Evaporation-based microfluidic production of oil-free cell-containing hydrogel particles     

Rong Fan  Kubra Naqvi  Krishna Patel  Jun Sun  Jiandi Wan 《Biomicrofluidics》2015,9(5)

We demonstrate an evaporation-based microfluidic strategy to produce oil-free cell containing hydrogel particles. Perfluoro-n-pentane, which is used as the continuous oil phase to generate cell-containing hydrogel (Extracel) particles, is removed at an elevated temperature. Human colon cancer cells (HCT116) encapsulated in the hydrogel particles show higher viability than cells encapsulated in particles that are produced via a non-evaporative oil phase. In addition, single HCT116 cells can be cultured for a week in such particles and respond to inflammatory stimuli, highlighting the potential applications of the developed strategy for 3D cell culture, drug testing, and cell-based drug delivery.  相似文献   

Oxidant-antioxidant status in colorectal cancer patients—Before and after treatment     

Sharmila Upadhya  Subramanya Upadhya  S. Krishna Mohan  K. Vanajakshamma  Mamatha Kunder  Seema Mathias 《Indian journal of clinical biochemistry : IJCB》2004,19(2):80-83

Erythrocyte antioxidant glutathione and malondialdehyde levels in erythrocytes and plasma glutathione S-transferase levels were estimated in patients with colorectal cancer and compared to controls. Further, the patients underwent four weeks of radiotherapy with adjuvant chemotherapy. The same parameters were estimated after four weeks of radiotherapy and compared with pretreatment levels. It was observed that there was a decrease in erythrocyte glutathione and malondialdehyde levels in patients with colorectal cancer compared to controls, but not in case of GST. However, after chemoradiotherapy, there were no statistically significant differences in all the parameters studied.  相似文献   

Predicting flow induced vibration in u-bend regions of heat exchangers: an engineering solution     

Krishna P. Singh 《Journal of The Franklin Institute》1976,302(2):195-205

From the standpoint of flow induced vibrations, U-bends of tubular heat exchangers constitute structurally one of the most vulnerable regions. The U-bends possess relatively low out-of-plane frequency enabling them to extract energy from the shell stream at low flow velocities. All published correlations in the literature imply the existence of a direct relationship between the flow velocities and the incidence of large ampitude tube vibrations. Hence it is important to determine the velocity profile in the U-bend region accurately. A method to obtain an engineering solution is proposed herein which may be utilized in conjunction with the available correlations to reliably predict the possibility of vibration. Determination of the flow profile may be further utilized to improve the estimates of shellside heat transfer coefficients.  相似文献   

Synergetic Interaction of HLA-DRB1*07 Allele and TNF-Alpha − 863 C/A Single Nucleotide Polymorphism in the Susceptibility to Systemic Lupus Erythematosus     

Shiva Krishna Katkam  Liza Rajasekhar  Fathima S. D. Tasneem  Vijay Kumar Kutala 《Indian journal of clinical biochemistry : IJCB》2021,36(1):59

Systemic lupus erythematosus (SLE) is an inflammatory autoimmune disease which is characterized by dysregulation of various cytokines propagating the inflammatory processes that is responsible for tissue damage. Tumor necrosis factor alpha (TNF-α) is one of the most important immunoregulatory cytokines that has been implicated in the different autoimmune diseases including SLE. Two hundred and two patients with SLE and 318 controls were included in the study. The TNF-α gene promoter region (from − 250 to − 1000 base pairs) was analyzed by direct Sanger’s DNA sequencing method to find promoter variants associated with South Indian SLE patients. We have analyzed six TNF-α genetic polymorphisms including, − 863C/A (rs1800630), − 857C/T (rs1799724), − 806C/T (rs4248158), − 646G/A (rs4248160), − 572A/C (rs4248161) and − 308G/A (rs1800629) in both SLE patients and controls. We did not find association of TNF-α gene promoter SNPs with SLE patients. However, the − 863A (rs1800630) allele showed association with lupus nephritis phenotype in patients with SLE (OR: 1.62, 95%CI 1.04–2.53, P = 0.034). We found serum TNF-α level was significantly elevated in SLE cases as compared to control and found no association with any of the polymorphisms. The haplotype analysis revealed a significant protective association between the wild TNF-α alleles at positions − 863C, − 857C, − 806C, − 646G, − 572A and − 308G (CCCGAG) haplotype with lupus nephritis phenotype (OR 0.53, 95% CI 0.35–0.82, P = 0.004). Additionally, the TNF-α − 863 C/A (rs1800630) polymorphism and HLA-DRB1*07 haplotype showed significant differences between SLE patients and controls (OR 4.79, 95% CI 1.73–13.29, P = 0.0009). In conclusion, TNF-α − 863A allele (rs1800630) polymorphism is associated with increased risk of nephritis in South Indian SLE patients. We also found an interaction between HLA-DRB1*07 allele with TNF-α − 863 C/A promoter polymorphism giving supportive evidence for the tight linkage disequilibrium between TNF-α promoter SNPs and MHC class II DRB1 alleles.  相似文献   

Cross-Sectoral Differences in Intellectual Property Enforcement in Developing Countries: The Role of State-Industry Linkages     

