Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping     

Bj?rn Hammarstr?m  Hong Yan  Johan Nilsson  Simon Ekstr?m 《Biomicrofluidics》2013,7(2)

Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 μm antibody covered beads is presented. The acoustic trapping is done in 200 × 2000 μm² glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples.  相似文献   

Changes in the perception of action possibilities while climbing to fatigue on a climbing wall     

J. R. Pijpers  Ra?ul R. D. Oudejans  Frank C. Bakker 《Journal of sports sciences》2013,31(1):97-110

Abstract

In two experiments we examined changes in the perception of action possibilities as a function of exertion. In Experiment 1, participants repeatedly climbed on a climbing wall in a series of trials that progressively increased in number to 10 trials, resulting in increased exertion. Before and during climbing, the participants judged their maximum reaching height and perceived exertion. On a separate day, participants climbed another 10 trials while performing actual maximum reaches. Higher perceived exertion was associated with decreases in perceived maximum reach while the actual reaches did not decrease. However, the perceptual changes occurred early during task execution when the participants were not yet fatigued. When exertion set in, neither perceived nor actual maximum reaching appeared to be affected. In Experiment 2, we included exhaustion trials. The findings replicated the early changes in perception observed in Experiment 1, which may be explained by hands-on experience with the task. Furthermore, while climbing to exhaustion, perceptual judgements largely changed in keeping with changes in the actual maximum reach. Thus, there appeared to be a functional relationship between participants' actual action capabilities, rather than their state of physical fatigue per se, and perceived action possibilities.  相似文献   

一种企业研发团队复杂网络平台体系架构     

李建勋  李维乾  郭莲丽  邓朝晖  刘斯 《现代情报》2013,33(10):128-131

针对现有复杂网络软件难以支撑企业研发团队分析的问题,建立一个灵活、可扩展的关系网络的展示环境和可视化分析环境,提炼出研发团队复杂网络映射方案、表示方案,通过特征指标关联反映研发团队复杂网络的内在本质,形成以行为为准则的复杂网络动态演化机制,为企业研发团队的复杂网络分析提供一个具有复杂网络建模、分析展示系统、指标特征关联、网络动态演化4个层面的专业平台架构。  相似文献   

Knowledge management practices and performance: are they truly linked?     

Sharmila?JayasingamEmail author  Mahfooz A?Ansari  T?Ramayah  Muhamad?Jantan 《知识管理研究与实践》2013,11(3):255-264

Knowledge management (KM) has often been claimed to be an essential ingredient in building competitive advantage. Yet, KM adoption is relatively slow, especially in the Malaysian context. Most organizations are unsure if the promised performance improvement is just a passing fad. This study intends to provide empirical verifications to support the link between KM practices and performance outcomes for organizations. Responses from 180 knowledge-based organizations were analysed. The analysis indicated that knowledge acquisition and knowledge utilization positively influenced strategic and operational improvement in organizations, whereas the positive effect of knowledge dissemination was only evident in the case of strategic improvement. Organization size had some interesting moderating impact on the tested relationships.  相似文献   

A citation and co-citation analysis of 10 years of KM theory and practices     

Christian?Walter  Vincent?RibièreEmail author 《知识管理研究与实践》2013,11(3):221-229

In a previous issue of Knowledge Management Research & Practice (KMRP), we analysed the content and keywords of all articles published in the first decade of KMRP. With this article, we extend our preliminary analysis to the citation and co-citations made by these articles. The study covers all the 256 articles published. The most cited article was A dynamic theory of organisational knowledge creation by Nonaka. The most cited KMRP article was by Nonaka and Toyama: The knowledge-creating theory revisited: knowledge creation as a synthesizing process. The co-citation analysis of the 100 most cited articles in KMRP publications showed that four groups of topics emerged, one around communities and situated learning, the second group around networks, knowledge transfer and research methods, a third group around the foundations of knowledge management and a fourth group around intellectual capital.  相似文献   

Deciding to use an enterprise wiki: the role of social institutions and scripts     

Paul?JacksonEmail author  Jane?Klobas 《知识管理研究与实践》2013,11(4):323-333

  相似文献   

Embedding with multiple knowledge sources to improve innovation performance: the learning experience of Motorola in Brazil     

