Immunoisolated Transplantation of Purified Langerhans Islet Cells in Testis Cortex of Male Rats for Treatment of Streptozotocin Induced Diabetes Mellitus     

Ali Farhangi  Dariush Norouzian  Mohammad Reza Mehrabi  Mohsen Chiani  Zahra Saffari  Maryam Farahnak  Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2014,29(4):406-417

The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food, volume of water, urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60 mg/kg dose of streptozotocin in 250–300 g (75–90 days) adult Wistar rats makes pancreas swell and causes degeneration in Langerhans islet β-cells and induces experimental diabetes mellitus in 2–4 days. For a microscopic study of degeneration of Langerhans islet β-cells of diabetic rats, biopsy from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. In this process, after collagenase digestion of pancreas, islets were isolated, dissociated and identified by dithizone method and then with enzymatic procedure by DNase and trypsin, the islet cells changed into single cells and β-cells were identified by immune fluorescence method and then assayed by flow-cytometer. Donor tissue in each step of work was prepared from 38 adult male Wistar rats weighted 250–300 g (75–90 days). Transplantation was performed in rats after 2–4 weeks of diabetes induction. In this study, the levels of insulin, C-peptide and glucose in diabetic rats reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells. Transplantation was performed under the cortex of testis as immunoisolated place for islet cells transplantation.  相似文献   

Measurement and control of pressure driven flows in microfluidic devices using an optofluidic flow sensor     

Mohammad Sadegh Cheri  Hamidreza Shahraki  Jalal Sadeghi  Mohammadreza Salehi Moghaddam  Hamid Latifi 《Biomicrofluidics》2014,8(5)

Measurement and control of pressure-driven flow (PDF) has a great potential to enhance the performance of chemical and biological experiments in Lab on a Chip technology. In this paper, we present an optofluidic flow sensor for real-time measurement and control of PDF. The optofluidic flow sensor consists of an on-chip micro Venturi and two optical Fabry-Pérot (FP) interferometers. Flow rate was measured from the fringe shift of FP interferometers resulted from movement fluid in the on-chip micro Venturi. The experimental results show that the optofluidic flow sensor has a minimum detectable flow change of 5 nl/min that is suitable for real time monitoring and control of fluids in many chemical and biological experiments. A Finite Element Method is used to solve the three dimensional (3D) Navier–Stokes and continuity equations to validate the experimental results.  相似文献   

Exact solutions for the generalized KdV equation by using Backlund transformations     

Hassan A. Zedan 《Journal of The Franklin Institute》2011,348(8):1751-1768

In this paper, the Backlund transformations for the generalized KdV equation are constructed through Ablowitz–Kaup–Newell–Segur (AKNS) system in using Ricatti's form of the inverse method. The derived Backlund transformations are used to generate new classes of exact solutions. The technique developed here is based on the construction of wave functions which are the solutions of the associated AKNS. From known simple solutions we shall construct other solitons and wave solutions as well.  相似文献   

Feedback after good versus poor trials affects intrinsic motivation     

Badami R  VaezMousavi M  Wulf G  Namazizadeh M 《Research quarterly for exercise and sport》2011,82(2):360-364

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The Master’s Program in Computer-Mediated Computer Communications: A Comparative Study of Two Cohorts of Students     

Mohammad Issack Santally  Jerome Raverdy 《Educational technology research and development : ETR & D》2006,54(3):312-326

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Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq     

Al-Bayati FA  Al-Mola HF 《Journal of Zhejiang University. Science. B》2008,9(2):154-159

Antimicrobial activity of organic and aqueous extracts from fruits, leaves and roots of Tribulus terrestris L., an Iraqi medicinal plant used as urinary anti-infective in folk medicine, was examined against 11 species of pathogenic and non-pathogenic microorganisms: Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Corynebacterium diphtheriae, Escherichia coli,Proteus vulgaris, Serratia marcescens, Salmonella typhimurium, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans using microdilution method in 96 multiwell microtiter plates. All the extracts from the different parts of the plant showed antimicrobial activity against most tested microorganisms. The most active extract against both Gram-negative and Gram-positive bacteria was ethanol extract from the fruits with a minimal inhibitory concentration (MIC) value of 0.15 mg/ml against B. subtilis,B. cereus, P. vulgaris and C. diphtheriae. In addition, the same extract from the same plant part demonstrated the strongest antifungal activity against C. albicans with an MIC value of 0.15 mg/ml.  相似文献   

