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81.
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83.
Luciferase from firefly lantern extract was immobilized on CNBr-activated Sepharose 4B. The kinetic properties of immobilized
luciferase were extensively studied. TheK
m′ for D-luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose-bound enzyme were 7.8 and 25°C respectively.
A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak
light intensity was linear with respect to ATP concentration in range of 10−9–10−5 mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal
oxygen environments. 相似文献
84.
Patrick J. Herling B. Tanya Mohseni Derek C. Hill Stacy Chelf Jeffrey A. Rickert Jonathan T. Leo Natalie R. Langley 《Anatomical sciences education》2017,10(3):215-223
Lincoln Memorial University‐DeBusk College of Osteopathic Medicine (LMU‐DCOM) offers an optional three‐week summer Anatomy Boot Camp course (ABC) to facilitate students' transition into medical school and promote retention of anatomy subject matter. The pre‐matriculation program is a supplemental instruction course that utilizes a small group learning format. Boot camp instruction is led by teaching assistants and two anatomy professors. Enrollees gain early exposure to Medical Gross Anatomy (MGA) course subject matter, which is taught in the fall semester, and learn study skills necessary to excel in medical school. No grade is assigned for the course, therefore participants can study without the fear of potentially affecting grades. This study evaluates the effectiveness of the LMU‐DCOM ABC course using data from four consecutive summers. Independent two‐sample t‐tests were used to compare ABC to non‐ABC students for the following variables: incoming grade point average (GPA) and Medical College Admission Test® (MCAT®) scores, MGA written and laboratory practical examination grades, and final MGA course grade. Additionally, a 26‐question survey was administered to 2012–2014 boot camp participants. There were no significant differences in incoming GPA and MCAT scores. However, boot campers scored significantly higher on the first two lecture and laboratory examinations (P < 0.05) for each year of the study. Thereafter scores varied less, suggesting a faster head start for boot camp participants. Mean MGA final grade was on average 3% higher for the boot camp cohort. The survey feedback supports that the ABC course assists with the academic and social transition into medical school. Anat Sci Educ 10: 215–223. © 2016 American Association of Anatomists. 相似文献
85.
William J. Meyer Patrick Morrison Anayansi Lombardero Kelsey Swingle Duncan G. Campbell 《Journal of College Student Psychotherapy》2016,30(3):197-205
Unwillingness to share depression experiences with primary care physicians contributes to the undertreatment of depression. This project examined college students’ reasons for depression nondisclosure to primary care providers (PCPs). Undergraduate participants read a vignette describing someone with depression and completed measures of disclosure barriers. Analyses indicated 26% of respondents would NOT disclose depression symptoms to PCPs. Reasons for nondisclosure included difficulty initiating the depression conversation, medication aversion, and fear of referral to a psychologist. Also, anger toward the vignette character varied with disclosure willingness, suggesting that nondisclosers feel more stigma than disclosers and are less likely to engage in psychotherapy. Because engagement in pharmacological and/or psychotherapeutic treatment requires identification of depression in healthcare settings, increased understanding of disclosure barriers could increase students’ access to depression treatment. 相似文献
86.
87.
Lina?KashyapEmail author 《International journal for the advancement of counseling》2004,26(4):337-339
In 2001, for the first time in the history of the International Association for Counselling, International Conference was held in a developing Asian country–India, with the theme Families in Transition–Counselling in Transition–Meeting the Challenges Ahead. This special issue contains some of the presentations made by Indian participants during this conference which best reflect the challenges and diversities of counseling efforts with families in India. The first article was specially written for this issue in order to give an international relationship of this journal an in depth contextual understanding of Indian families in transition and the challenges they pose to the contemporary Indian counselor. Two articles are reflected to counseling families affected by socio-politial and economic changes. Five authors have written on counseling related to physical and mental disability/addiction and last two articles are reflected to authors experiences as family counselors. 相似文献
88.
Patrick W. Thompson 《Educational Studies in Mathematics》1993,25(3):165-208
Six fifth-grade children participated in a four-day teaching experiment on complex additively-structured problems, which was followed by in-depth interviews of individual children. The teaching experiment was meant to investigate children's difficulties in holding in mind at once situations in which one or more items played multiple roles. Two important difficulties were identified: (1) distinguishing between difference as the result of subtracting and difference as the amount by which one quantity exceeded another; and (2) indirect evaluation of an additive comparison. Sources of these difficulties, along with pedagogical and curricular recommendations for addressing them, are discussed.Research reported in this paper was supported by National Science Foundation Grants NO. MDR 89-50311 and 90-96275, and by a grant of equipment from Apple Computer, Inc., Office of External Research. Any conclusions or recommendations stated here are those of the author and do not necessarily reflect official positions of NSF or Apple Computer. 相似文献
89.
Kinetic knowledge is of great importance in achieving good control of the pyrolysis and gasification process and optimising
system design. An overall kinetic pyrolysis scheme is therefore addressed here. The kinetic modelling incorporates the following
basic steps: the degradation of the virgin biomass materials into primary products (tar, gas and semi-char), the decomposition
of primary tar into secondary products and the continuous interaction between primary gas and char. The last step is disregarded
completely by models in the literature. Analysis and comparison of predicted results from different kinetic schemes and experimental
data on our fixed bed pyrolyser yielded very positive evidence to support our kinetic scheme.
Project (No. 2001CB409600) Supported by the National Key Basic Research Program (973) of China 相似文献
90.
普通话二字组词语不同的声调组合方式 ,使前后位声调产生不同的动态变化形式。前位声调的动态变化明显表现在上声调变为低降调和中升调上 ;后位声调的动态变化则表现在调值的明显改变上。后位声调的动态变化更为普遍 ,应该引起足够的重视 相似文献