Microfluidic chip for fast bioassays��evaluation of binding parameters     

Jakub ?těpánek  Michal P?ibyl  Dalimil ?nita  Milo? Marek 《Biomicrofluidics》2007,1(2)

A seven channel polystyrene (PS) microchip has been constructed using a micromilling machine and a high-temperature assembling. Protein A (PA) has been immobilized by a passive sorption on the microchannel walls. Two bioaffinity assays with human immunoglobulin G (hIgG) as a ligand have been carried out. (i) PA as the receptor and fluorescently labeled hIgG (FITC-hIgG) as the ligand, (ii) PA as the receptor with hIgG as the quantified ligand and fluorescently labeled goat anti-human IgG (FITC-gIgG) as the secondary ligand. One incubation step of the assays took only 5 min instead of hours typical for enzyme-linked immunosorbent assay applications. Calibration curves of the dependence of a fluorescence signal on the hIgG concentration in a sample have been obtained in one step due to a parallel arrangement of microchannels. A mathematical model of the PA-FITC-hIgG complex formation in the chip has been developed. The values of the kinetic constant of the PA-FITC-hIgG binding (k_on=5.5 m³ mol⁻¹ s⁻¹) and the equilibrium dissociation constant of the formed complex (K_d≤3×10⁻⁶ mol m⁻³) have been obtained by fitting to experimental data. The proposed microchip enables fast evaluation of kinetic and equilibrium constants of ligand-receptor bioaffinity pairs and the ligand quantification. As the use of microfluidic chips for immunoassays is often limited by price, we used procedures and chemicals that allow for an inexpensive construction and operation of the microdevice, e.g., temperature assembling as a fabrication technique, detection via an ordinary digital camera, nonspecific polystyrene as a substrate, passive sorption of biomolecules as an immobilization technique, etc.  相似文献   

Does early child negative emotionality moderate the association between maternal stimulation and academic readiness and achievement?     

Ophélie A. Collet  Massimiliano Orri  Cédric Galéra  Laura Pryor  Michel Boivin  Richard Tremblay  Sylvana Côté 《Child development》2024,95(3):948-961

We investigated whether child temperament (negative emotionality, 5 months) moderated the association between maternal stimulation (5 months–2½ years) and academic readiness and achievement (vocabulary, mathematics, and reading). We applied structural equation modeling to the data from the Quebec Longitudinal Study of Child Development (N = 1121–1448; mostly Whites; 47% girls). Compared to children with low negative emotionality, those with high negative emotionality had higher levels of academic readiness (6 years) and mathematics achievement (7 years) when exposed to high levels of maternal stimulation (β = 3.17, p < .01 and β = 2.91, p < .01, respectively). The results support the differential susceptibility model whereby highly emotionally negative children were more susceptible to the influences of low and high levels of maternal stimulation in academic readiness and mathematics achievement's developments.  相似文献   

The role of the referee in physical education lessons: student experience and motivation     

David Adé  Caroline Ganière  Benoît Louvet 《Physical Education & Sport Pedagogy》2018,23(4):418-430

Introduction: Social roles in physical education (PE) classes have been much studied, especially mentoring and coaching roles. The studies have shown that mentoring and coaching are beneficial not only for motor learning, but also for methodological and social learning. To our knowledge, the role of the student referee in PE lessons has never been specifically studied. Yet refereeing is essential in many sports, including team sports, and provides an experience of responsibility that many teachers want to offer their students. Encouraging students to take on this role can nevertheless be difficult.

Objective: The objective of the present study was to gain access to students’ lived experiences as referees in order to determine their strategies for being effective. We particularly wanted to determine which concerns organized their activity so that we could identify a refereeing typology that would be useful for PE teaching. Our study is original in that we did not rely exclusively on experiential data to understand student refereeing activity. We also collected data on the students’ motivation in order to better understand their experiences. For this purpose, the study was conducted within the methodological framework of course-of-action theory and self-determination theory.