Antagonistic interaction between <Emphasis Type="Italic">Trichoderma asperellum</Emphasis> and <Emphasis Type="Italic">Phytophthora capsici</Emphasis> in vitro     

Heng Jiang  Liang Zhang  Jing-ze Zhang  Mohammad Reza Ojaghian  Kevin D. Hyde 《Journal of Zhejiang University. Science. B》2016,17(4):271-281

Phytophthora capsici is a phytopathogen that causes a destructive pepper blight that is extremely difficult to control. Using a fungicide application against the disease is costly and relatively ineffective and there is also a huge environmental concern about the use of such chemicals. The genus Trichoderma has been known to have a potential biocontrol issue. In this paper we investigate the mechanism for causing the infection of T. asperellum against P. capsici. Trichoderma sp. (isolate CGMCC 6422) was developed to have a strong antagonistic action against hyphae of P. capsici through screening tests. The strain was identified as T. asperellum through using a combination of morphological characteristics and molecular data. T. asperellum was able to collapse the mycelium of the colonies of the pathogen through dual culture tests by breaking down the pathogenic hyphae into fragments. The scanning electron microscope showed that the hyphae of T. asperellum surrounded and penetrated the pathogens hyphae, resulting in hyphal collapse. The results show that seven days after inoculation, the hyphae of the pathogen were completely degraded in a dual culture. T. asperellum was also able to enter the P. capsici oospores through using oogonia and then developed hyphae and produced conidia, leading to the disintegration of the oogonia and oospores. Seven days after inoculation, an average 10.8% of the oospores were infected, but at this stage, the structures of oospores were still intact. Subsequently, the number of infected oospores increased and the oospores started to collapse. Forty-two days after inoculation, almost all the oospores were infected, with 9.3% of the structures of the oospores being intact and 90.7% of the oospores having collapsed.  相似文献   

Learning communities and fair trade in doctorates and development: report of a collaborative project     

Janinka Greenwood  Safayet Alam  Abu Nayeem Mohammad Salahuddin  Mollah Mohammed Haroon-Ar Rasheed 《Globalisation, Societies & Education》2016,14(1):49-67

This article reports the second stage of a study examining an academic partnership in which Bangladeshi doctoral students in a western university focus their research in the grounded context of Bangladesh and investigate the processes for change. After briefly outlining the previous published stage which examined the academic trade in higher education with developing countries, the article builds on the concept of fair academic trade to critically reflect on the development of a doctoral learning community, a publication project and three specific doctoral studies. The methodological approach is one of participatory action research, with focus on critical reflection on practice.  相似文献   

Learner satisfaction,engagement and performances in an online module: Implications for institutional e-learning policy     

Rajabalee  Yousra Banoor  Santally  Mohammad Issack 《Education and Information Technologies》2021,26(3):2623-2656

Education and Information Technologies - There has been debates related to online and blended learning from a perspective of learner experiences in terms of student satisfaction, engagement and...  相似文献   

Validity of acute phase proteins and trace metals as adjuncts in viral hepatitis A and B     

Saad A. Noeman  Kommajosyula Sharada  Hassan El-Bena  Samia EL Dardery  Khalid AL Sawaf 《Indian journal of clinical biochemistry : IJCB》1994,9(1):40-42

The level of seven acute phase proteins was estimated together with copper and zinc in two groups of 15 children each suffering from hepatitis A (HAV) or Hepatitis B (HBV). The mean concentration of α-1 antitrypsin, α-2 macroglobulin, ceruloplasmin, C3 and copper was increased uniformly in both the types of viral hepatitis. α-1 acid glycoprotein, haptoglobin and zinc levels were decreased in both the infections. A significant decrease in albumin level was observed in HAV while the decrease was not significant in HBV. All the paramenters were repeated after one month on the day of discharge following the disappearance of clinical signs and symptoms wherein the haptoglobin level returned to normal in HAV. No change was observed in the level of other acute phase proteins. The level of the trace elements returned to normal. The observed increase in the levels of acute phase proteins at the onset does not seem to have any significant relation to the subsequent course of the disease. However, the level of acute phase proteins and other biochemical parameters may indicate a delayed biochemical recovery phase in conjunction with the underlying hepatic pathological changes in the course of viral hepatitis in spite of clinical recovery.  相似文献