses.  相似文献   

Using differential ratings of perceived exertion to assess agreement between coach and player perceptions of soccer training intensity: An exploratory investigation     

Tom W. Macpherson  Shaun J. McLaren  Warren Gregson  Lorenzo Lolli  Barry Drust  Matthew Weston 《Journal of sports sciences》2013,31(24):2783-2788

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A Mössbauer study of some coloured marbles (cipollino mandolato, rosso antico and fior di pesco): implications on the nature of their colour     

Sandro Calogero  Mariangela Bertelle  Fabrizio Antonelli  Lorenzo Lazzarini 《Journal of Cultural Heritage》2000,1(4):429

⁵⁷Fe Mössbauer spectroscopy permits an explanation of the formation of the iron-bearing species present in three types of coloured marbles, known as cipollino mandolato, rosso antico and fior di pesco. Structural Fe²⁺ and Fe³⁺ located in silicates, together with the remains of weakly ferromagnetic hematite, arise from primary sediments. Fe²⁺ located in carbonates and anti-ferromagnetic hematite are formed by redox processes during marble formation. The relative contents of the iron species in the three types of marbles depend on the composition of the primary sediments and on local conditions during metamorphism. Knowledge of the type and amount of the iron species in marbles may help in the reconstruction of the redox processes, which took place during marble formation. The deviations of the Morin transition of the hematites play the role of genetic indicator because purity, crystallinity and the red hues of hematites in marbles can be related to the environment of formation.  相似文献   

The use of augmented reality to improve the development of activities of daily living in students with ASD     

Lledó  Gonzalo Lorenzo  Lledó  Asunción  Gilabert-Cerdá  Alba  Lorenzo-Lledó  Alejandro 《Education and Information Technologies》2022,27(4):4865-4885

Education and Information Technologies - In today's society, technology plays a fundamental role. More and more activities of daily life are being developed with the help of technology. One of...  相似文献