亨廷顿舞蹈症、氧化应激与胶质细胞     

高岩  楚世峰  陈乃宏 《河北北方学院学报(医学版)》2014,(3):22-30

氧化应激的产生与胞浆内活性氧簇的升高密切相关,过多生成的活性氧簇能够造成脂质过氧化,损伤蛋白质甚至DNA,而这些物质的受损与疾病发生相关,尤其是神经退行性疾病.神经退行性疾病经常被描述为进行性的神经元丢失,伴随神经元或者胶质细胞中出现标志性寡聚体.在神经退行性疾病中,胶质细胞可能以一种抗氧化应激的方式在神经元功能维持以及生存上发挥重要作用.反之,神经元也能够通过不同途径反馈调节胶质细胞.该文主要探讨在亨廷顿舞蹈症中胶质细胞的特点和功能,阐明氧化应激与亨廷顿舞蹈症的关系,概括这三者之间的关系进而论证胶质细胞在亨廷顿舞蹈症中的抗氧化应激保护作用.  相似文献   

尼克酰胺与exendin-4诱导胰腺干细胞向β细胞定向分化研究     

解谦  ;刘利萍  ;普鸿丽  ;李宝园 《雁北师范学院学报》2014,(3):37-40

目的体外分离胰腺干细胞,选择最佳培养条件探索胰腺干细胞在体外环境下向β细胞的分化情况.方法取SD大鼠胰腺组织,胶原酶消化,密度梯度离心获得纯化的胰腺导管上皮细胞.采用分步诱导法诱导胰腺导管上皮细胞向胰岛β细胞分化;用胰岛素释放实验检测胰岛功能,免疫荧光法检测nestin,PDX-1,CK-19,CK-20胰岛素及胰高血糖素等的表达.结果胰腺消化培养6～12h,可看到胰腺导管上皮细胞贴壁生长,通过4步诱导培养,nestin阳性细胞快速生长,并可分泌胰岛素.结论体外分离的成人胰岛前体细胞,在诱导因子的作用下,胰腺干细胞可定向分化为β细胞,有望应用于糖尿病的治疗.  相似文献   

Evaluation of Luteolin in the Prevention of N-nitrosodiethylamine-induced Hepatocellular Carcinoma Using Animal Model System     

K. Balamurugan  J. Karthikeyan 《Indian journal of clinical biochemistry : IJCB》2012,27(2):157-163

Hepatocellular carcinoma (HCC) is one of the commonest tumors worldwide. The treatment of HCC is vital for disease diagnosis and prognosis, as the liver is the most important organ controlling metabolic functions. Now-a-days, western folklore medicines are largely dependent on the phyto compounds which are highly effective in therapy and with low side effects. Luteolin is a flavonoid (3,4,5,7-Tetrahydro flavones) possess anti-inflammatory, anticancer and anti allergic property. The present study evaluates the efficacy of luteolin against N-nitrosodiethylamine (DEN) induced HCC in albino rats. In the highlight of the above, luteolin was evaluated for its efficacy against DEN induced HCC in male Wistar albino rats. The Biochemical parameters such as tissue damaging enzymes viz., AST, ALP, LDH and γ-GT, enzymatic antioxidants viz., SOD, CAT, GSH and GPx and histopathological changes have been estimated. The tissue damaging enzymes were found to be high in DEN alone treated group whereas the enzymatic antioxidants decreased destructively. Severe lesions and cirrhosis were observed in the toxin (DEN alone) treated group. The luteolin treated DEN group altered the tissue damaging enzymes and the enzymatic antioxidants. The damaged lesion in the histoarchitecture of DEN treated rat liver was almost completely restored. Finally this study strongly demonstrates that luteolin has potent curative property against HCC in albino rats.  相似文献   

Clinical implications of exercise immunology     

David C. Nieman 《运动与健康科学(英文)》2012,1(1):12-17

Maintaining leanness and a physically active lifestyle during adulthood reduces systemic inflammation, an underlying factor in multiple chronic diseases. The anti-inflammatory influence of near-daily physical activity in lowering C-reactive protein, total blood leukocytes, interleukin-6, and other inflammatory cytokines may play a key role in lowering risk of cardiovascular disease, certain types of cancer, type 2 diabetes, sarcopenia, and dementia. Moderate exercise training causes favorable perturbations in immunity and a reduction in incidence of upper respiratory tract infection (URTI). During each bout of moderate exercise, an enhanced recirculation of immunoglobulins, neutrophils, and natural killer cells occurs that persists for up to 3-h post-exercise. This exercise-induced surge in immune cells from the innate immune system is transient but improves overall surveillance against pathogens. As moderate exercise continues on a near-daily basis for 12–15 weeks, the number of symptoms days with URTI is decreased 25%–50% compared to randomized sedentary controls. Epidemiologic and animal studies support this inverse relationship between URTI risk and increased physical activity.  相似文献   

Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment     

Kang-ting Ji  Jun-de Chai  Cheng Xing  Jin-liang Nan  Peng-lin Yang  Ji-fei Tang 《Journal of Zhejiang University. Science. B》2010,11(8):618-626

In this study,we examined the protective effects of Danshen both on endothelial progenitor cells(EPCs) in patients with hypercholesterolemia and on in-vitro EPCs of healthy volunteers.In the clinical study,we randomly divided 24 subjects with hypercholesterolemia into two groups(the control group and the Danshen-treated group).At the end of two weeks of treatment,the EPC cellular functions of both groups were tested.The results indicated that,compared to the control group,EPCs in the Danshen-treated group showed significantly better cellular functions,which was manifested in the cloning number,the proliferation capacity,the number of EPC adhesions,and cell migration.In the subsequent in-vitro experiments,EPCs were treated with vehicle,oxidized low-density lipoprotein(Ox-LDL,100 μg/ml),or Ox-LDL(100 μg/ml) plus different concentrations of Danshen(Danshensu 2,10,or 50 μg/ml,respectively) for 24 h.The results showed that Danshen treatments can prevent the detrimental effects of Ox-LDL on EPC cellular functions measured by proliferation capacity(0.24±0.08,0.37±0.11,0.30±0.04 vs.0.13±0.02,P0.05,P0.01,and P0.01,respectively),and adhesion ability(63.00±11.60,70.00±10.80,85.50±11.41 vs.40.50±6.85,all P0.01).Compared to the group treated with Ox-LDL alone,Danshen treatment significantly decreased the lipid peroxidation end product malondialdehyde(MDA) [(4.34±0.54),(3.98±0.47),(3.46±0.31) vs.(5.57±0.64) nmol/ml,all P0.01],increased the production of superoxide dismutase(SOD) [(29.74±0.71),(31.09±0.83),(30.41±0.65) vs.(14.76±3.99) U/ml,all P0.01],and lowered the expression of interleukin-6(IL-6) [(24.62±7.69),(27.04±3.14),(33.38±18.86) vs.(230.67±33.53) pg/ml,all P0.01] and tumor necrosis factor-α(TNF-α) [(41.72±6.10),(17.02±6.82),(3.73±2.26) vs.(228.71±41.53) pg/ml,all P0.01] in Ox-LDL treated EPCs.These results suggest that Danshen may exert a protective effect through its antioxidant and anti-inflammatory features.  相似文献   

Metachronous contralateral testicular and bilateral adrenal metastasis of chromophobe renal cell carcinoma: a case report and review of the literature     

Hai-yang Wu  Li-wei Xu  You-yun Zhang  Yan-lan Yu  Xin-de Li  Gong-hui Li 《Journal of Zhejiang University. Science. B》2010,11(5):386-389

Chromophobe renal cell carcinoma(ChRCC) metastatic to the testis has not,to the best of our knowledge,been reported in the literature.Nor have there been reports of delayed bilateral adrenal metastasis of ChRCC.Here we report a case of metachronous contralateral testicular and bilateral adrenal metastasis of ChRCC in a 70-year-old man who underwent right radical nephrectomy for RCC six years ago.He was admitted to the hospital because of left intrascrotal enlargement of two-month duration.Ultrasonography re...  相似文献   

Comparative features of colorectal and gastric cancers with microsatellite instability in Chinese patients     

Yan-qin Huang  Ying Yuan  Wei-ting Ge  Han-guang Hu  Su-zhan Zhang  Shu Zheng 《Journal of Zhejiang University. Science. B》2010,11(9):647-653

Objective  

The purpose of this study was to determine the unique and universal features of microsatellite instability-high (MSI-H) colorectal cancer (CRC) and MSI-H gastric cancer (GC) in the Chinese population.  相似文献   

Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells     

Wei Wang  Li Wang  Xin-xiu Li  Xia Chen  Hai-yan Zhang  Yu He  Jing-jing Wang  Yong-yan Zhao  Bao-le Zhang  Yin-xue Xu 《Journal of Zhejiang University. Science. B》2010,11(9):719-727