Method: Seventy-four students from three classes in the third year of middle school (about 13 years old) participated in the study. Among them, four (two girls and two boys and not experts in the sports in which they were going to referee) had volunteered to be filmed and to participate in self-confrontation interviews. The other students completed two questionnaires to provide information on their motivation for refereeing. The situations studied were basketball and handball matches held at the end of the lessons.

Two categories of data were collected: qualitative and quantitative. The qualitative data were based on audiovisual recordings of the students as they refereed matches and verbalization data from self-confrontation interviews; these data were used to document the students’ courses of experience during the activity period under study. The quantitative data were collected using two questionnaires, one to assess the determinants of motivation and the other to assess self-determined motivation.

Results and discussion: The qualitative analysis highlighted three typical student involvements: fulfilling the role of referee, getting help, and occasionally dropping out of the role. The quantitative analysis revealed that the students in the social role of referee mainly expressed amotivation, external regulation, and intrinsic motivation toward knowledge and accomplishment.

The results are discussed around two major points: (1) the students’ strategies of alternation from which their refereeing activity emerged and (2) proposals for PE teacher interventions.  相似文献   

The existence of dyslexia without severe literacy problems     

T.?R.?MilesEmail author  T.?J.?Wheeler  M.?N.?Haslum 《Annals of dyslexia》2003,53(1):340-354

This paper is concerned with those who display positive indicators on tests for dyslexia but seemingly do not have any severe literacy problems. A case study is presented by way of illustration. On the basis of data from the British Births Cohort Study (12,905 10-year-old children), it was found that some seemingly normal achievers who showed signs of dyslexia (N=422) obtained different results on five measures associated with dyslexia than did other normal achievers without such signs (N=4,998). The measures used were: underachievement at word recognition; and underachievement at spelling, reading comprehension, pseudoword reading, and mathematics. The proportion of boys to girls in the groups was also noted. It is suggested that these were cases of mild dyslexia, who, on the basis simply of reading and spelling tests, might escape detection. The consequences for the concept of dyslexia are discussed, and it is suggested that the needs of dyslexics with only mild literacy problems should not be overlooked.  相似文献   

The translucent cadaver: An evaluation of the use of full body digital x-ray images and drawings in surface anatomy education     

Kotzé SH  Mole CG  Greyling LM 《Anatomical sciences education》2012,5(5):287-294

It has been noted by staff at the Faculty of Health Sciences, Stellenbosch University that medical students neglect the study of surface anatomy during dissection. This study reports on the novel use of Lodox® Statscan® images in anatomical education, particularly the teaching of surface anatomy. Full body digital X‐ray images (Lodox Statscan) of each cadaver (n = 40) were provided to second year medical students. During dissection students were asked to visualize landmarks, organs, and structures on the digital X‐ray and their cadaver, as well as palpate these landmarks and structures on themselves, their colleagues, and the cadaver. To stimulate student engagement with surface anatomy, dissection groups were required to draw both the normal and actual position of organs on a laminated image provided. The accuracy of the drawings was subsequently assessed and students were further assessed by means of practical identification tests. In addition, students were asked to complete an anonymous questionnaire. A response rate of 79% was obtained for the student questionnaire. From the questionnaire it was gathered that students found the digital X‐ray images beneficial for viewing most systems' organs, except for the pelvic organs. Although it appears that students still struggle with the study of surface anatomy, most students believed that the digital X‐rays were beneficial to their studies and supported their continued use in the future. Anat Sci Educ. © 2012 American Association of Anatomists.  相似文献   

The multilevel impact of transformational leadership on teacher commitment: Cognitive and motivational pathways     

Xavier Dumay  Benoît Galand 《British Educational Research Journal》2012,38(5):703-729

A growing body of research indicates that transformational leadership affects teachers’ commitment to their school. The present study aims to investigate the processes explaining this effect at the organisational level. Using a sample of 660 teachers within 50 primary French‐speaking Belgian schools, the authors test a model hypothesising that the impact of the school principal's transformational leadership (as an organisational‐level construct) on teacher commitment to school is mediated by school culture strength (cognitive pathway) and teacher collective efficacy beliefs (motivational pathway). Results of multilevel analyses largely support the theoretical model, but show that schools have a limited impact on teacher commitment.  相似文献   

