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Zhichang Qiu Long Tu Liang Huang Taoyuanmin Zhu Volker Nock Enchao Yu Xiao Liu Wenhui Wang 《Biomicrofluidics》2015,9(1)
Optogenetics has been recently applied to manipulate the neural circuits of Caenorhabditis elegans (C. elegans) to investigate its mechanosensation and locomotive behavior, which is a fundamental topic in model biology. In most neuron-related research, free C. elegans moves on an open area such as agar surface. However, this simple environment is different from the soil, in which C. elegans naturally dwells. To bridge up the gap, this paper presents integration of optogenetic illumination of C. elegans neural circuits and muscular force measurement in a structured microfluidic chip mimicking the C. elegans soil habitat. The microfluidic chip is essentially a ∼1 × 1 cm2 elastomeric polydimethylsiloxane micro-pillar array, configured in either form of lattice (LC) or honeycomb (HC) to mimic the environment in which the worm dwells. The integrated system has four key modules for illumination pattern generation, pattern projection, automatic tracking of the worm, and force measurement. Specifically, two optical pathways co-exist in an inverted microscope, including built-in bright-field illumination for worm tracking and pattern generation, and added-in optogenetic illumination for pattern projection onto the worm body segment. The behavior of a freely moving worm in the chip under optogenetic manipulation can be recorded for off-line force measurements. Using wild-type N2 C. elegans, we demonstrated optical illumination of C. elegans neurons by projecting light onto its head/tail segment at 14 Hz refresh frequency. We also measured the force and observed three representative locomotion patterns of forward movement, reversal, and omega turn for LC and HC configurations. Being capable of stimulating or inhibiting worm neurons and simultaneously measuring the thrust force, this enabling platform would offer new insights into the correlation between neurons and locomotive behaviors of the nematode under a complex environment. 相似文献
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How do visitors to fine art museums experience exhibitions? Can we classify their experiences? What are the factors that drive different types of visitor experience? We set out to answer these questions by analyzing from sociological, psychological, physiological, and behavioral perspectives the responses of 576 visitors to a special exhibition 11: 1 (+ 3) = Eleven Collections for One Museum mounted at the Kunstmuseum St. Gallen, Switzerland, from June to August 2009. Our five‐year research project, eMotion: Mapping the Museum Experience, interpreted computer‐modeled movement‐tracking and physiological maps of the visitors in complement with entrance and exit surveys. We tested individual aspects of the visitor, such as her or his expectations of the exhibition prior to seeing it; his or her socio‐demographic characteristics; her or his affinity for art, mood just before and receptivity just after the visit; and spatial, individual, and group‐related behavior patterns. Our study breaks down three types of exhibition experience that we call “the contemplative,” “the enthusing,” and “the social experience.” The results yield new information about aesthetic arousal, cognitive reaction, patterns of social behavior, and the diverse elements of the exhibition experience. 相似文献
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AbstractMathematics for liberal arts courses are a staple of most mathematics departments and are often populated with students who have disengaged from mathematics. Inquiry-based learning is a student-centered pedagogical tool for re-engaging students in mathematics. In this paper, we introduce a Special Issue of PRIMUS on “Using Inquiry-Based Learning in Mathematics for Liberal Arts Courses.” 相似文献
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Volker Gruhn 《Informatik - Forschung und Entwicklung》1996,11(2):94-101
Zusammenfassung. In diesem Artikel wird ein Ansatz zur Entwicklung von Software, die auf die Unterstützung interpersoneller Gesch?ftsprozesse
abzielt, vorgestellt. Dieser Ansatz basiert auf der Modellierung der zu unterstützenden Gesch?ftsprozesse, auf der Analyse
von Gesch?ftsproze?modellen und auf der Durchführung realer Gesch?ftsprozesse auf Basis dieser Modelle. Der FUNSOFT-Netz-Ansatz
wird als Realisierung des genannten Vorgehens vorgestellt. Seine Anwendung wird mit Hilfe eines Beispiels aus dem Bereich
der Bau- und Wohnungswirtschaft er?rtert.
Eingegangen am 15. April 1995 / Angenommen am 11. Dezember 1995 相似文献
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Dynamic balance performance is neither the objective of conscious actions nor the result of unwitting behavior (cf. Groeben & Scheele, 1993). It is more appropriately conceptualized as an affordance extraction and in each mode of coordination is subject to the specific demands of personal, task-related, and environmental conditions (Newell, 1984, 1996; Nitsch, 1995). Skilled balance performance is thus never achieved for its own sake but is rather integrated in the functional interrelationship of activity goals. Unlike the concept of resources, additional tasks as viewed from this perspective do not necessarily decrease balance performance, but can even serve as positive motivation. Therefore, it is justifiable to raise doubts about a paradigm where the test subjects are required to sway as little as possible during measurement. We assume that postural control is not autonomous, but rather functional as an integrated part of a specific system of motion information (Bootsma, 1998). With these theoretical assumptions in mind, some of the research approaches that abound in the literature are presented and our own research strategy that is intended to be more ecological is outlined. 相似文献