Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil     

《Electronic Journal of Biotechnology》2021

BackgroundSuper-paramagnetic iron oxide nanoparticles (SPION) contain a chemotherapeutic drug and are regarded as a promising technique for improving targeted delivery into cancer cells.ResultsIn this study, the fabrication of 5-fluorouracil (5-FU) was investigated with loaded Dextran (DEX-SPION) using the co-precipitation technique and conjugated by folate (FA). These nanoparticles (NPs) were employed as carriers and anticancer compounds against liver cancer cells in vitro. Structural, magnetic, morphological characterization, size, and drug loading activities of the obtained FA-DEX-5-FU-SPION NPs were checked using FTIR, VSM, FESEM, TEM, DLS, and zeta potential techniques. The cellular toxicity effect of FA-DEX-5-FU-SPION NPs was evaluated using the MTT test on liver cancer (SNU-423) and healthy cells (LO2). Furthermore, the apoptosis measurement and the expression levels of NF-1, Her-2/neu, c-Raf-1, and Wnt-1 genes were evaluated post-treatment using flow cytometry and RT-PCR, respectively. The obtained NPs were spherical with a suitable dispersity without noticeable aggregation. The size of the NPs, polydispersity, and zeta were 74 ± 13 nm, 0.080 and −45 mV, respectively. The results of the encapsulation efficiency of the nano-compound showed highly colloidal stability and proper drug maintenance. The results indicated that FA-DEX-5-FU-SPION demonstrated a sustained release profile of 5-FU in both phosphate and citrate buffer solutions separately, with higher cytotoxicity against SNU-423 cells than against other cells types. These findings suggest that FA-DEX-SPION NPs exert synergistic effects for targeting intracellular delivery of 5-FU, apoptosis induction, and gene expression stimulation.ConclusionsThe findings proved that FA-DEX-5-FU-SPION presented remarkable antitumor properties; no adverse subsequences were revealed against normal cells.How to cite: Mahdia SA, Kadhimb AA, Albukhaty S, et al. Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.04.001  相似文献   

长期肠内营养患者造瘘管置换术临床应用进展     

李思静  庾艳军  卢杰夫 《大众科技》2015,(1):102-104

对于无法正常吞咽,而具有正常的消化功能的患者,通常医生进行肠内营养,使患者更好的得到营养及药物支持。然而造瘘管并不是永久性的,对于行造瘘术进行肠内营养的患者,多种原因使造瘘管需要进行更换。文章将从造瘘管置换术方法、适应症、并发症及其预防、前景与展望等几方面阐述长期肠内营养患者行造瘘管置换术的现状及最新研究进展。  相似文献   

食管癌根治术胃管意外拔出原因分析及干预对策     

姚明  曾爱民 《科教文汇》2012,(24):143-143

通过对我院2011年1月～12月84例食管癌根治术患者留置的胃管观察,总结胃管意外拔出的原因,包括术前术中术后各阶段的护理风险分析,认为对食管癌根治患者留置胃管采取相应的护理干预,保持其固定牢靠,引流通畅,是减少术后并发症的重要措施,也是手术成功的关键。  相似文献   

乳癌细胞核DNA含量与预后     

雷杰  张振忠  李德仪 《河北北方学院学报(医学版)》1999,(3)

本文应用流式细胞术(Flow Cytometry,FCM)检测了102例石蜡包埋的乳癌组织的细胞核DNA含量,并探讨了DNA指数(DNA Index.DI)和S期细胞比例(S-phase Fraction,SPF)与临床预后因素及病人术后生存状况的关系。结果显示:16例为DNA二倍体,8例近二倍体,78例(77％)非整倍体。非整倍体癌较二倍体/近二倍体癌的SPF高。DI值与组织学分级、淋巴结状况、原发肿瘤大小和临床分期密切相关,而与病人诊断时的年龄无关。淋巴结转移阳性组较阴性组、晚期肿瘤(Ⅲ、Ⅳ期)比早期肿瘤(Ⅰ、Ⅱ期)的DI值高(P<0.05),而非整倍体率仅有降低的趋势但无显著性差异,说明DI值作为一个预后参数比倍性分析统计效率高。SPF与病人的各预后因素及生存情况无明显关系。二倍体/近二倍体癌病人较非整倍体癌病人的生存率高、无瘤生存时间长,说明流式细胞术DNA含量测定确实可为乳癌病人的预后提供有价值的信息。  相似文献   

尿路移行细胞癌雌孕激素受体的免疫组化研究     

李凤歧  马建国  李哲  王寿祥  李向东  苗文隆  薄爱华  戴洁 《河北北方学院学报(医学版)》1999,(1)

为探讨雌激素受体(ER)、孕激素受体(PR)在尿路移行细胞癌中的表达及生物学意义,对64例尿路移行细胞癌石蜡标本进行了免疫组化(SABC法)检测。结果是示:ER、PR阳性率分别为53.1％和50.0％;其中在高分化癌中ER、PR表达高于低分化癌;分级间比较有显著性差异(P<0.05);ER、PR阳性率随肿瘤分期升高而下降,但分期间比较无显著性差异(P>0.05);另外单发肿瘤、多发肿瘤及复发肿瘤之间,男女两性肿瘤之间及尿路各部位之间,ER、PR阳性表达率亦无显著性差异。结论:尿路移行细胞癌ER、PR有较高的表达率.且和分级正相关,提示ER、PR表达可能对尿路移行细胞癌有某种直接或间接的影响,并可作为肿瘤愈后判断指标之一。  相似文献   

