Molecular mechanism of pathogenesis of dengue virus: Entry and fusion with target cell     

Seema  S. K. Jain 《Indian journal of clinical biochemistry : IJCB》2005,20(2):92-103

Dengue fever is one of the major health problems in India. Interaction with specific receptor(s) at the cell surface is one of the first events in the pathogenesis of Dengue virus. However, relatively little is known about these receptors. Cellular receptors in human monocytes and mouse neural cells are main target for the viral infection. The envelope protein of the virus (E-protein) plays important role in attachment of virus to target cells and their interaction with cellular receptors. The modulation of receptor gene(s) and/or protein(s) can be used as a method for interfering with virus entry and can thus become a new method for disease prevention. The receptors can be purified by affinity chromatography using E-protein as ligand. It has been reported that addition of highly sulfated heparan sulfate prevents E-protein binding to target cells suggesting that heparan sulfate is utilized by dengue envelope protein to bind to target cells.  相似文献   

Status of epidermal growth factor receptors family in hormone-dependent carcinomas of the breast and prostate with reference to serum lipids and lipoproteins     

D. Jain  A. Ray  A. K. Bahadur  K. U. Chaturvedi  R. Sood  S. Sharma  S. L. D. Naik  B. K. Sharma 《Indian journal of clinical biochemistry : IJCB》2001,16(1):42-51

There are numerous growing evidences of resemblance between carcinomas of the breast and prostate. A total of 45 cases of these two hormone-dependent cancers along with appropriate controls were subjected for status of epidermal growth factor receptors as well as serum lipid profile. Paraffin embedded tissue sections from aforesald tumours were analysed by immunohistochemical staining for epidermal growth factor receptor (EGF-R), c-erbB-2 oncoprotein, estrogen receptor (ER) and progesterone receptor (PgR). Sera from same individuals were studied for serum lipid profile analysis. The study revealed that immunoexpression of all receptor proteins (EGF-R). c-erbB-2 was significantly higher in breast carcinoma. In addition, mean levels of triglycerides, total cholesterol, LDL-cholesterol were found to be significantly elevated while the level of HDL-cholesterol was observed to be lower among patients with breast cancer as compared to matched controls. Further, ER-positive breast cancer cases have significantly higher mean level of HDL-cholesterol when compared with ER-negative breast cancer patients. Contrary to this, no alteration in different serum lipid fractions was noticed among the patients with prostate cancer. However, a positive relationship was noticed between immunoexpressions of EGF-R and c-erbB-2 in prostate cancer.  相似文献   

Optimization theory : by David L. Russell. 405 pages. New York,Benjamin, 1970. Price $7.95 (paper-back, $17.50 (hard cover) (approx. £3·15 and £6·32, respectively).     

Ramesh C. Jain 《Journal of The Franklin Institute》1973,296(3):222-223

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Correlation of Red Blood Cell Acetylcholinesterase Enzyme Activity with Various RBC Indices     

Shalvika Gupta  Vijetha Shenoy Belle  Ramya Kumbarakeri Rajashekhar  Sushma Jogi  RV Krishnananda Prabhu 《Indian journal of clinical biochemistry : IJCB》2018,33(4):445-449

Cholinesterases belongs to class hydrolases. There are two types acetylcholinesterase and butyryl cholinesterase. Acetylcholinesterase present in nerve endings and also in the RBC membrane. It helps to maintain the shape and size of RBCs. Any change in shape and size of RBCs may affect the activity of Acetylcholinesterase. Thus this study aimed to estimate RBCs Acetylcholinesterase enzyme activity in various types of anemias and correlate the RBCs Acetylcholinesterase enzyme activity with various hematological indices such as Erythrocyte Sedimentation Rate (ESR), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Red cell Distribution Width (RDW) etc. After obtaining ethical approval from Institutional ethics committee total of 100 samples were collected from Clinical Biochemistry laboratory, Kasturba Medical College, Manipal, Manipal University. 25 were having normal RBC indices, 12 with hemolytic anemia, 26 with microcytic anemia and 26 with macrocytic anemia based on peripheral smear report and RBC indices. Acetylcholinesterase were measured using Ellman’s method. RBC acetylcholinesterase activity was significantly increased in microcytic anemia (58.13 ± 5.4) and macrocytic anemia (76.87 ± 6.7) than normal group (37.62 ± 2.71). Also increased RBC acetylcholinesterase was seen in hemolytic anemia (48.11 ± 5.18) but the increase is not statistically significant. RBC acetylcholinesterase correlated negatively with hemoglobin (r = ?0.356, p = 0.001) and positively with RDW (r = 0.31, p = 0.003). To conclude RBC acetylcholinesterase activity can be used as one of the potential marker for various types of anemia.  相似文献   