Paulo N?FigueiredoEmail author 《知识管理研究与实践》2013,11(4):361-373

This article explores how a subsidiary of a multi-national enterprise (MNE) in an emerging economy embeds with multiple knowledge sources, both in its corporate network (internal) and in the host country (external), to create the capability to undertake innovative activities. Drawing on first-hand and intra-firm field evidence over the lifetime of a subsidiary in the information and communications technology industry in Brazil (1996–2009) the article finds that: (1) Within 10 years, the case subsidiary attained advanced, near-world leading innovation performance in three technological functions: project management, software engineering and product and solutions; (2) This was an outcome of how the firm simultaneously engaged in knowledge-intensive linkages based on increased frequency of use and improved quality over time, with specific internal and external counterparts; (3) However, some counterparts and linkages were more effective than others in contributing to the subsidiary's innovation performance. Drawing on a novel approach that examines the relationship between dual embeddedness and innovative performance, this article extends our understanding of embeddedness as part of knowledge-seeking strategies of MNE-subsidiaries. It also provides new insights to deepen the analysis of the nuances of subsidiaries’ multiple embeddedness, especially in emerging economies.  相似文献   

Organisational ‘know-where’ and ‘know-when’: re-framing configurations and distributions of knowledge in organisations     

Andrew?AthertonEmail author 《知识管理研究与实践》2013,11(4):410-421

This paper explores varying configurations of knowledge in organisations. A multi-dimensional framework of distinctive forms of knowledge is used to highlight different patterns of organisational knowledge, demonstrating their heterogeneity and variability – over time, in terms of depth of expertise, and their distribution within and between organisations. Common knowledge that is shared across an organisation accounts for only a small proportion of organisational knowledge, indicating that strategies to exploit this resource for competitive advantage will be constrained unless mechanisms are developed that enable access to and transfer of knowledge that is not yet shared or common.  相似文献   

The effects of transport by pneumatic tube system on blood cell count,erythrocyte sedimentation and coagulation tests     

Fatma Emel Ko?ak  Mustafa Y?ntem  ?zlem Yücel  Mustafa ?ilo  ?zlem Gen?  Ayfer Meral 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2013,23(2):206-210

Introduction:

Today, the pneumatic tube transport system (PTS) is used frequently because of its advantages related to timing and speed. However, the impact of various types of PTS on blood components is unknown. The aim of this study was to examine the influence of PTS on the quality of routine blood cell counts, erythrocyte sedimentation, and certain blood coagulation tests.

Materials and methods:

Paired blood samples were obtained from each of 45 human volunteers and evaluated by blood cell count, erythrocyte sedimentation, and several coagulation tests, including prothrombin time (PT) and activated partial thromboplastin time (aPTT). Blood samples were divided into 2 groups: Samples from group 1 were transported to the laboratory via the PTS, and samples from group 2 were transported to the laboratory manually. Both groups were evaluated immediately by the tests listed above.

Results:

The blood sample test results from groups 1 and 2 were evaluated and compared. No statistically significant differences were observed (P = 0.069–0.977).

Conclusion:

The PTS yielded no observable effects on blood cell counts, erythrocyte sedimentation, or PT and aPTT test results. We concluded that the PTS can be used to transport blood samples and yield reliable results for blood cell counts, erythrocyte sedimentation, and several coagulation tests.  相似文献   

Croatian Society of Medical Biochemistry and Laboratory Medicine: national recommendations for venous blood sampling     

Nora Nikolac  Vesna ?upak-Smol?i?  Ana-Maria ?imundi?  Ivana ?elap 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2013,23(3):242-254

Phlebotomy is one of the most complex medical procedures in the diagnosis, management and treatment of patients in healthcare. Since laboratory test results are the basis for a large proportion (60–80%) of medical decisions, any error in the phlebotomy process could have serious consequences. In order to minimize the possibility of errors, phlebotomy procedures should be standardised, well-documented and written instructions should be available at every workstation. Croatia is one of the few European countries that have national guidelines for phlebotomy, besides the universally used CLSI (Clinical Laboratory Standards Institute) H3-A6 Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture; approved Standard-Sixth Edition (CLSI, 2007) and WHO (World Health Organization) guidelines on drawing blood: best practices in phlebotomy (WHO, 2010). However, the growing body of evidence in importance of preanalytical phase management resulted in a need for evidence based revision and expansion of existing recommendations.The Croatian Society for Medical Biochemistry and Laboratory Medicine, Working Group for the Preanalytical Phase issued this recommendation. This document is based on the CLSI guideline H3-A6, with significant differences and additional information.  相似文献