Transcriptomic Analysis of Aflatoxin B1-Regulated Genes in Rat Hepatic Epithelial Cells     

杨柳  季静  李光辉  李君文  陈招立  王海勇 《天津大学学报(英文版)》2014,(6):451-457

Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety of liver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profiles of aflatoxin B1-induced rat hepatic epithelial cells. The expression of 325, 184 and 199 special genes was altered when exposed to 0.03, 0.1 and 0.2 μmol/L aflatoxin B1 respectively, and 239 genes were commonly expressed. After the functional analysis on these dose-special genes, we determined several key pathways related to hepatotoxicity, such as TGF-beta signaling pathway, tight junction, adherens junction, the regulation of actin cytoskeleton, Erb B signaling pathway, p53 signaling pathway, pathways in cancer and axon guidance. Common genes were mainly associated with focal adhesion, ECM-receptor interaction, and cell adhesion molecules. Gene ontology annotations showed a good concordance with these pathways. The quantitative real-time polymerase chain reaction(PCR) analysis of selected genes showed similar patterns in microarrays. The toxicogenomic study provides a better understanding of molecular mechanisms of aflatoxins.  相似文献   

徽州文化的基因、特质与解构     

章尚正 《合肥教育学院学报》2014,(5):70-77

徽州文化是代表中国明清社会从“农业的系统”向“商业的系统”转变之际的特异性与先导性的地域文化标本。徽州文化的独特性取决于它的文化基因,包括移民型人口结构、宗法制社会结构、商贾为重的经济结构与崇儒尊孔奉朱的精神结构。徽州文化的特质是既脱胎于中华文化母体,又成为新兴商业文化的先驱者,成为新旧文化二律背后的共存体,表现出许多彼此矛盾又自我调谐的文化表征,其中最典型的为：社会价值与人生价值观上的崇儒与重商,人生态度上的内敛与进取,生活方式上的节俭与时尚。徽州文化解构、降格为普通地域文化,最根本、最关键的动因是构成徽文化特质的内在基因产生了变异：移民型人口结构发生反向移民,宗法制社会结构彻底瓦解,以商贾为重的经济结构被迫转型,崇儒尊孔奉朱的精神结构逐渐涣散,而这种种基因的嬗变受制于内外环境的变革压力。  相似文献   

Deregulation of microRNA expression in thyroid tumors简     

Zi-ming YUAN  Zhi-li YANG  Qi ZHENG 《Journal of Zhejiang University. Science. B》2014,15(3):212-224

MicroRNAs (miRNAs or miRs) are endogenous non-coding RNAs that negatively regulate gene expression by binding to the 3′ non-coding regions of target mRNAs, resulting in their cleavage or blocking their translation. miRNAs may have an impact on cell differentiation, proliferation, and survival, and their deregulation can be inclined to diseases and cancers, including thyroid tumors. The purpose of this review is to summarize the existing findings of deregulated miRNAs in different types of thyroid tumors and to exhibit their potential target genes, especially to demonstrate those involved in tumor invasion and metastasis. In addition, new findings of circulating miRNA expression profiles, single nucleotide polymorphism (SNP) in thyroid tumors, and the correlation of somatic mutations with deregulated miRNA expression in thyroid tumors were all included in this review.  相似文献   

植物基因工程的应用与展望   总被引：1，自引：0，他引：1  

李红梅  王林  陈娟 《玉溪师范学院学报》2006,22(12):87-90

介绍了植物基因工程的研究内容及植物基因工程技术在病虫害控制、杂草控制、改良农作物品质、农作物育种、花卉品质的改良等方面的运用现状及前景.  相似文献   

肥胖及相关问题的认识(综述)   总被引：3，自引：0，他引：3  

刘刚  黄玉山 《山东体育学院学报》2001,17(3):28-30

有关肥胖机制的研究很多,提出了各种假说.对相关的肥胖危害问题也进行了研究,提出了不同的观点,就其相关知识进行总结.  相似文献   

高黎贡山植物资源开发   总被引：1，自引：0，他引：1  

刘经伦 《保山师专学报》2003,22(5):6-10

植物界作为数十亿年的进化产物，其庞大的种群及数量潜藏着许多未知和可能。如基因资源、人工无法合成的特殊天然物质等，正影响、改变着我们的生活。高黎贡山以其独特的地理、自然条件，使其孕育着丰富的、多样的、独特的植物资源，开发潜力巨大。  相似文献   

广西20～39岁成年人体质因子结构的研究     

李健  姚辉洲 《北京体育大学学报》2007,30(2):218-220

运用文献研究法、调查法和数理统计法,对影响广西20～39岁成年人体质的主要因子及其因子结构特点进行研究.研究表明:影响其体质的最重要的因子为身体围度因子和体育锻炼因子,较重要的因子是呼吸因子和主要身体素质因子.各因子的重要程度具有性别特点.呼吸因子由身高与肺活量构成.  相似文献   

对Cluberton等“糖类的补充对运动引起的代谢基因表达改变的影响”一文的述评          下载免费PDF全文

王香生  陈亚军 《体育科研》2012,33(4):8-10

本文所推荐的论文是一篇较有代表性的、值得学习和借鉴的优秀科研报道。研究背景文献清楚;研究命题创新性强,具较高的理论和实践意义;试验设计简单合理;实验过程控制严谨;指标测试体系有代表性;讨论部分逻辑清晰,层层推进;结论简洁,合理。当然,本研究当中也存在一些不足之处。例如：虽然此研究中采用了自身对照的实验方法,但仍无法回避样本量太小的事实。另外,营养学研究中身体基线状态的控制也应值得注意,无论是受试者运动前几天的饮食状况,还是训练情况都需要进行严格控制,这对于保持受试者每次测试前的生理状态和营养状态的一致性是很有必要的。  相似文献   

力竭运动后不同时相大鼠心肌Egr-1蛋白的变化及其在运动性心肌微损伤发生中的作用          下载免费PDF全文

袁旭鑫  常芸 《体育科研》2012,33(4):30-34

目的：探讨力竭运动后不同时相心肌早期生长应答基因Egr-1在蛋白水平的变化特点,为运动性心肌微损伤及心肌功能异常发生机制的阐释提供实验依据。方法：100只健康成年雄性SD大鼠,分为一次力竭游泳运动组、两周反复力竭游泳运动组及安静对照组,分别于力竭运动后即刻、6 h、12 h及24 h取材,应用免疫荧光组化技术和图像分析方法研究大鼠心肌Egr-1蛋白含量的变化。结果：一次力竭运动组大鼠心脏各部位Egr-1蛋白含量即刻组与对照组相比显著性升高（P〈0.05）,24 h组与对照组相比显著性降低（P〈0.05）;反复力竭运动组大鼠心脏各部位Egr-1蛋白含量即刻组与对照组相比显著性升高（P〈0.05）,6 h组、12 h组、24 h组大鼠心脏各部位Egr-1蛋白含量均显著低于对照组（P〈0.05）。结论：不同力竭运动后心脏各部位Egr-1在运动后即刻明显升高,表明心肌细胞Egr-1对力竭运动刺激产生快速应激反应,可能诱导炎性细胞的趋化、聚集以及氧自由基的产生。而心脏各部位Egr-1在力竭运动后24 h内恢复到正常水平,说明力竭运动后心脏Egr-1改变属早期应激反应,且此反应属可复性改变。  相似文献   

Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination     

Li QY  Niu HB  Yin J  Shao HB  Niu JS  Ren JP  Li YC  Wang X 《Journal of Zhejiang University. Science. B》2010,11(11):862-870

A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl₃ on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl₃ treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl₃ during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.  相似文献