An Integrated Approach of the Potential Underlying Molecular Mechanistic Paradigms of SARS-CoV-2-Mediated Coagulopathy     

Anmol Goyal  Rajendra Prasad  Parul Goel  Amit Pal  Suvarna Prasad  Isha Rani 《Indian journal of clinical biochemistry : IJCB》2021,36(4):387

Coronavirus disease 2019 (Covid-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a pandemic disease which has affected more than 6.2 million people globally, with numbers mounting considerably daily. However, till date, no specific treatment modalities are available for Covid-19 and also not much information is known about this disease. Recent studies have revealed that SARS-CoV-2 infection is associated with the generation of thrombosis and coagulopathy. Fundamentally, it has been believed that a diverse array of signalling pathways might be responsible for the activation of coagulation cascade during SARS-CoV-2 infection. Henceforth, a detailed understanding of these probable underlying molecular mechanistic pathways causing thrombosis in Covid-19 disease deserves an urgent exploration. Therefore, in this review, the hypothetical crosstalk between distinct signalling pathways including apoptosis, inflammation, hypoxia and angiogenesis attributable for the commencement of thrombotic events during SARS-CoV-2 infection has been addressed which might further unravel promising therapeutic targets in Covid-19 disease.  相似文献   

Rapid and cost-effective fabrication of selectively permeable calcium-alginate microfluidic device using "modified" embedded template method     

Asthana A  Ho Lee K  Kim KO  Kim DM  Kim DP 《Biomicrofluidics》2012,6(1):12821-128219

In this paper, we have presented a non-lithographic embedded template method for rapid and cost-effective fabrication of a selectively permeable calcium-alginate (Ca-alginate) based microfluidic device with long serpentine delay channel. To demonstrate the versatility of the presented method, we have demonstrated two different strategies to fabricate serpentine long delay channels without using any sophisticated microfabrication techniques, in formal lab atmosphere. The procedure presented here, also, enables the preparation of a multilayered microfluidic device with channels of varying dimensions, in a single device without using any sophisticated micromachining instrumentation. In addition, we have also qualitatively studied the diffusion of small and large molecules from a Ca-alginate based microfluidic device and proposed a method to effectively control the out-flow of macro biomolecules from the crosslinked Ca-alginate matrix to create a selectively permeable matrix required for various biological and biomimetic applications, as mentioned in the Introduction section of this work.  相似文献   

Co-inheritance of HbD Iran/Beta Thalassemia IVS1–5 (G?>?C) Trait in a Punjabi Lady with Diabetes     

Vijay S. Bhat  Amit Kumar Mandal  Bobby Mathew 《Indian journal of clinical biochemistry : IJCB》2012,27(2):202-206

The present report describes the molecular study of HbD ^Iran (beta) 22 Glu → Gln associated with β-Thalassemia IVS1–5 (G > C) found in India, and the first case in which mutation has been identified using mass spectrometry. Given the apparent ethnic origin and the mobility of the variant hemoglobin at alkaline pH, hemoglobin D-Punjab would be suspected, but HPLC excluded this possibility. Further characterization of hemoglobinopathy was made by using nondenaturing gel electrophoresis and matrix assisted laser desorption ionization mass spectrometry and IVS1–5 being validated by reverse dot blot hybridization followed by sequencing of the β-globin gene.  相似文献   

Maturity models in LIS study and practice     

Amit Tiwari  Devika P. Madalli 《Library & information science research》2021,43(1):101069

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Discovery of parity violation     

Amit Roy 《Resonance》2005,10(12):164-175

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Characterization of a Double Heterozygote HbE/β+ Thalassemia IVS 1-1 [G>T] in a Juvenile Diabetic     

Vijay S. Bhat  S. Lokeshwarappa  Amit Kumar Mandal 《Indian journal of clinical biochemistry : IJCB》2015,30(2):238-242

The present case report describes the molecular and proteomic based study of Hb variant HbE associated with β⁺ thalassemia IVS 1-1 G>T, in a juvenile diabetic patient. Given the ethnic origin and mobility of the variant hemoglobin at alkaline pH, HbE would be suspected. But hematologically and clinically abnormality being detected, HPLC and Electrophoresis not being able to characterize due to retention time and band being in region of HbA2, respectively, further characterization of hemoglobinopathy was made using MALDI and IVS 1-1 G>T being validated by reverse dot blot hybridization. Capillary electrophoresis was also employed in order to separate HbE and HbA2 bands. This case report being first of its kind, wherein a HbE/β⁺ thalassemia has been characterized using multiple techniques.  相似文献   

Microfluidic platform for the study of intercellular communication via soluble factor-cell and cell-cell paracrine signaling     

Matthew B. Byrne  Lisa Trump  Amit V. Desai  Lawrence B. Schook  H. Rex Gaskins  Paul J. A. Kenis 《Biomicrofluidics》2014,8(4)

