刺五加多糖对人白血病K562细胞作用的研究     

罗强  孙黎  刘芳  薄爱华 《河北北方学院学报(医学版)》2008,25(5)

目的:研究刺五加多糖对体外培养人白血病K562细胞有无增殖抑制和凋亡诱导作用。方法:取培养至对数生长期的K562细胞(密度为5×104/mL和1×106/mL)分别接种于96孔培养板(100μL/孔)及50mL培养瓶(1.5mL/瓶)中,加入不同浓度的刺五加多糖作用24h后,用CCK-8法检测刺五加多糖对K562细胞增殖抑制作用;荧光显微镜检测细胞凋亡。结果:不同浓度刺五加多糖(0.405、0.810、1.620、2.430、3.240mg/mL)作用K562细胞24 h,抑制率分别为16%、27%、48%、50%、55%;荧光显微镜下观察发现培养K562细胞中出现核固缩、凋亡小体。结论:刺五加多糖对体外培养K562细胞生长有明显的抑制作用,可诱导K562细胞凋亡。  相似文献   

研究生教育质量的指数测度方法——对“985工程”一期教育部直属高校的实证分析     

翟亚军  王战军  彭方雁 《教育研究》2012,(2):79-83

研究生教育质量指数既是一个概念,也是一种方法。研究生教育质量指数是指反映不同时间或空间条件下研究生教育质量变动方向和变动程度的相对数。研究生教育质量指数可以分为个体指数、组指数和总指数指标,其选取原则为:泛学科色彩、全面与重点相统一以及突出监测性和预警性指标。选取生师比、生均科研经费、资产总额三项指标,编制了"985工程"一期29所教育部直属高校的研究生教育质量总指数,并利用3σ方法进行警情分析。这项工作对于保障我国研究生教育的稳步发展,提高研究生教育质量具有重要意义。  相似文献   

臀肌挛缩发病聚集区人群干预效果的前瞻性观察   总被引：1，自引：0，他引：1  

王剑  孔繁增  苏峰  申永清  金春亭 《河北北方学院学报(医学版)》2007,24(2):5-8

目的：利用控制可疑致病因素，进行人群干预试验并观察效果，探讨臀肌挛缩症的病因及控制方法。方法：在控制苯甲醇青霉素肌肉注射这一因素后，从2001-2005年连续五年对病区进行观察，通过计算发病率和患病率来探讨该干预措施的效果。结果：苯甲醇青霉素肌肉注射是一种危险的致病因素，但不是唯一的、必须的致病因素。结论：臀肌挛缩症是一种社会性疾病，要杜绝此病的群体发生，除了药物因素，社会因素更重要。  相似文献   

Visual and auditory coding confusability in students with and without learning disabilities     

B W Hardy  C W McIntyre  A S Brown  A J North 《Journal of learning disabilities》1989,22(10):646-651

The nature of visual and auditory coding processes in students with learning disabilities (SLDs) and student controls (SCs) was examined with a letter-matching task on four types of successively presented letter pairs: identical (A,A), visually confusable (P,R), auditorily confusable (F,S), and neither visually nor auditorily confusable (N,T). Two delay intervals (0 and 2 seconds) were used between the presentation of the first and second letters. Analysis of decision latencies on the nonidentical letter pair trials revealed that with initial exposure to the task, the SLDs responded more slowly than SCs, but their general confusability patterns (visual and auditory) were similar. With additional practice, overall decision latencies were comparable for the two groups, while confusability differences emerged: SCs showed maximal visual confusability at a 0-second delay and maximal auditory confusability at a 2-second delay, while SLDs did not. Evidently, SLDs make less extensive use of visual and auditory coding processes compared to SCs.  相似文献   

大鼠脑挫伤后"即早基因"-c-jun和c-fos基因表达的特点     

薄爱华  董建峰  张辉  邢立强  米坤龙 《河北北方学院学报(医学版)》2004,21(5):12-14

目的:探讨脑挫裂伤后c-jun和c-fos基因表达的特点.方法:利用自由落体法复制大鼠脑挫裂伤动物模型.伤后30′、1h、2h、4h、8h、24h和72h杀死大鼠取脑组织,石蜡切片,用PV6000免疫组织化学染色.结果:正常对照组假手术组c-fos无阳性表达,而c-jun呈弱阳性.模型组c-fos阳性神经元分布在伤灶周围区皮质1-2mm范围和部分海马;c-jun阳性神经元在伤侧皮质和海马广泛分布.结论:c-jun基因表达较c-fos更强烈、更广泛和表达时间更长.  相似文献   