Krishna P. Jayakar 《The Information Society》2013,29(2):155-169

Leading developing countries have recently introduced some reforms in their national intellectual property regimes to harmonize them with international treaties and agreements. However, major differences remain in how these laws are applied to different information industries, and how they are enforced on the ground. While intellectual property law applies to a host of information products ranging from music to computer programs, governments have selectively enforced the law in some information industries, while neglecting to extend the same protection to others. Based on the comparative institutionalist approach, this article identifies two variables--state-industry linkages and level of innovative activity--that may explain the selective enforcement of intellectual property law. Intellectual property rights were expected to be better protected in information industries with strong state-industry linkages and higher levels of domestic innovation. This expectation was tested through a comparative study of four intellectual property industries from the Asian region. The results confirmed the expectation, but also demonstrated that strong state-industry linkages may independently correlate with high levels of intellectual property protection, even in industries where the current levels of innovative activity are low.  相似文献   

Self presentation in online environments: a study of Indian Muslim matrimonial profiles     

Smeeta Mishra  Mathukutty M. Monippally  Krishna P. Jayakar 《亚洲交流杂志》2013,23(1):38-53

This study examines the self presentation practices of Indian Muslim men and women in online matrimonial advertisements. Findings indicate that while Indian Muslims are using the new medium to adhere to traditional cultural and religious values, they are also making critical adjustments to adapt to the medium itself. While Muslim men and women claimed to possess different attributes in their profiles drawing upon gender role expectations in Indian society, they were identical in their preference for light skin or what is termed as ‘fair complexion’ in Indian English. An overwhelming majority of advertisers claimed to possess desirable skin tones and body types. Few Indian Muslim profiles highlighted external manifestations of religiosity, such as praying five times a day, wearing a hijab or burqa, and observing Ramadan. Further, results of this study also illuminate the influence of South Asian culture on Islamic practice, which is evident in the mention of caste identifications in the online matrimonial profiles.  相似文献   

Affirmative action in higher education in India: targeting,catch up,and mismatch     

Veronica Frisancho  Kala Krishna 《Higher Education》2016,71(5):611-649

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Clinical utility of serum thyroglobulin in acute haemorrhage into the thyroid     

A. Kumar  D. H. Shah  U. Vijayan  S. R. Dandekar  B. A. Krishna  S. M. Sharma 《Indian journal of clinical biochemistry : IJCB》1994,9(1):19-20

Seven cases of suspected haemorrhage In pre-existing adenoma or cyst in the thyroid showed serum thyroglobulin (tg) levels phenomenonly elevated. The serum tg leveis reduced remarkably within one to three montha. During this period all patients were treated with thyroxin. It is concluded that serial serum thyroglobulin determinations may be useful in confirming the clinical suspicion of haemorrhage in the thyroid.  相似文献   

Spectrum of monoclonal gammapathies in Andhra Pradesh     

T. Malati  B. Yadagiri  D. Murali Mohan Krishna  V. Shantaram  D. Raghunadharao  K. Subbarao 《Indian journal of clinical biochemistry : IJCB》2001,16(1):52-59

In the present study, monoclonal gammapathy was identified in a total of 245 patients of plasma cell dyscrasias during period of 1987 to 2000. The monoclonal band was identified in serum by agar gel electrophoresis in all the cases and in urine in a few cases. Characterization of paraprotein (monoclonal immunoglobulin class and light chain type) was carried out by employing immunoelectrophoresis and/or immunofixation electrophoresis using heavy chain specific gamma, alpha, mu, delta and epsilon and light chain specific kappa (K), lambda (λ) antisera. Serum immunoglobulins Ig G, Ig A, and Ig M were estimated by immunoturbidometry. Serum urea, creatinine, uric acid, alkaline phosphatase, total proteins, albumin, calcium and phosphorus were estimated by using routine biochemical methods. Among the 245 cases, 73.1% monoclonal gammapathies were of secretory type and 7.3% were non-secretory. Monoclonal gammapathies were associated with 80.4% of multiple myeloma, 8.9% of solitary plasmacytoma, 4.1% of extra-medullary plasmacytoma, 3.3% of lymphoma and 2.9% of plasma cell leukemia. Classification of secretory monoclonal immunoglobulin revealed monoclonal immunoglobulin Ig G in 74%, Ig A 15% and Ig M in 2.9% cases.  相似文献   

Serum fructosamine in a random group of adult onset diabetics of Indian subpopulation     

Tangirala Malati Ph. D.  D. Murali Mohan Krishna  B. Sadasivudu 《Indian journal of clinical biochemistry : IJCB》1991,6(1):51-57

Serum fructosamine (SFRC) was estimated using single colour reading procedure in 50 normals and 160 adult onset random diabetics. Serum fructosamine levels were observed to be 1.86±0.321 m moles/L (Range 1.0 to 2.4) and 3.44±0.671 (Range 2.0 to 5.7) in normal and diabetic subjects respectively. Serum fructosamine levels as determined by the method adapted, were found to be unaffected by a transient rise in serum glucose levels during an oral glucose tolerance test. Significantly elevated SFRC concentration was also seen in diabetic groups in spite of different combinations of antidiabetic treatment. Attempts to correlate SFRC with duration and secondary complications of diabetes did show significant elevation in all the groups irrespective of duration and complications thus indicating poor glycemic control.  相似文献