The role of emotions in student teachers’ professional identity     

Inge Timoštšuk  Aino Ugaste 《欧洲师范教育杂志》2012,35(4):421-433

This paper presents findings of a qualitative interview study of the role of emotions in the professional identity of student teachers. Strong positive and negative emotions (mostly related to pupils and supervisors) were expressed about personal teaching experiences. The results confirm that emotions play an important role in social learning and, therefore, influence the development of professional identity. The two most important findings were that negative emotions exercised the strongest influence and that supervisors neglected the role of positive emotions as a support for learning. The study supports the concept that it is important for teacher educators to help student teachers understand their emotional experiences (focusing foremost on positive factors in their experience) and to develop the ability to express their own emotions in addition to understanding the emotions of others.  相似文献   

Improving information retrieval using Medical Subject Headings Concepts: a test case on rare and chronic diseases     

SJ Darmoni  LF Soualmia  C Letord  MC Jaulent  N Griffon  B Thirion  A Névéol 《Journal of the Medical Library Association : JMLA》2012,100(3):176-183

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Online discussion compensates for suboptimal timing of supportive information presentation in a digitally supported learning environment     

Omid Noroozi  Maria C. Busstra  Martin Mulder  Harm J. A. Biemans  Hilde Tobi  Anouk Geelen  Pieter van’t Veer  Mohammad Chizari 《Educational technology research and development : ETR & D》2012,60(2):193-221

This study used a sequential set-up to investigate the consecutive effects of timing of supportive information presentation (information before vs. information during the learning task clusters) in interactive digital learning materials (IDLMs) and type of collaboration (personal discussion vs. online discussion) in computer-supported collaborative learning (CSCL) on student knowledge construction. Students (N = 87) were first randomly assigned to the two information presentation conditions to work individually on a case-based assignment in IDLM. Students who received information during learning task clusters tended to show better results on knowledge construction than those who received information only before each cluster. The students within the two separate information presentation conditions were then randomly assigned to pairs to discuss the outcomes of their assignments under either the personal discussion or online discussion condition in CSCL. When supportive information had been presented before each learning task cluster, online discussion led to better results than personal discussion. When supportive information had been presented during the learning task clusters, however, the online and personal discussion conditions had no differential effect on knowledge construction. Online discussion in CSCL appeared to compensate for suboptimal timing of presentation of supportive information before the learning task clusters in IDLM.  相似文献   

Talking about death: Implementing peer discussion as a coping mechanism to overcome fears about dissection,death, and dying     

Sanet Henriët Kotzé  Calvin Gerald Mole 《Anatomical sciences education》2013,6(3):191-198

Many studies have reported on the perceptions of medical students toward dissection. It is important to understand the feelings and symptoms experienced during dissection so that they can be adequately handled. Prior to dissection, first year students are given lectures on aspects of dissection, death and dying, and death rituals in various cultures. Two separate questionnaires, one given during the first week of dissection and another given one month into the program were then completed anonymously by dissection groups. The questions were designed to be open‐ended, thereby encouraging group discussion amongst students. The questionnaires were used to determine the perception of students to dissection and to discover if these perceptions change during the dissection program. The first questionnaire revealed that students do experience fears and anxiety prior to and at the beginning of dissection; however, most of these fears dissipated by the time of the second questionnaire. One month into dissection students cited talking to peers as their main coping mechanism and fewer students mentioned emotional detachment from their cadaver as a coping mechanism, as was the case in the first questionnaire. Dissection was perceived as a positive experience by our student cohort and most students cited the main advantage of dissection as the ability to visualize organs in three dimensions. The comprehensive answers received from the students indicated that thorough discussion of feelings amongst peers occurred, introducing students to an important coping mechanism at an early stage of their learning. Anat Sci Educ. © 2013 American Association of Anatomists.  相似文献