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Byung-Ju Jin Sailaja Battula Nick Zachos Olga Kovbasnjuk Jennifer Fawlke-Abel Julie In Mark Donowitz Alan S. Verkman 《Biomicrofluidics》2014,8(2)
Intestinal enteroids are ex vivo primary cultured single-layer epithelial cell spheroids of average diameter ∼150 μm with luminal surface facing inward. Measurement of enteroid swelling in response to secretagogues has been applied to genetic testing in cystic fibrosis and evaluation of drug candidates for cystic fibrosis and secretory diarrheas. The current measurement method involves manual addition of drugs and solutions to enteroids embedded in a Matrigel matrix and estimation of volume changes from confocal images of fluorescently stained enteroids. We developed a microfluidics platform for efficient trapping and immobilization of enteroids for quantitative measurement of volume changes. Multiple enteroids are trapped in a “pinball machine-like” array of polydimethylsiloxane posts for measurement of volume changes in unlabeled enteroids by imaging of an extracellular, high-molecular weight fluorescent dye. Measurement accuracy was validated using slowly expanding air bubbles. The method was applied to measure swelling of mouse jejunal enteroids in response to an osmotic challenge and cholera toxin-induced chloride secretion. The microfluidics platform allows for parallel measurement of volume changes on multiple enteroids during continuous superfusion, without an immobilizing matrix, and for quantitative volume determination without chemical labeling or assumptions about enteroid shape changes during swelling. 相似文献
453.
This article examines what has happened to training in public sector organisations in the UK in a period of austerity. It draws on individual-level data collected over the period 2000–2012 and establishment-level data collected from employer surveys carried out between 2005 and 2012. To understand these data further, 75 qualitative interviews with public sector employers were carried out between mid-2010 and early 2012. This article finds that while training incidence remained relatively high in the public sector, establishment-level control over planning and funding fell faster than in the private sector. Nevertheless, the public sector ethos of serving the community along with the tradition of the public sector as a ‘good employer’ meant that the training system within public sector organisations remained largely intact, even when the availability or frequency of some courses was reduced. The result was that limited training funds were made to go further by reducing the frequency of courses, prioritising courses immediately relevant to front-line services, tightening the application of eligibility criteria among potential trainees and economising on training delivery – summed up by one respondent as ‘training smarter’. 相似文献
454.
The football shirt is of iconic significance, defining a club's visual identity through its role as sporting uniform and fan identifier, providing a canvas for commercial interactions and increasingly acting as the focus of nostalgia and collector culture. In this article we focus on the football shirt's extension from sportswear to a replica product worn as cross-generational leisurewear. We first consider how a replica's authenticity, its principal attribute, exists in objective, constructive and existential contexts. We then demonstrate how the subsequent focus of kit manufacturers and clubs on satisfying these differing interpretations of authenticity has influenced football shirt design. For two decades, replica kits were marketed as sportswear to children, with attempts to enhance the football shirt's authenticity through distinctiveness and exclusivity leading to copyrighted designs, manufacturer's logos and increased patterning. However, as the replica football shirt became adult leisurewear, the changing customer base led to retrospective and recycled shirt designs proclaiming a club's distinct identity through its heritage, fulfilling fans' nostalgic interpretations of a club's authentic kit, and reflecting fans' use of replica shirts to display their authenticity as a genuine fan. These ideals have also inspired a parallel retro-replica industry and, we argue, caused stagnation in the development of the aesthetic elements of contemporary kit design, which had previously demonstrated innovation interspersed with periods of consolidation, but had taken few retrospective turns. Genuine innovation in football shirt design has increasingly become restricted to technological advances and marketing strategies. 相似文献
455.
Reed Nick Leiman Tania Palade Paula Martens Marieke Kester Leon 《Ethics and Information Technology》2021,23(4):777-789
Ethics and Information Technology - In this paper, we explore and describe what is needed to allow connected and automated vehicles (CAVs) to break traffic rules in order to minimise road safety... 相似文献
456.
This article reports the findings of a comparative study of teaching in Denmark and England. Its broader aim is to help develop an approach for comparing pedagogy. Lesson observations and interviews identified the range of goals towards which teachers in each country worked and the actions these prompted. These were clustered using the lens of Bernstein’s pedagogic discourse to construct teacher roles, which provided a view of pedagogy. Through this approach we have begun to identify variations in pedagogy across two countries. All teachers in this study adopted a variety of roles. Of significance was the ease with which competent English teachers moved between roles. The English teachers observed adopted roles consistent with a wider techno-rationalist discourse. There was a greater subject emphasis by Danish teachers, whose work was set predominantly within a democratic humanist discourse, whilst the English teachers placed a greater emphasis on applied skills. 相似文献
457.
Cyber crime poses an ever‐growing international threat to businesses. Organizations need to address this threat through a performance intervention by making managers aware of the reasons cyber crimes are committed so that they can detect and prevent them. This research details the primary types of cyber crime, describes and suggests managerial defenses to cyber crime, and reviews the importance of synchronized international legislation in the fight against cyber crime. 相似文献
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