What is the future of electrical impedance spectroscopy in flow cytometry?     

Furkan Gke  Paolo S. Ravaynia  Mario M. Modena  Andreas Hierlemann 《Biomicrofluidics》2021,15(6)

More than 20 years ago, electrical impedance spectroscopy (EIS) was proposed as a potential characterization method for flow cytometry. As the setup is comparably simple and the method is label-free, EIS has attracted considerable interest from the research community as a potential alternative to standard optical methods, such as fluorescence-activated cell sorting (FACS). However, until today, FACS remains by and large the laboratory standard with highly developed capabilities and broad use in research and clinical settings. Nevertheless, can EIS still provide a complement or alternative to FACS in specific applications? In this Perspective, we will give an overview of the current state of the art of EIS in terms of technologies and capabilities. We will then describe recent advances in EIS-based flow cytometry, compare the performance to that of FACS methods, and discuss potential prospects of EIS in flow cytometry.  相似文献   

Microliter-bioreactor array with buoyancy-driven stirring for human hematopoietic stem cell culture     

Camilla Luni  Hope C. Feldman  Michela Pozzobon  Paolo De Coppi  Carl D. Meinhart    Nicola Elvassore 《Biomicrofluidics》2010,4(3)

This work presents the development of an array of bioreactors where finely controlled stirring is provided at the microliter scale (100–300 μl). The microliter-bioreactor array is useful for performing protocol optimization in up to 96 parallel experiments of hematopoietic stem cell (HSC) cultures. Exploring a wide range of experimental conditions at the microliter scale minimizes cost and labor. Once the cell culture protocol is optimized, it can be applied to large-scale bioreactors for stem cell production at the clinical level. The controlled stirring inside the wells of a standard 96-well plate is provided by buoyancy-driven thermoconvection. The temperature and velocity fields within the culture volume are determined with numerical simulations. The numerical results are verified with experimental velocity measurements using microparticle image velocimetry (μPIV) and are used to define feasible experimental conditions for stem cell cultures. To test the bioreactor array’s functionality, human umbilical cord blood-derived CD34⁺ cells were cultured for 7 days at five different stirring conditions (0.24–0.58 μm∕s) in six repeated experiments. Cells were characterized in terms of proliferation, and flow cytometry measurements of viability and CD34 expression. The microliter-bioreactor array demonstrates its ability to support HSC cultures under stirred conditions without adversely affecting the cell behavior. Because of the highly controlled operative conditions, it can be used to explore culture conditions where the mass transport of endogenous and exogenous growth factors is selectively enhanced, and cell suspension provided. While the bioreactor array was developed for culturing HSCs, its application can be extended to other cell types.  相似文献   

A structural equation model predicting adults’ online learning self-efficacy     

Calaguas  Noriel P.  Consunji  Paolo Maria P. 《Education and Information Technologies》2022,27(5):6233-6249

Education and Information Technologies - We aimed to model the direct effects of the theorized relationships of academic self-efficacy, computer use self-efficacy, learning management system...  相似文献   

Development of a Three-Dimensional Phylogenetic Tree of the Plant Kingdom     

James E. Mickle  Paolo de Luca  Maria Rosaria Barone Lumaga  Manuela de Matteis Tortora 《Curator: The Museum Journal》1994,37(4):275-279

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