pH值敏感水凝胶的合成及药物缓释性能研究   总被引：4，自引：0，他引：4  

钟世华 《湖南城市学院学报》2003,24(3):100-102

聚乙二醇(PEG)与丙烯酰氯反应合成聚乙二醇双丙烯酸酯(PEGDA)，然后以PEGDA、N—乙烯基—2—此咯烷酮(NVP)及丙烯酸为主要原料，N，N^ —亚甲基双丙烯酰胺(BIS)为交联剂，偶氮二异丁腈(AIBN)为引发剂，采用自由基聚合法合成了对pH值变化敏感的N—乙烯基—2—吡咯烷酮—丙烯酸—聚乙二醇双丙烯酸酯交联共聚物(po1y[NVP-AA-PEGDA])水凝胶．利用该共聚物为载体对抗癌药5—氟脲密啶(5—FU)进行包埋，并在SIF及SGF缓冲溶液中于37℃进行体外释药研究．结果表明，在pH值较小的缓释介质中药物释放曲线较平缓，释药周期较长，同时最终的平衡释药百分数也较小；随着样品中PEGDA量的减少，释药曲线趋于平缓，释药周期变长，最终的平衡释药百分数成小．  相似文献   

荧光偏振免疫分析法检测环孢霉素血药浓度     

牛发良  张志华  张秀珑  尹秀玲 《河北北方学院学报(医学版)》2003,20(5):23-26

目的 :建立荧光偏振免疫分析 (FPIA)检测环孢霉素 (Cyclosporine)进行治疗药物监测(TDM)方法 ,为临床器官移植抗排斥反应及多种自身免疫性疾病的治疗服务。方法 :用定标、质控、回收率、精密度、抗干扰等对FPIA进行评价 ,并对用药达稳态浓度后肾移植患者的肝素抗凝全血中的环孢霉素血药浓度进行测定。结果 :定标、质控均符合实验要求 ,平均回收率为96.6% ,平均SD为 5.85 ,CV %为 2 .1 ,高胆红素、血红蛋白、甘油三酯以及高、低总蛋白等对FPIA的干扰误差均 <1 0 %。肾移植患者无药物中毒反应和排斥反应 ,环孢霉素血药浓度平均值为 1 52 .5μg/L。结论 :荧光偏振免疫分析法测定环孢霉素血药浓度具有较高的特异性和稳定性  相似文献   

Substance use among township secondary school students – an emergent phenomenon     

《Africa Education Review》2013,10(1):70-88

Abstract

Despite widespread concern taking the form of condemnation and education about the danger associated with substance use and abuse by students, this threat has not significantly abated. Numerous students still succumb to the allure and destructiveness of inter alia liquor, smoking, sniffing and drugs. This article examines substance use among students by means of a literature study and a questionnaire. The questionnaire obtained the views of the township secondary school students regarding the prevalence of substance use, usual times for substance use, the period of initial and later use, factors influencing students to use substances and the relationships between age and gender and substance use. The study found that the family, peers, community, school and media advertisements exert powerful influence on the students, which has resulted in a considerable number of them using substances. The study concludes with recommendations on how to address the problem.  相似文献   

Visual Chemistry: Three-Dimensional Perception of Chemical Structures     

Balaban  Alexandru T. 《Journal of Science Education and Technology》1999,8(4):251-255