DNA拓扑异构酶Ⅱ在膀胱癌中表达的临床意义   总被引：2，自引：0，他引：2  

张江兰  陈秀玲  薄爱华  张晓丽  邢立强 《河北北方学院学报(医学版)》2005,22(3):14-15

目的:研究TopoⅡ在膀胱癌中表达的意义.方法:应用免疫组化SP法,检测72例膀胱癌组织中TopoⅡ的表达.结果:TopoⅡ在膀胱癌中的阳性表达率为45.83%(33/72).TopoⅡ的表达与膀胱癌的分级呈正相关(P＜0.05),与膀胱癌的分期无显著性差异(P＞0.05),但随分期的递增呈升高趋势.结论:TopoⅡ的表达与癌组织的分级和分期有关.检测TopoⅡ表达情况可为抗癌药物的筛选提供依据.  相似文献   

Music Selection Behaviors in Everyday Listening     

Amanda Krause  Adrian North  Lauren Hewitt 《广播与电子媒介杂志》2013,57(2):306-323

Data on everyday music listening obtained via the Experience Sampling Method indicated that selection method was related to liking for and emotional response to the music, attention paid to the music, and perceived consequences of hearing the music. Individual listener's characteristics (e.g., age and level of engagement with music) were associated with selection behaviors. Negative effects resulted when individuals perceived they did not have control over music selection. In contrast, possessing control led to positive consequences such as enjoyment and motivation. These results indicate that control is an important aspect of one's everyday music experiences.  相似文献   

Differences in step characteristics and linear kinematics between rugby players and sprinters during initial sprint acceleration     

James J. Wild  Ian N. Bezodis  Jamie S. North  Neil E. Bezodis 《European Journal of Sport Science》2018,18(10):1327-1337

The initial steps of a sprint are important in team sports, such as rugby, where there is an inherent requirement to maximally accelerate over short distances. Current understanding of sprint acceleration technique is primarily based on data from track and field sprinters, although whether this information is transferable to athletes such as rugby players is unclear, due to differing ecological constraints. Sagittal plane video data were collected (240?Hz) and manually digitised to calculate the kinematics of professional rugby forwards (n?=?15) and backs (n?=?15), and sprinters (n?=?18; 100?m personal best range?=?9.96–11.33?s) during the first three steps of three maximal sprint accelerations. Using a between-group research design, differences between groups were determined using magnitude-based inferences, and within-group relationships between technique variables and initial sprint acceleration performance were established using correlation. Substantial between-group differences were observed in multiple variables. Only one variable, toe-off distance, differed between groups (d?=??0.42 to ?2.62) and also demonstrated meaningful relationships with sprint performance within all three groups (r?=??0.44 to ?0.58), whereby a stance foot position more posterior relative to the centre of mass at toe-off was associated with better sprint performance. While toe-off distance appears to be an important technical feature for sprint acceleration performance in both sprinters and rugby players, caution should be applied to the direct transfer of other kinematic information from sprinters to inform the technical development of acceleration in team sports athletes.  相似文献   

宫颈鳞癌中耐药标志物HSP27和MT的表达及意义   总被引：1，自引：0，他引：1  

姬宏宇  张国徽  薄爱华  李建平  成日青  张晓莉  左东升  李海峰 《河北北方学院学报(医学版)》2004,21(5):58-62

目的:探讨子宫颈癌变过程中,耐药标志物热休克蛋白27(heat shock protein 27,HSP27)和金属硫蛋白(metallothionein MT)的表达特点,为指导子宫颈癌患者化疗方案制定和提示预后积累资料.方法:集子宫颈切除及活检标本118例,其中有正常宫颈组织13例;子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)25例;子宫颈鳞状细胞癌80例.在鳞癌组中高分化鳞癌13例、中分化鳞癌53例、低分化鳞癌14例.以S-P免疫组织化学方法,显示HSP27和MT的表达水平.结果:染色显示细胞核或细胞浆被染呈大小不一,深浅不等的棕黄色颗粒.HSP27在正常宫颈上皮、CIN和宫颈鳞癌中阳性率分别为7.69%、24.00%和46.25%,三组间差异有统计学意义(P＜0.01).在正常宫颈中MT阳性率为7.69%(1/13),CIN中阳性率为28.00%(7/25),在宫颈鳞癌中阳性率为56.25%(45/80),三者间有显著性差异(P＜0.01).HSP27和MT的阳性表达率在不同分化程度的宫颈鳞癌间无显著差异.结论:HSP27和MT在正常宫颈、宫颈上皮内瘤变、宫颈鳞癌的阳性表达率逐渐升高(P＜0.01).HSP27和MT的过度表达可分别或联合作为临床上早期诊断宫颈癌或判断CIN预后的参考指标.  相似文献   

概念图的理论及其在教学中的应用   总被引：14，自引：3，他引：14  

王大平  李新国 《现代教育技术》2004,14(6):45-48

概念图已经开始为越来越多的人所关注。现代认知主义学习理论和建构主义学习理论都非常好的支持概念图的教学意义。通过概念图进行学习,能提高学生的理解能力和记忆能力,有助于培养学生的逻辑思维和创造性思维。本文主要探讨概念图的理论基础、概念图在教学中的作用和意义、并具体介绍了概念图在实际教学中的应用情况。  相似文献