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目的：探讨耐药基因p-糖蛋白（p-gp）、谷胱甘肽-S-转移酶（GST-Ⅱ）、拓扑异构酶Ⅱ（TOpoⅡ）、胸苷酸合成酶（TS）在宫颈癌中的表达及临床意义。方法：应用免疫组织化学方法检测65例宫颈癌中p-gp、TOpoⅡ、GST-π、TS的表达，并分析其与临床预后之间的关系。结果：p-gp的表达与肿瘤分化程度、淋巴结转移有关（P〈0．05），与UICC分期、局部复发无关（P〉0．05）；TOpoⅡ的表达与淋巴结转移、局部复发有关（P〈0．05），与肿瘤分化程度、UICC分期无关（P〉0．05）；GST-π的表达与各个指标之间均存在相关（P〈0．05）；TS与淋巴结转移、UICC分期有关（P〈0．05），与局部复发、肿瘤分化无关（P〉0．05）。经多因素COX比例风险模型分析显示：局部复发、淋巴结转移及TOPOⅡ、GST-π的表达与宫颈癌患者术后生存率有关（P〈0．05）。结论：宫颈癌中p-gp、TOpoⅡ、GST-π、TS的联合检测对判断宫颈癌的预后十分必要；局部复发、淋巴结转移及TOpoⅡ、GST-π的表达可作为宫颈癌患者独立的预后因素。 相似文献
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ＣａｓｅＲｅｐｏｒｔ:Ａ 4 7 ｙｅａｒ ｏｌｄｍａｎｈａｄｗｉｔｈａｎｉｎｔｅｒｍｉｔｔｅｎｔｇｒｏｓｓｈｅｍａｔｕｒｉａｆｏｒｍｏｒｅｔｈａｎｏｎｅｙｅａｒ.Ｏｎｐｈｙｓｉｃａｌｅｘａｍｉｎａｔｉｏｎｔｈｅｋｉｄｎｅｙｓｗｅｒｅｎｏｔｐａｌｐａｂｌｅａｎｄｔｈｅｒｅｗｅｒｅｎｏｂｉｌａｔｅｒａｌｃｏｓｔｏｖｅｒ ｔｅｂｒａｌａｎｇｌｅｔｅｎｄｅｒｎｅｓｓ.Ｌａｂｏｒａｔｏｒｙｉｎｖｅｓｔｉｇａｔｉｏｎｒｅｓｕｌｔｓｗｅｒｅｎｏｒｍａｌｅｘｃｅｐｔｆｏｒｈｅｍａｔｕｒｉａ .Ｓｕｓ ｐｅｃｔｅｄｃａｎｃ… 相似文献
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Objective: To investigate the in-vitro antitumor immune responses of dendritoma formed by mouse hepatocellular carcinoma (HCC) cells and lymphotactin (Lptn) gene modified dendritic cells (DCs). Method: DCs prepared from mouse bone marrow were genetically modified by lymphotactin adenovirus, and fused with H22 cells by polyethylene glycol (PEG). RT-PCR and ELISA were employed to identify lymphotactin expression at mRNA and protein level. Cell phenotypes and fusion efficiency was detected by FACS. The stimulatory effect of DC on T cells was detected by mixed lymphocyte reaction. The cytotoxicity activity against H22 cells was assayed by LDH method. Results: Lymphotactin could be efficiently expressed by DCLptn/H22 hybridoma. DCLptn/H22 cells could induce potent T cell proliferation effect and generate strong cytotoxic T lymphocyte (CTL) reaction against allogenic H22 cells. Conclusion: Lymphotactin genetic modification could enhance the in vitro immune activity of the dendritoma. 相似文献
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采用S-P免疫组织化学的方法,配对检测了37例大肠癌组织及同一病人的正常粘膜上皮中p53蛋白的表达情况。结果表明,p53蛋白表达阳性19例,阴性18例,阳性率为51.4％,而配对的大肠正常粘膜检测结果均呈p53阴性;p53蛋白表达与病人性别、年龄、Dukes分期等因素无明显相关性,而与淋巴结转移、浸润深度和不同分化程度等存在明显相关性。提示p53蛋白的检测可以作为大肠癌病人病情诊断和预后分析的辅助手段。 相似文献
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Liu Y Chen L Peng SY Chen ZX Hoang-Vu C 《Journal of Zhejiang University. Science. B》2005,6(9):913-918
Objectives: To explore the mechanism of development and aggressiveness in gastric carcinomas by investigating the expression and role of CD97 and its cellular ligand CD55 in gastric carcinomas. Methods: Tumor and corresponding normal mucosal tissue, collected from 39 gastric carcinoma patients, were examined by immunohistochemistry and RT-PCR for the expression of CD97 and CD55. Results: CD97stalk was strongly stained on scattered tumor cells or small tumor cell clusters at the invasion front of gastric carcinomas. The expression of CD97stalk was frequently observed in tumors of stage Ⅰ and T1 gastric carcinoma patients. The expression of CD97stalk between Stage Ⅰ and Stage Ⅱ, Ⅲ, Ⅳ specimens showed significant difference (P＜0.05), between T1 and T2, T3, T4 specimens also showed significant difference (P＜0.05). Specimens with tumor invasion depth limited in