Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein     

罗本燕  徐增斌  陈智  陈峰  唐敏 《浙江大学学报(A卷英文版)》2004,5(8):989-994

Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution.  相似文献   

仙道小说中服食松柏成仙情节的现实背景     

唐娜 《南京师范大学文学院学报》2007,(1):24-27

现实生活中,在灾荒之年,人们往往会以松柏为食充饥;而在传统医学文献中,松柏还有治病的功效。魏晋之际,道教迅速发展,贵族与平民分别在自己生活的基础上创造了一些食松柏而成仙的故事,食用松柏成为道教修道成仙的一种方法。  相似文献   

Relationship between the size of pontocerebellar angle tumor and audiology     

Tang Jian-guo  Cai Yi 《浙江大学学报(A卷英文版)》2001,2(1):111-113

Objective: To study the relationship between the size of pontocerebellar angle tumor and audiology. Methods: Retrospective analysis of accoustically evoked brainstem response (ABR) waveforms and pure tone threshold in 27 subjects with tumor of pontocerebellar angle. Results: ABR wave forms and pure tone threshold were significantly affected statistically by the size of tumors, especially those tumor larger than 3 cm in diameter. Conclusion: The primary symptom of the patient was unilateral hearing loss. Early discovery of the lesion is important and ABR is a sensitive tool for early diagnose of the tumor.  相似文献   

加入WTO后政府与民办高校的关系定位     

朱君义  唐毅  余芳 《教育导刊》2002,(8):70-72

中国加入WTO后,中国教育势必融入世界教育市场.这一形势对规范我国政府和民办高校的关系提出了严峻的挑战.本文针对目前我国民办高校面临的形势、我国政府和民办高校关系存在的问题,就政府与民办高校之间的关系定位进行探讨.  相似文献   

Grant‐maintained Heads’ Reflections on Opting Out     

Jonathan Cauldwell  Ivan Reid 《Educational studies》1996,22(2):245-260

This article summarises the views of 222 grant‐maintained (GM) school's head teachers’ experience of, and reasons for, their schools opting out of local authority control. The survey was conducted on the first 499 secondary schools to go GM. The article investigates issues such as admissions, finances and also the effects of GM status (GMS) on the relationships with, and roles of, local education authorities, the Department for Education, parents and governors. The article provides an overview of moves to GMS and highlights prior aims and reflections.  相似文献   

Web & wild     

Kuo Zhang  Hui Xu  Jie Tang  Juanzi Li 《TechTrends》2000,44(6):48-48

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Impact of Anatomy Boot Camp on Students in a Medical Gross Anatomy Course          下载免费PDF全文

Patrick J. Herling  B. Tanya Mohseni  Derek C. Hill  Stacy Chelf  Jeffrey A. Rickert  Jonathan T. Leo  Natalie R. Langley 《Anatomical sciences education》2017,10(3):215-223

Lincoln Memorial University‐DeBusk College of Osteopathic Medicine (LMU‐DCOM) offers an optional three‐week summer Anatomy Boot Camp course (ABC) to facilitate students' transition into medical school and promote retention of anatomy subject matter. The pre‐matriculation program is a supplemental instruction course that utilizes a small group learning format. Boot camp instruction is led by teaching assistants and two anatomy professors. Enrollees gain early exposure to Medical Gross Anatomy (MGA) course subject matter, which is taught in the fall semester, and learn study skills necessary to excel in medical school. No grade is assigned for the course, therefore participants can study without the fear of potentially affecting grades. This study evaluates the effectiveness of the LMU‐DCOM ABC course using data from four consecutive summers. Independent two‐sample t‐tests were used to compare ABC to non‐ABC students for the following variables: incoming grade point average (GPA) and Medical College Admission Test® (MCAT®) scores, MGA written and laboratory practical examination grades, and final MGA course grade. Additionally, a 26‐question survey was administered to 2012–2014 boot camp participants. There were no significant differences in incoming GPA and MCAT scores. However, boot campers scored significantly higher on the first two lecture and laboratory examinations (P < 0.05) for each year of the study. Thereafter scores varied less, suggesting a faster head start for boot camp participants. Mean MGA final grade was on average 3% higher for the boot camp cohort. The survey feedback supports that the ABC course assists with the academic and social transition into medical school. Anat Sci Educ 10: 215–223. © 2016 American Association of Anatomists.  相似文献   

创建学习型学校的理性思考     

张扬群  汤光亮 《成都教育学院学报》2006,20(3):22-24,78

学习型学校是指通过培养教师的自主学习和团队学习,形成学校浓厚的学习气氛,进而充分发挥教师的创造性思维能力,为实现学校的共同愿景而创造性工作的学校。马克思主义哲学是创建学习型学校的哲学依据,彼得·圣吉的学习型组织理论是创建学习型学校的理论依据。新时代对教育“挑战”使我们认识到:创建学习型学校是建设学习化社会的需要,是提高办学水平的需要,是提高教师素质的需要。创建学习型学校应树立现代化的学习理念,营造学习型校园文化,建构灵活高效的学习机制,确立学校共同发展的愿景,建立自主管理机制,构筑学习评价体系。  相似文献   

Color control of the multi-color printing device   总被引：4，自引：0，他引：4  

WANG Xiao-hua XIU Xiao-jie ZHU Wen-hua TANG Hong-jun 《浙江大学学报(A卷英文版)》2006,7(7):1187-1192

INTRODUCTION During the development of printing technology, digital printing has played an important role. Now various kinds of multi-color digital inkjet printers are available. Compared with the conventional digital inkjet printer, the multi-color digital inkjet printer has larger color gamut and can faithfully produce more colors. The conventional color printing devices nor- mally use three or four inks, e.g., CMY, CMYK. Although some adopt more than four inks, e.g., CMYKLcLm, the…  相似文献   

生物学教学中合作学习策略初探     

吴文荣  翁望江  唐燕 《中学生物学》2007,23(3):62-64

合作学习是新课程理念的新颖产物，合作学习作为一种新理念正在广泛地应用于课堂教学中。就合作学习在生物学教学中如何设计教学的整体目标、如何设计教学课型进行了初步的探索，试图找到一条即符合客观规律，又适合自己和本校学生特点的方法，使每个学生在参与的过程中体验学习的快乐。促进学生形成良好的非认知品质。  相似文献