  相似文献   

Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil     

《Electronic Journal of Biotechnology》2021

BackgroundSuper-paramagnetic iron oxide nanoparticles (SPION) contain a chemotherapeutic drug and are regarded as a promising technique for improving targeted delivery into cancer cells.ResultsIn this study, the fabrication of 5-fluorouracil (5-FU) was investigated with loaded Dextran (DEX-SPION) using the co-precipitation technique and conjugated by folate (FA). These nanoparticles (NPs) were employed as carriers and anticancer compounds against liver cancer cells in vitro. Structural, magnetic, morphological characterization, size, and drug loading activities of the obtained FA-DEX-5-FU-SPION NPs were checked using FTIR, VSM, FESEM, TEM, DLS, and zeta potential techniques. The cellular toxicity effect of FA-DEX-5-FU-SPION NPs was evaluated using the MTT test on liver cancer (SNU-423) and healthy cells (LO2). Furthermore, the apoptosis measurement and the expression levels of NF-1, Her-2/neu, c-Raf-1, and Wnt-1 genes were evaluated post-treatment using flow cytometry and RT-PCR, respectively. The obtained NPs were spherical with a suitable dispersity without noticeable aggregation. The size of the NPs, polydispersity, and zeta were 74 ± 13 nm, 0.080 and −45 mV, respectively. The results of the encapsulation efficiency of the nano-compound showed highly colloidal stability and proper drug maintenance. The results indicated that FA-DEX-5-FU-SPION demonstrated a sustained release profile of 5-FU in both phosphate and citrate buffer solutions separately, with higher cytotoxicity against SNU-423 cells than against other cells types. These findings suggest that FA-DEX-SPION NPs exert synergistic effects for targeting intracellular delivery of 5-FU, apoptosis induction, and gene expression stimulation.ConclusionsThe findings proved that FA-DEX-5-FU-SPION presented remarkable antitumor properties; no adverse subsequences were revealed against normal cells.How to cite: Mahdia SA, Kadhimb AA, Albukhaty S, et al. Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.04.001  相似文献   

阿尔茨海默病的动物模型制备及药物治疗     

靳淼  安红梅 《河北北方学院学报(医学版)》2011,(6):28-36

本文综述了近年来阿尔茨海默病(Alzheimer’s disease,AD)的动物模型制备及药物治疗的研究进展.根据AD发病机制的研究,概括了AD动物模型制备的种类,指出目前还缺乏能全面复制病因、病理改变的AD疾病模型.在AD的治疗中,总结了目前的药物治疗种类,提出针对Aβ和Tau蛋白的治疗仍是AD治疗研究热点,中医药在AD治疗中也起到了一定作用,并显示出良好的应用前景.  相似文献   

云南“戒毒康复场所”戒毒模式探索     

房红 《云南师范大学学报(哲学社会科学版)》2011,43(3):151-156

云南省吸毒成瘾严重的吸毒人员比例大,执行强制隔离戒毒的戒毒人员随着时间推移将逐步转入接受社区康复,由于目前社区康复模式建设还不完善,社区康复工作面临巨大压力,严重影响云南省戒毒工作整体成效。我们认为"戒毒康复场所"在实践中获得成效,积累了有益经验,是未来解决云南省社区康复面临的巨大压力的一种有效途径。目前云南省的戒毒康复场所建设速度和规模还远不能满足戒毒需求,建议对"戒毒康复场所"戒毒模式的现有成效推广运用,推动条件成熟的州市积极开办戒毒康复场所,进一步巩固戒毒康复场所的戒毒成效,为戒毒人员搭建回归社会的安全平台。  相似文献   

146例药物不良反应报告分析     

周昌盛 《泰州职业技术学院学报》2010,10(1):22-24

目的了解药物不良反应的发生情况及其给患者造成的危害,分析药物不良反应（ADR）的产生原因,促进临床合理、安全、有效用药。方法对2008年度146份ADR报告进行分类统计和分析。结果以抗感染药引起的不良反应最为常见,涉及的药物共113个品种,占报告的77％,静脉给药是引起药物不良反应的主要给药途径;所累及的器官以皮肤病变最为常见。结论提示临床医生严格掌握药物的适应症,促进合理用药。减少不良反应的发生。  相似文献   

刍议减少中成药制剂不良反应的对策     

顾根网 《泰州职业技术学院学报》2010,10(3):31-33

目的对近年来中成药制剂的不良反应情况报道和分析。方法通过中成药制剂不良反应典型病例的报告。结果说明中成药不良反应,必须引起临床工作的高度重视,研究对策,减少中成药不良反应。结论应该重视中成药制剂新剂型的发展,科学合理用药,对临床应用的重大意义。  相似文献   

合理利用网络视频资源 促进毒品检验课程的教学     

崔连义 《科教文汇》2012,(27):80-81

根据教学目标,合理利用网络视频短片,在解决毒品检验课程教学中的重点与难点,提供多种认知渠道,将抽象的理论直观化,展示实验过程和补充教学资源等方面具有一定的优势。文章从理论的角度阐述了视频短片在毒品检验教学中的作用和优势。从实际教学的角度总结了开发利用视频短片促进毒品检验教学的点滴经验,并以教学实例证明网络视频短片的作用。  相似文献