Evaluation of Imprecision,Bias and Total Error of Clinical Chemistry Analysers     

S. S. Biswas  M. Bindra  V. Jain  P. Gokhale 《Indian journal of clinical biochemistry : IJCB》2015,30(1):104-108

Context Two Biosystems analysers are used in our laboratory, a fully automated A25 and a semi-automated BTS-350. Internal quality control is done for both but external quality control only for A25. As BTS-350 is used for backup, it is important that the results of both analysers are not just comparable but also within predefined limits of systematic, random and total error (TE). Aim To evaluate the imprecision, bias and TE of the two Biosystem analysers. Materials and Methods Biosystems level-1 quality control sera lot number 70A was run in duplicate for 32 days on both the analysers. Between day imprecision (measured by the coefficient of variation), bias and TE were calculated for ten analytes and were checked to see whether they are within the acceptable minimum limits, desirable limits and optimum limits of allowable error based on specifications on Westgard’s website updated in 2014. Results On both the analysers, all the analytes except alkaline phosphatase were within the acceptable minimum limits of TE and most analytes were within the desirable limits of TE. Only TG on A25 was within the optimum limit of TE. Conclusion The two Biosystem analysers performed comparably with errors within acceptable limits for most analytes. BTS-350 was found to be a suitable and ready backup analyser for A25.  相似文献   

Effect of L-carnitine Supplementation on Nutritional Status and Physical Performance Under Calorie Restriction     

Swati Jain  Som Nath Singh 《Indian journal of clinical biochemistry : IJCB》2015,30(2):187-193

L-carnitine is popular as a potential ergogenic aid because of its role in the conversion of fat into energy. The present study was undertaken to investigate the effect of short term supplementation of L-carnitine on metabolic markers and physical efficiency tests under short term calorie restriction. Male albino rats were divided into four groups (n = 12 in each)—control, calorie restricted (CR for 5 days, 25 % of basal food intake), L-carnitine supplemented (CAR, given orally for 5 days at a dose of 100 mg/kg), CR with L-carnitine supplementation (CR + CAR). Food intake and body weight of the rats were measured along with biochemical variables like blood glucose, tissue glycogen, plasma and muscle protein and enzymatic activities of CPT-1 (carnitine palmitoyl transferase-1) and AMP kinase. Results demonstrated that L-carnitine caused marked increase in muscle glycogen, plasma protein, CPT-1 activity and swim time of rats (P < 0.05) on short term supplementation. In addition to the substantive effects caused by CR alone, L-carnitine under CR significantly affected muscle glycogen, plasma protein, CPT-1 activity and AMP kinase (P < 0.05). Short term CR along with L-carnitine also resulted in increased swim time of rats than control, CR and L-carnitine treated rats (P < 0.05). The present study was an attempt towards developing an approach for better adherence to dietary restriction regimen, with the use of L-carnitine.  相似文献   

The Role of 25-Hydroxy Vitamin D Deficiency in Iron Deficient Children of North India     

Shikha Sharma  Rahul Jain  Pradeep Kumar Dabla 《Indian journal of clinical biochemistry : IJCB》2015,30(3):313-317

Extensive data from animal and human studies indicate a role of vitamin D in erythropoiesis. Iron and vitamin D deficiencies are implicated with adverse health effects in children even if they are asymptomatic. The potential relationship between the two remains poorly understood. A cross-sectional study was performed in the period from 1st May 2012 through 30th April 2013 and subjects were classified into vitamin D deficiency (VDD), vitamin D insufficiency (VDI) and vitamin D sufficiency (VDS) groups according to their 25(OH) D levels. A total of 263 children were included in the analysis. Anaemia was present in 66 % of 25(OH) D deficient subjects compared with 35 % in vitamin D sufficient individuals (p < 0.0001). The association of breast feeding and development of VDD was also significant (p < 0.05). Serum levels of 25(OH) D were found lower in female sex and if the analysis was performed in the winter/spring season. Physicians should therefore assess vitamin D levels in all anaemic children and ensure adequate supplementation to prevent deficiencies.  相似文献   