麻黄果实总黄酮含量的光学分析     

王书华  邓立宏 《河北北方学院学报(医学版)》2000,(1)

近年来我们对麻黄果实进行了较为全面的研究,结果表明该果实具有抗凝血、降血压等作用。为此我们采用以芦丁为标准,用分光光度法对其总黄酮类物质含量进行了测定。  相似文献   

永川秀芽对SD大鼠的胃损伤预防效果     

付利平  赵欣  易清云  周露  李明霞 《彭城职业大学学报》2014,(1):77-80

对购买的永川秀芽进行SD大鼠胃损伤预防效果评价．通过建立动物模型,研究永川秀芽对胃损伤预防效果．结果表明：高浓度的永川秀芽比低浓度茶降低了血清中IL-6和TNF—α炎症细胞因子水平;永川秀芽处理组大鼠胃黏膜组织的SOD和GSH—Px活力高于对照组,接近正常组;用1000mg／kg永川秀芽灌胃后表现出最强的胃损伤抑制效果．综合分析表明,一定质量分数的永川秀芽具有较好的胃损伤预防效果．  相似文献   

磁共振弥散加权成像对索拉菲尼治疗肝细胞癌的疗效评估(英文)     

Yi-lei ZHAO  ;Qing-qu GUO  ;Gen-ren YANG  ;Qi-dong WANG 《Journal of Zhejiang University. Science. B》2014,15(8):713-719

研究目的:应用磁共振弥散加权成像(DWI)监测荷瘤裸鼠肝细胞性肝癌表观弥散系数(ADC)的变化,探讨ADC对索拉菲尼治疗肝细胞性肝癌的疗效预测。创新要点:探讨磁共振DWI对索拉菲尼治疗肝癌疗效的早期评估,期望能够指导临床合理用药。研究方法:荷瘤裸鼠分成两组(治疗组和对照组)共40只,使用GE 3.0T磁共振成像系统和小动物线圈,分别于服药前后1、10、14和18天(40 mg/kg,一天两次)进行T1加权成像(T1WI)、T2加权成像(T2WI)和DWI扫描。在弥散加权图像上进行ADC测量,对比不同区域和不同时间点的差异。重要结论:索拉菲尼治疗10天后,伴随肝癌肿瘤的缩小,肿瘤内部ADC值明显上升,病理切片显示治疗组瘤体中心小血管闭塞,凋亡细胞可见,此时瘤体内坏死细胞并未大量显现,说明ADC值的改变可以反映索拉菲尼的疗效。  相似文献   

Anticancer Effect of dl-Glyceraldehyde and 2-Deoxyglucose in Ehrlich Ascites Carcinoma Bearing Mice and Their Effect on Liver,Kidney and Haematological Parameters     

R. Kapoor  D. B. Gundpatil  B. L. Somani  T. K. Saha  S. Bandyopadhyay  P. Misra 《Indian journal of clinical biochemistry : IJCB》2014,29(2):213-220

Cancer cells generally exhibit increased glycolysis for ATP generation (the Warburg effect). Compounds that inhibit glycolysis have potential applications in cancer treatment. dl-glyceraldehyde (DLG) and 2-Deoxyglucose (2-DG) have been proven effective in the inhibition of glucose metabolism. Ehrlich ascites carcinoma (EAC) cells were injected intraperitoneally (i.p) in 10–12 weeks old Swiss albino mice, weighing between 20 and 30 g. The anticancer activity of DLG and 2-DG were determined by tumor volume, tumor weight, viable and nonviable tumor cell count, average survival time, percentage increase in life span and tumor inhibition ratio. The blood samples were obtained for biochemical analysis after 9 days of treatment to study the effect on liver, kidney and haematological parameters. Histopathological examination of liver and kidney was also performed. One-way ANOVA test and Dunnett’s test were used for comparisons of parameters in study groups. Both DLG and 2-DG individually decreased the tumor weight, tumor volume, viable tumor cell count and significantly increased the life span of treated mice, however the combination was found to be better. The biochemical parameters of liver and kidney functions and haematological parameters were restored close to control group as compared with the EAC bearing mice. Histopathological examination of liver and kidney in EAC control group showed large areas of necrosis, congestion and mononuclear cell infiltration but such changes were not observed in liver and kidney sections observed after i.p injection of DLG and 2-DG for 9 days. Improvement was much better in the group where combination of these two drugs were used.  相似文献   

HDAC4在乳腺癌中的表达及临床病理学意义     

杨东  崔雪莲  刘双萍  张源 《科教文汇》2014,(31)

目的：研究乳腺癌中HDC4基因蛋白的表达及其临床病理学意义。方法：实验共选取91例乳腺组织的存档蜡块标本,其中包括46例乳腺癌、26例导管内原位癌（DCIS）以及19例正常宫颈上皮组织。应用免疫组织化学（SP法）检测HDC4蛋白在上述组织中的表达,并分析其蛋白检测的临床病理学意义。结果：与正常乳腺组织（15.8%,3/19）相比,HDC4蛋白在DCIS和乳腺癌组织中表达率明显增高,分别达46.2%(12/26)和71.7%(33/46),强阳性表达率在DCIS和乳腺癌组织中分别达19.2%（5/26）和45.6%（21/46）,也明显高于正常乳腺组织,差异显著（p<0.01）。结论：HDC4蛋白表达可作为女性乳腺癌及原位癌DCIS的病理辅助诊断指标之一,可能有助于乳腺癌的诊断及预后评估。  相似文献