Hypoglycemic, lipid lowering and antioxidant activities in root extract of Anthocephalus indicus in alloxan induced diabetic rats     

Vishnu Kumar  A. K. Khanna  M. M. Khan  Ranjana Singh  Sushma Singh  Ramesh Chander  Farzana Mahdi  J. K. Saxena  Shweta Saxena  V. K. Singh  R. K. Singh 《Indian journal of clinical biochemistry : IJCB》2009,24(1):65-69

The present study was carried out to evaluate the hypoglycemic, lipid lowering and antioxidant activities in root extract of Anthocephalus indicus (A indicus) in alloxan inducd diabetic rats. Oral administration of ethanol extract of root (500mg/ kg body weight) for 21 days resulted in significant decrease in the levels of blood glucose, triglycerides, total cholesterol, phospholipid and free fatty acids. Furthermore, the root extract (100–400μg) inhibited the generation of superoxide anions and hydroxyl radicals, in both enzymic and non-enzymic systems, in vitro. The result of the present study demonstrated hypoglycemic, lipid lowering and antioxidant activities in root extract of A indicus, which could help in prevention of diabetic dyslipidemia and related diseases.  相似文献   

Induced charge electro osmotic mixer: Obstacle shape optimization     

Mranal Jain  Anthony Yeung    K. Nandakumar 《Biomicrofluidics》2009,3(2)

Efficient mixing is difficult to achieve in miniaturized devices due to the nature of low Reynolds number flow. Mixing can be intentionally induced, however, if conducting or nonconducting obstacles are embedded within the microchannel. In the case of conducting obstacles, vortices can be generated in the vicinity of the obstacle due to induced charge electro-osmosis (ICEO) which enhances mixing of different streams: the obstacle shape affects the induced zeta potential on the conducting surface, which in turn influences the flow profile near the obstacle. This study deals with optimization of the geometric shape of a conducting obstacle for the purpose of micromixing. The obstacle boundary is parametrically represented by nonuniform rational B-spline curves. The optimal obstacle shape, which maximizes the mixing for given operating conditions, is found using genetic algorithms. Various case studies at different operating conditions demonstrated that the near right triangle shape provides optimal mixing in the ICEO flow dominant regime, whereas rectangular shape is the optimal shape in diffusion dominant regime. The tradeoff between mixing and transport is examined for symmetric and nonsymmetric obstacle shapes.  相似文献   

Serum ionised calcium in birth asphyxia     

B. K. Jain  Daljit Singh  Harmesh Singh  A. P. S. Narang  Praveen C. Sobti  Ajay Gupta 《Indian journal of clinical biochemistry : IJCB》2000,15(1):36-39

Serum total and ionised calcium levels were measured at birth and at 48 hours in 25 term neonates with birth asphyxia (one minute APGAR score of 6 or less) and in 25 normal term neonates (one minute APGAR score of 7 or more). Infants were categorised into two groups TAGA (term appropriate for gestational age) and TSGA (term small for gestational age). Asphyxiated infants had significantly lower serum total and ionised calcium values at birth as well as at 48 hours. Abnormal clinical features were observed in 48% of asphyxiated infants. Low ionised calcium was detected in symptomatic babies, who had otherwise normal total calcium values. Due to hyocalcemia especially ionised calcium in asphyxiated infants and high frequency of functional derangement associated with this hypocalcemioa, serial monitoring of serum isonised calcium levels is necessary.  相似文献   

Studies on a new antitubercular drug, trifluoperazine: Its effect in combination with conventional antitubercular drugs in tubercular lymphadenitis   总被引：1，自引：1，他引：0  

R. L. Gupta  Sundeep Jain  V. Talwar  H. C. Gupta  P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》1998,13(2):92-97

