Stretching of DNA confined in nanochannels with charged walls     

Chiara Manneschi  Paola Fanzio  Tapio Ala-Nissila  Elena Angeli  Luca Repetto  Giuseppe Firpo  Ugo Valbusa 《Biomicrofluidics》2014,8(6)

There is currently a growing interest in control of stretching of DNA inside nanoconfined regions due to the possibility to analyze and manipulate single biomolecules for applications such as DNA mapping and barcoding, which are based on stretching the DNA in a linear fashion. In the present work, we couple Finite Element Methods and Monte Carlo simulations in order to study the conformation of DNA molecules confined in nanofluidic channels with neutral and charged walls. We find that the electrostatic forces become more and more important when lowering the ionic strength of the solution. The influence of the nanochannel cross section geometry is also studied by evaluating the DNA elongation in square, rectangular, and triangular channels. We demonstrate that coupling electrostatically interacting walls with a triangular geometry is an efficient way to stretch DNA molecules at the scale of hundreds of nanometers. The paper reports experimental observations of λ-DNA molecules in poly(dimethylsiloxane) nanochannels filled with solutions of different ionic strength. The results are in good agreement with the theoretical predictions, confirming the crucial role of the electrostatic repulsion of the constraining walls on the molecule stretching.  相似文献   

Comparison of Freelite and N-Latex serum free light chain assays: a critical review     

Massimo Daves  Andrea Piccin  Vincenzo Roccaforte  Giuseppe Lippi 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2021,31(3)

IntroductionThe measurement of serum free light chain (FLC) represents a fundamental aspect on the assessment of patients with monoclonal gammopathies (MG). Different analytical methods for FLC have become available with the possibility to obtain different value with a substantial impact on the assessment of patients with MG. This study aimed to evaluate FLC results obtained with two different assays and how the difference value obtained can impact in the patient’s assessment.Materials and methodsNinety-three patient serum samples that underwent analysis for FLC with two different methods, Serum Freelite (The Binding Site, Birmingham, UK) and N-Latex FLC (Siemens, Marburg, Germany), were included in this retrospective study. Statistical analysis was performed to evaluate correlation, difference, and the grade of concordance between the results obtained with the two methods.ResultsSignificant statistical differences between the results obtained from the two methods were found (P < 0.05). A good correlation was found (0.99 for κ FLC, 0.95 for λ FLC, and 0.94 for the κ/λ ratio, respectively). We found a weighted kappa value of 0.65 for κ/λ ratio, 0.65 for λ FLC and 0.90 for κ FLC. A positive bias found with the Bland-Altman plot mirrors overestimation of κ FLC and κ/λ ratio with Freelite compared to N-Latex, whilst a negative bias underscores underestimation of λ FLC by Freelite compared to N-Latex.ConclusionAlthough in general the concordance between Freelite and N-Latex appears satisfactory, several discrepancies could be evidenced and consequently the two assays are not interchangeable.  相似文献   

Explorations of a Paradox in Curriculum Control: Resistance to Open-ended Science Inquiry in a School for Self-directed Learning     

J. Lawrence Bencze  Maurice Di Giuseppe 《Interchange》2006,37(4):333-361

Despite official government srt (in various jurisdictions around the world) for providing students with opportunities to construct their own knowledge within the context of formal schooling, school science systems continue to place greatest priority on teaching and learning of products of science (e.g., laws and theories), while compromising students’ opportunities to develop realistic conceptions about science and expertise for doing science. Based on qualitative data analyzed using constant comparative methods (based on constructivist grounded theory), we found this also to be the case – paradoxically – in a school belonging to the Canadian Coalition for Self-directed Learning (CCSDL). Schools in this coalition espouse, among various goals, enabling students to construct their own knowledge, in ways and directions suiting their individual needs, interests, perspectives, and abilities, in addition to gaining access to knowledge developed by society. The science department within the coalition school in this study experienced considerable difficulty realizing this goal, despite school-level administrative support for a concerted effort to reinvent itself along these lines. Factors that appeared to influence the science department’s efforts included those in each of Schwab’s (1969) educational "commonplaces;” that is, the curriculum, teachers, students, and the milieu surrounding teaching and learning. Further analysis suggests that results can be explained through reference to a Kuhnian (1962/1996) paradigm conflict – in which the school’s administrative and curricular committee and other members of the CCSDL were unsuccessful in convincing members of the science department (who, in turn, appeared to be supported by the provincial government, parents, and students) to make provisions for more student-directed, openended science inquiry. Assuming that student-led scientific inquiry continues to be an important curricular goal, efforts must continue to be made, therefore, to convince members of the mainstream paradigm that it is a worthy goal.  相似文献   

Cycling shoe aerodynamics     

Giuseppe Gibertini  Donato Grassi  Carlo Macchi  Giuseppe De Bortoli 《Sports Engineering》2010,12(3):155-161

The present paper describes the activity carried out to investigate the aerodynamic effects of cycling shoes for time trial competitions. This subject has not been widely studied but can be important for an accurate aerodynamic optimisation of a time trial cyclist. The study was carried out by means of wind tunnel testing: an appropriate test setup and an appropriate test procedure (based on “effective angle of attack approach”) were developed in order to produce realistic test conditions. The developed testing procedure was applied to two different shoe models, differently fastened. Furthermore, an important point was the investigation of the overshoe effect. The results showed that the power required to overcome the shoe’s drag is almost a tenth of the total power and that differences between the shoes can affect the cyclist’s performance.  相似文献   

Propagation of projective synchronization in a series connection of chaotic systems     

Giuseppe Grassi ^{Author Vitae} 《Journal of The Franklin Institute》2010,347(2):438-451

Projective synchronization is a type of chaos synchronization where the response system states are scaled replicas of the drive system states. This paper deals with the propagation of projective synchronization in a series connection of N chaotic discrete-time drive systems and N response systems. By exploiting an observer-based approach, the paper demonstrates that dead-beat projective synchronization (i.e., exact synchronization in finite time for any scaling factor) is achieved between the nth drive and nth response systems. In particular, it is shown that projective synchronization starts from the innermost (Nth) drive-response system pair and propagates toward the outermost (first) drive-response system pair. Only a single scalar synchronizing signal connects the cascaded drive and response systems. Finally, an example illustrates the propagation of different types of chaos synchronization in a series connection consisting of a Gingerbreadman map, a third order hyperchaotic Henon map and a Lozi map.  相似文献