mucosa of T 1 specimens showed higher positive CD55 expression than specimens with the same tumor invasion depth in T2, T3, T4 specimens, the expression of CD55 between T1 and T2, T3, T4 specimens was significantly different (P＜0.05).There was strong correlation between the distribution patterns of CD97stalk and CD55 on tumor tissues (r=0.73, P＜0.05). Signet ring cell carcinomas frequently contained strong CD97stalk and CD55-staining. Conclusions: Our results suggest that CD97stalk is probably involved in the growth, invasion and aggressiveness of gastric carcinomas by binding its cellular ligand CD55. CD97stalk and CD55 could be useful as molecular markers for prognosis and therapy of gastric carcinoma patients. 相似文献
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Objective: To evaluate the expression of matrix metalloproteinase-9 (MMP9) in nasopharyngeal carcinoma and the association between MMP9 and Epstein-Barr virus infection. Methods: The MMP9 expression was studied by immunohistochemical analysis; and Epstein-Barr virus encoded small nuclear mRNA-1 (EBER-1) produced by in situ hybridization were examined in 41 nasopharyngeal carcinoma sections, and the relation between them, and the associations of MMP9 with clinical features were statistically analyzed. Results: Positive expression rate of MMP9 was 73.17%. The expression of MMP9 showed significant positive correlation with the expression of EBER- 1 (γ=0.483, P=0.001). There was significant association of MMP9 expression with lymph nodes metastasis and clinical stage (P＜0.001), non-significant association with age, gender, pathological classification and T classification. Conclusions: The highly pronounced expression of MMP9 is associated with cervical lymph nodes metastasis. Epstein-Barr virus can enhance NPC metastasis by up-regulating the expression of MMP9. 相似文献
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目的:研究热休克蛋白27(Heat Shock Protein27)在乳腺癌中的表达.方法:采用免疫组化方法检测48例乳腺癌组织中的HSP27.结果:HSP27在乳腺癌中阳性表达率为54.17%(26/48).在非浸润性癌和浸润性癌中阳性表达率为75.00%(3/4)和52.27%(23/44).HSP27阳性表达在非浸润性癌和浸润性癌中无明显差别(P＞0.05).HSP27阳性表达与有无淋巴结转移无关(P＞0.05).结论:在乳腺癌中HSP27有较高表达,检测乳腺癌中HSP27对化疗有指导作用. 相似文献
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目的为探讨P_(53)、PCNA抗原在胃粘膜中的表达情况及其与胃癌发生的关系方法：应用LsAB免疫织化方法对80例胃粘膜进行了检测结果显了胃癌中P_(53)、PCNA强阳性表达率（80％、93．3％）明显高于异型增生（467％,53．3％）异型增生中P_(53)、PCNA强阳性表达率明显高于慢性萎缩性胃炎及慢性浅表性胃炎．结论研究结果表明,检测P_(53)、PCNA在肖粘膜中的不同表达,对胃癌发生机理的认识有重要意义． 相似文献
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环氧化酶-2基因在乳腺癌组织中的表达 总被引：2，自引：0，他引：2
目的:探讨环氧化酶-2(cos-2)在乳腺癌中的表达特点及其与临床病理参数的相关性.方法:采用免疫组织化学SP法,检测60例乳腺癌组织中环氧化酶-2的表达情况.结果:60例乳腺癌组织中环氧化酶-2阳性表达率为78.33%.环氧化酶-2在临床分期Ⅰ～Ⅱ期的阳性率(83.67%)明显高于Ⅲ～Ⅳ期阳性率(54.55%),两组间的表达有显著差异(P<0.05).在有、无淋巴结转移组间,环氧化酶-2阳性表达也有显著差异(P<0.05);而在不同年龄组、病理类型及左右乳分组中的阳性表达差异无显著性.结论:环氧化酶-2在肿瘤形成的早期过程中产生,可能参与或促进肿瘤的形成.环氧化酶-2的检测可能对乳腺癌的早期诊断有实际意义. 相似文献
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目的:探讨ER、erbB-2和P53在胃癌表达及相关性。方法:取胃癌手术标本119例,SP免疫组织化学染色。结果:ER、erbB-2和P53在胃癌表达率分别为63.87％,75.51％和73.11％;三者同时阳性者31.93％;ER与P53同时阳性者37.82％;ER与erbB-2同时阳性者33.61％;ER在高分化组阳性率高于低分化组(P<0.05);而erbB-2和P53在低分化组阳性率高于高分化组。70岁以上组P53、ER和erbB-2表达率均较高。结论:ER、P53和erbB-2在胃癌中表达率与癌瘤分化程度相关。P53和erbB-2有相关性,而且它们是胃癌预后不良的指标。 相似文献