After demonstrating that trifluoperazine (TFP) possesses invitro antitubercular activity against drug (single and multidrug) resistantMycobacterium tuberculosis, we initiated preliminary clinical studies in a few patients of tubercular lymphadenitis. Effect of TFP was assessed by testing the antitubercular activity of the serum of patients receiving TFP in addition to regular therapy. Patients were divided into two groups of 30 each. For ethical considerations, patients of both groups were treated initially for one month with antitubercular therapy (ATT) consisting of isoniazid, rifampicin, ethambutol and pyrazinamide and TFP was tried for 15 days only. Patients of group1 were given a single dose of TFP (5mg/day) daily from days 31 to 45 in addition to ATT, while those in group 2 received ATT only. Assessment of the antitubercular activity of the serum (testedin vitro in Youmans and Karlson’s liquid medium) revealed that the serum of patients (collected on 45th day) of group1 (ATT+TFP treated) possessed much higher antitubercular activity than that of group 2 (ATT only treated) patients. Clinical examination indicated that overall improvement was seen much earlier in group1 (ATT+TFP) patients than in group 2 (ATT alone) patients. At the end of the follow up period of 6 months with ATT from 46th day onwards to both groups, there were no side effects due to TFP. Hematology and liver function tests were normal in both the groups. We suggest that TFP has good potential and therefore deserves further studies either in combination with other drugs of ATT or as one of the drugs of ATT, for the treatment of tuberculosis due to MDR strains to find a suitable effective dose without side effects.  相似文献   

Critical Race Theory and the Transfer Function: Introducing a Transfer Receptive Culture     

Dimpal Jain  Alfred Herrera  Santiago Bernal  Daniel Solorzano 《Community College Journal of Research & Practice》2013,37(3):252-266

In California, the majority of students of color who enter postsecondary education do so in the community colleges. However large numbers of them leave and do not transfer to four-year institutions; in particular to highly selective public four-year colleges and universities. By using the theoretical perspective of critical race theory, transfer can be seen as a dual commitment between both the sending and receiving institution as we put forth the conceptual framework of a “transfer receptive culture.” We define a transfer receptive culture as an institutional commitment by a four-year college or university to provide the support needed for students to transfer successfully—that is, to navigate the community college, take the appropriate coursework, apply, enroll, and successfully earn a baccalaureate degree in a timely manner. We outline five elements, informed by critical race theory, that are necessary to establish a transfer receptive culture and outline specific strategies within each element that practioners can employ on their home campuses.  相似文献   

Measurement of Attitudes of Rural Women Towards Self-Help Groups     

M.S. Meena  Dilip Jain  H.R. Meena 《The Journal of Agricultural Education and Extension》2013,19(3):217-229

Abstract

Self-help groups (SHGs) have emerged as an effective mechanism of empowerment and development of women as well as being on efficient mode of promoting group action and technology dissemination. Initiatives were undertaken at the Central Institute of Post-Harvest Engineering and Technology (CIPHET), Ludhiana to facilitate the formation of women's SHGs and to develop their capabilities through training programmes for harnessing the benefits of value addition and processing technologies for employment and income generation. The study attempts to assess the attitude changes in women about SHGs as a result of training. The evaluation of SHGs was conducted at attitude construct. A Likert Likert, R. 1932. A Technique for the Measurement of Attitudes. Archives of Psychology, 140: 44–53.  [Google Scholar]-type scale consisting of 26 items was developed, for which Cronbach's alpha coefficient of reliability was observed as 0.85. The survey instrument contained five sections; namely, socio-economic upliftment; education and training; marketing and entrepreneurship qualities; technology adoption and participatory research; and banking/credit aspects. The training group consisting of 30 participants of SHGs responded to five-section survey instrument. Significant t-test results for mean values of attitude of women before and after the training showed a significant change in attitude of women in all the five areas as a result of training. Positive orientation towards a task paves the way for success. The study exemplifies the impact of training in instilling positive orientation. For addressing the issues of rural poverty, enrichment of the system with social capital through empowerment and formation of women SHGs; provision of financial and credit support; creation of market-driven and decentralized extension system; use of media-mix for technology transfer and informal education at rural level; conduction of need-based training and strong political will need to be emphasized.  相似文献