Effect of ketoacids on H<Subscript>2</Subscript>O<Subscript>2</Subscript> induced cataract     

R. M. Jain  P. M. Bulakh 《Indian journal of clinical biochemistry : IJCB》2003,18(1):91-95

Cataract is one of the leading causes of visual disability often leading to blindness in the elderly population. One of the causes is oxidation of proteins present in lens, by hydrogen peroxide (H₂O₂). In the present study 100 goat lenses were analyzed to determine the protective efficacy of ketoacids, against the oxidative insult by H₂O₂. The ketoacids used were (pyruvate, alpha ketoglutarate and oxaloacetate), that are constantly produced endogenously. The lenses were incubated as control and experimental groups in TC-199 media for 72 hrs. H₂O₂ concentration of 10mM was used to induce cataract. The biochemical parameters measured were levels of malondialdehyde (MDA), a lipid peroxidation product and activity of glutathione peroxidase (G-Px), an enzymatic antioxidant. The results showed a significant increase in the levels of MDA and significant decrease in the activity of G-Px in the cataractous lenses as compared to control. After addition of ketoacids (pyruvate (10mM), alpha ketoglutarate (20mM) and oxaloacetate (20mM)) separately, the levels of MDA decreased significantly and the activity of G-Px increased significantly. The results suggest that the ketoacids can be very promising antioxidants for the treatment of cataract. They may also be useful in treating other disabilities related to acute and chronic oxidative stress.  相似文献   

#thisshowsucks! The Overpowering Influence of Negative Social Media Comments on Television Viewers     

T. Franklin Waddell  S. Shyam Sundar 《广播与电子媒介杂志》2017,61(2):393-409

Can the comments of a few viewers on social media affect viewers’ perceptions of audience sentiment or their own program enjoyment? If so, do the effects of comments vary based on their valence or placement during programming? We conducted a 2 (positive vs. negative tweets) x 2 (beginning vs. end of program) factorial experiment with an additional control condition (N = 196) to answer these questions. Negative comments undermined perceived bandwagon support for the program and reduced enjoyment, regardless of contextual or trait moderators. Findings suggest that the effects of social television may be attributable to systematic processing of bandwagon cues.  相似文献   

Using virtual laboratories in chemistry classrooms as interactive tools towards modifying alternate conceptions in molecular symmetry     

Krishnashree Achuthan  Vysakh Kani Kolil  Shyam Diwakar 《Education and Information Technologies》2018,23(6):2499-2515

Molecular symmetry plays a central role in chemistry education with regard to predicting chemical properties such as bonding and spectroscopic transitions. Better understanding of the symmetry of molecules requires high visual-spatial thinking ability. Conventional teaching methodologies, with limited teaching aides, fall short in providing a detailed understanding of scientific theories and related concepts. Incorrect understanding has been known to perpetrate concepts that are not consistent with the consensus of the research community or alternate conceptions. This work elaborates a methodology designed to discover the alternate conceptions stemming from teaching molecular symmetry in a typical classroom environment and the impact of the virtual laboratory (VL) environment in correcting these misconceptions. Three significant contributions presented in this paper include: (1) the development of a media and information-intense VL experiment platform designed to enhance understanding of symmetry elements and point groups of molecules with diverse structural geometries. (2) the development of an instrument, Molecular symmetry Alternate Conception Test (MACT), designed to capture and estimate the extent of alternate conceptions. (3) the successful identification of typical alternate conceptions amongst students in the context of molecular symmetry. In addition to perceived alternate concepts in symmetry education, the results indicate a significant statistical improvement of 156% in understanding of molecular symmetry concepts (p?<?0.05) after subjecting students to the interactive VL platform. This study also shows identifying bond angles and planarity as concepts crucial for students. It is also implicit that estimations of discrimination skills related to identifying concept-based learning may be relevant for perceiving alternate concepts among learners.  相似文献   