Diffusion of autocrine and paracrine signaling molecules allows cells to communicate in the absence of physical contact. This chemical-based, long-range communication serves crucial roles in tissue function, activation of the immune system, and other physiological functions. Despite its importance, few in vitro methods to study cell-cell signaling through paracrine factors are available today. Here, we report the design and validation of a microfluidic platform that enables (i) soluble molecule-cell and/or (ii) cell-cell paracrine signaling. In the microfluidic platform, multiple cell populations can be introduced into parallel channels. The channels are separated by arrays of posts allowing diffusion of paracrine molecules between cell populations. A computational analysis was performed to aid design of the microfluidic platform. Specifically, it revealed that channel spacing affects both spatial and temporal distribution of signaling molecules, while the initial concentration of the signaling molecule mainly affects the concentration of the signaling molecules excreted by the cells. To validate the microfluidic platform, a model system composed of the signaling molecule lipopolysaccharide, mouse macrophages, and engineered human embryonic kidney cells was introduced into the platform. Upon diffusion from the first channel to the second channel, lipopolysaccharide activates the macrophages which begin to produce TNF-α. The TNF-α diffuses from the second channel to the third channel to stimulate the kidney cells, which express green fluorescent protein (GFP) in response. By increasing the initial lipopolysaccharide concentration an increase in fluorescent response was recorded, demonstrating the ability to quantify intercellular communication between 3D cellular constructs using the microfluidic platform reported here. Overall, these studies provide a detailed analysis on how concentration of the initial signaling molecules, spatiotemporal dynamics, and inter-channel spacing affect intercellular communication.  相似文献   

Circadian Rhythm of Serum Cortisol in Female Patients with Fibromyalgia Syndrome     

Ghizal Fatima  Siddharth Kumar Das  Abbas Ali Mahdi  Nar Singh Verma  Faizan Haider Khan  Amit Mani Kumar Tiwari  Tabrez Jafer  Baby Anjum 《Indian journal of clinical biochemistry : IJCB》2013,28(2):181-184

Fibromyalgia syndrome (FMS) patients have disturbed sleep patterns which may lead to altered circadian rhythm in serum cortisol secretion. The aim of this study was to assess circadian changes, if any, in serum cortisol levels in female patients with FMS. Cortisol levels were estimated every 6 h during 24 h period; in 40 female patients satisfying ACR criteria for FMS (Age 36.4 ± 9.9), and 40 healthy females without FMS (Age 33.8 ± 11.1). A significant difference in the night time serum cortisol level was observed among the patients and control groups (patients, 12.9 ± 9.7 controls 5.8 ± 3.0; p < 0.01). However, no significant difference was found in serum cortisol levels in patients and control groups in the morning (patients, 28.4 ± 13.2 controls, 27.6 ± 14.5; p > 0.05), afternoon (patients, 14.4 ± 5.6 controls, 14.0 ± 6.6; p > 0.05) and evening hours (patients, 10.9 ± 5.8 controls, 8.9 ± 3.6; p > 0.05). It could be concluded that there is an abnormality in circadian secretion of cortisol in female FMS patients.  相似文献   

Labor migrants’ self-empowerment via participation in a diasporic magazine: Filipinos at Manila-Tel Aviv     

Amit Kama 《亚洲交流杂志》2013,23(3):223-238

This study delves into the motivations, symbolic rewards, and experiences of Filipinos involved in the creation of a magazine catering to the Filipino migrant workers’ community in Israel. Although practices of resistance are the prevailing framework within research about diasporic media, this paper offers another perspective of the construction of a subjugated minority's sphericule. Power (or lack thereof) is not necessarily a basic force of motivation. Participation in cultural production is not perceived as a journalistic endeavor by Filipinos, but a rare and crucial opportunity to be heard, to have a voice, to win over coerced living circumstances of alienation, solitude, and hard work. In this context they are not ‘just the caregiver,’ but accomplished writers, winners of competitions, and recipients of respect. Empowerment is derived from pleasure; it is grounded in recreational gratifications and a sense of mission that have no political dimensions.  相似文献   

Ultra-sensitive hybrid diamond nanothermometer     

Chu-Feng Liu  Weng-Hang Leong  Kangwei Xia  Xi Feng  Amit Finkler  Andrej Denisenko  Jrg Wrachtrup  Quan Li  Ren-Bao Liu 《国家科学评论(英文版)》2021,8(5)

Nitrogen-vacancy (NV) centers in diamond are promising quantum sensors because of their long spin coherence time under ambient conditions. However, their spin resonances are relatively insensitive to non-magnetic parameters such as temperature. A magnetic-nanoparticle-nanodiamond hybrid thermometer, where the temperature change is converted to the magnetic field variation near the Curie temperature, were demonstrated to have enhanced temperature sensitivity () (Wang N, Liu G-Q and Leong W-H et al. Phys Rev X 2018; 8: 011042), but the sensitivity was limited by the large spectral broadening of ensemble spins in nanodiamonds. To overcome this limitation, here we show an improved design of a hybrid nanothermometer using a single NV center in a diamond nanopillar coupled with a single magnetic nanoparticle of copper-nickel alloy, and demonstrate a temperature sensitivity of . This hybrid design enables detection of 2 mK temperature changes with temporal resolution of 5 ms. The ultra-sensitive nanothermometer offers a new tool to investigate thermal processes in nanoscale systems.  相似文献