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Danny Bavli Elishai Ezra Daniel Kitsberg Margarita Vosk-Artzi Shashi K. Murthy Yaakov Nahmias 《Biomicrofluidics》2016,10(2)
Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotin-labeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α-Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation. 相似文献
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Vuokko Kohtamäki 《Higher Education Quarterly》2011,65(2):164-185
Numerous studies have addressed how state authorities and public funding bodies control, regulate, steer, supervise or oversee higher education institutions. There are fewer studies on how higher education institutions respond to the actions of the mentioned authorities. States have aimed at increasing the autonomy of higher education institutions. At the same time institutions are expected to generate social benefits and economic growth. There is an endless tension between a policy that will strengthen the autonomy and simultaneously retain a space for state steering. The Finnish polytechnic and university sectors have very different origins from which their financial autonomy takes its shape. This article sheds light on how six Finnish polytechnics aspire to enhance their financial autonomy. The polytechnics operate under two separate steering systems, the state and local owners, and both of these actors exercise their own steering. This article shows that polytechnics are willing and able to enhance their financial autonomy. They calculate their advantages and habitually engage with the Ministry of Education or their owners depending on the case in question. The financial autonomy of polytechnics does not culminate in a form of diversified funding sources, but as balancing between the two masters on whose resources they are heavily dependent. 相似文献