Bone morphogenetic proteins (BMPs) play a critical role in the growth and steroidogenesis of granulosa cells (GCs). BMP signals act through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell types. The objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs. A strategy of RNA interference (RNAi)-mediated ‘gene silencing’ of Smad4, a core molecule mediating the intracellular BMP/Smad signal transduction pathways, was used to interrupt endogenous BMP/Smad signaling. Results indicate that Smad4-small interfering RNA (siRNA) caused specific inhibition of Smad4 mRNA and protein expression after transfection. Interrupted endogenous BMP/Smad signaling significantly inhibited growth, and induced apoptosis of porcine GCs, while decreasing estradiol production. In addition, interrupted BMP/Smad signaling significantly (P<0.05) changed the expression of Cyclin D2, CDK4, Bcl-2, and Cyp19a1. These findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.  相似文献   

Metastasis of gastric carcinoma to the thyroid and lung: a case report and review of literature     

Hwa-chan Lee  Fen-fen Chen  Ching-chu Lo  Chung-jing Wang  Wei-chun Lo  Shi-ping Luh 《Journal of Zhejiang University. Science. B》2010,11(7):542-546

Cancer metastasis to the thyroid is extremely rare. The more commonly reported primary sites for metastasis to the thyroid are the kidney, breast, lung, colon, esophagus, and uterus. Thyroid metastasis from the stomach has only been reported in three cases. Herein, we report a 71-year-old man presenting with bilateral thyroid multinodular lesions. Bilateral near-total thyroidectomy was performed due to airway compression with related symptoms. Wedge resection of a suspicious pulmonary nodule, detected on CT, was performed for diagnosis. Polypoid lesions in the stomach were examined by trans-scopic biopsy. Poorly differentiated adenocarcinomas with the same histological profiles were noted at these three sites. The immunohistochemical staining for thyroglobulin of these specimens was negative. We conclude that a new thyroid mass appearing in a patient with present or prior malignancies should raise the concern of metastatic disease.  相似文献   

Arachnoid cell involvement in the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage     

Zhao-liang Xin  Xiao-kang Wu  Jian-rong Xu  Xi Li 《Journal of Zhejiang University. Science. B》2010,11(7):516-523

Objective: To assess if arachnoid cells have the capability to present antigen and activate T-lymphocytes after stimulation by bloody cerebrospinal fluid (CSF), and to illuminate the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage (SAH). Methods: Arachnoid cells were cultured, characterized, and examined by immunofluorescence for the basal expression of human leukocyte antigen-DR (HLA-DR). Expression of HLA-DR, after co-culturing arachnoid cells in vitro with bloody CSF, was investigated by immunofluorescence and flow cytometry (FCM). The variation of arachnoid cells' ultrastructure was observed by transmission electron microscope (TEM). Arachnoid cells were co-cultured with peripheral blood mononuclear cells (PBMCs). The content of soluble interleukin-2 receptor (sIL-2r) in culture medium was detected by enzyme-linked immunosorbent assay (ELISA). Results: (1) Arachnoid cells were successfully cultured for many passages. The immunofluorescent staining was positive for HLA-DR in over 95% of the human arachnoid cells. The punctate HLA-DR was distributed in cytoplasm and not in the karyon. (2) After co-culturing arachnoid cells in vitro with bloody CSF, numerous particles with strong fluorescence appeared in the cytoplasm on Day 6. On Day 8, the quantity of particles and fluorescent intensity were maximal. FCM showed that the percentage of HLA-DR expressing cells was (2.5±0.4)% at the first 5 d, increasing to (60.8±3.6)% on Day 7. (3) After co-culturing arachnoid cells in vitro with bloody CSF, many lysosome and secondary lysosome particles were present in the cytoplasm. Hyperplasia of rough endoplasmic reticulum and enlarged cysts were observed, with numerous phagocytizing vesicles also observed at the edge of the arachnoid cells. (4) Arachnoid cells stimulated by bloody CSF were co-cultured in vitro with PBMCs. The content of sIL-2r in the culture medium, having been maintained at around 1.30 ng/ml during the first 3 d, had increased by Day 4. The content of sIL-2r peaked 7.53 ng/ml on Day 7 and then reduced gradually. Conclusions: (1) Basic HLA-DR expression is present in arachnoid cells. (2) After stimulation by bloody CSF, arachnoid cells have the potential to serve as antigen presenting cells (APCs) and the ability to activate T-lymphocytes, indicating that arachnoid cells are involved in the mechanism of coagulation-initiated inflammation in the subarachnoid space after SAH.  相似文献