Three dimensional free convection couette flow with transpiration cooling     

JAIN N.C. GUPTA P. 《浙江大学学报(A卷英文版)》2006,7(3):340-346

INTRODUCTION In recent years, the subject of free convection flow has attracted the attention of a number of scholars because of its possible applications to several geophysical problems. In view of these applications, a series of investigations were made to study the flow past a vertical wall. Transpiration cooling can very effectively pro-tect certain structural elements in turbojet and rocket engines, like combustion chamber walls, exhaust nozzles or gas turbine blades from hot gases. …  相似文献   

Social and Technological Motivations for Online Photo Sharing     

Anne Oeldorf-Hirsch  S. Shyam Sundar 《广播与电子媒介杂志》2016,60(4):624-642

The popularity of social media sites for posting pictures has given rise to a new form of mediated communication: online photo sharing. This article explores motivations for sharing photos online, using the 2-step procedure common in Uses and Gratifications research. Focus groups revealed 42 motivations which were rated in importance by survey respondents (N = 460). Factor analysis revealed 4 classes of gratifications: seeking and showcasing experiences, technological affordances, social connection, and reaching out. These results suggest that photo sharing is driven by social needs and facilitated by interface features, with important implications for theories of technology and user psychology.  相似文献   

Rapid Detection of Mutation in RRDR of <Emphasis Type="Italic">rpo B</Emphasis> Gene for Rifampicin Resistance in MDR-Pulmonary Tuberculosis by DNA Sequencing     

Patra SK  Jain A  Sherwal BL  Khanna A 《Indian journal of clinical biochemistry : IJCB》2010,25(3):315-318

To detect the site of mutation in RRDR of rpo B gene for rifampicin resistance in MDR-TB by DNA sequencing. 50 MDR-TB patients were enrolled in our study after informed written consent. Mycobacterial DNA was extracted from sputum samples by Universal Sample Processing (USP) method and RRDR of rpo B gene was amplified by PCR using primers RP4T and RP8T and then sequenced by automated DNA sequencing. The nucleotide sequences of RRDR of rpo B gene were compared with the reference sequence. We observed three different types of mutation in the RRDR of rpo B gene. The frequency of mutation in codon 531 (TCG → TTG), 526 (CAC → TAC) and 516 (GAC → GTC) are 60, 26.6 and 6.6% respectively. Of the total cases studied, 6.6% cases, although resistant to rifampicin, did not show any mutation in the RRDR of rpo B gene. Codon 531 (TCG → TTG) is the most common site of mutation in RRDR of rpo B gene for rifampicin resistance in MDR-pulmonary tuberculosis followed by codon 526 (CAC → TAC) and codon 516 (GAC → GTC).  相似文献   

Induced charge electro-osmotic concentration gradient generator     

Mranal Jain  Anthony Yeung    K. Nandakumar 《Biomicrofluidics》2010,4(1)

Biomolecule gradients play an important role in the understanding of various biological processes. Typically, biological cells are exposed to linear and nonlinear concentration gradients and their response is studied for understanding cell growth, cell migration, and cell differentiation mechanisms. Recent studies have demonstrated the use of microfluidic devices for precise and stable concentration gradient generation. However, most of the reported devices are geometrically complex and lack dynamic controllability. In this work, a novel microfluidic gradient generator is presented which utilizes the induced charge electro-osmosis (ICEO) by introducing conducting obstacle in the microchannel. With the ICEO flow component, significant transverse convection can be generated within the microchannel, which can, in turn, be used to create nonlinear as well as asymmetric gradients. The characteristics of the developed concentration gradient are dependent on the interplay between fixed charge electro-osmotic and ICEO flows. It is shown that the proposed device can switch between linear and nonlinear gradients by just altering the applied electric field. Finally, the formation of user-defined concentration profiles (linear, convex, and concave) is demonstrated by varying the conducting obstacle size.  相似文献