Oxidative stress and antioxidants in tubercular meningitis     

K Sudha  Ashalatha V Rao  S. N. Rao  Anjali Rao 《Indian journal of clinical biochemistry : IJCB》2002,17(1):34-41

Oxidative stress is implicated in the etiopathogenesis of a variety of human diseases. Therefore, in the present study, erythrocyte lipid peroxidation, percentage hemolysis, antioxidant enzymes viz., glutathione reductase, glutathione peroxidase, superoxide dismutase and plasma antioxidants viz., ceruloplasmin, vitamins A,E and C have been determined in 19 patients with tubercular meningitis (TBM) and 50 normals. Six patients who were treated with antibiotics were considered for the follow up. The statistical analysis was carried out by Mann Whitney U test and Wilcoxon rank sum test. Lipid peroxidation (P<0.02), percentage hemolysis (P<0.001) and plasma ceruloplasmin (P<0.0001) of TBM patients were significantly higher, whereas erythrocyte glutathione reductase (P<0.05) and plasma antioxidant vitamins A, E and C (P<0.01, P<0.05 respectively) were significantly lower than those of the controls. In the follow up patients the glutathione reductase and catalase levels were significantly high (P<0.05) compared to their pre-treated condition. Vitamin C and E levels have attained normal range. This study indicated that the blood antioxidant status of TBM patients which was low compared to controls improved after treatment, suggesting the role of free radicals in TBM.  相似文献   

早期饲喂对肉鸡生长性能和肠道消化酶的影响     

魏刚才  郑爱武  谢红兵  张金州  常新耀  刘保国  王永强 《河南职业技术师范学院学报》2014,(1):38-42

为研究早期饲喂对肉鸡生长性能和肠道消化酶的影响,选择360只刚出壳的AA肉用雏鸡,采用单因子试验设计,随机分成4个处理组,每组4个重复,每个重复20只,试验1组出壳后12 h饲喂,试验Ⅱ组出壳24 h后饲喂,试验Ⅲ组出壳36 h后饲喂,对照组出壳48 h后饲喂.饲喂相同日粮,试验期为6周.于21日龄和42日龄分别测定肉鸡进行生产性能和酶活性.结果表明：早期饲喂显著提高了肉鸡的日增重,降低了料重比,试验Ⅰ组和试验Ⅱ组与对照组相比,日增重分别提高了4.19%和3.70%,料重比分别降低5.61%和5.10%,差异显著（P〈0.05）;试验Ⅰ组、试验Ⅱ组和试验Ⅲ组肠道消化酶水平高于对照组.早期饲喂提高了肉鸡肠道消化酶水平,以出壳后12 h饲喂最好.  相似文献   

脑卒中后无感知型尿失禁患者的临床特点与预后     

沈骏  周仁华 《泰州职业技术学院学报》2014,(6):68-70

目的比较紧迫型尿失禁（Urge urinary incontinence,UUI）与无感知型尿失禁（urinary incontinence with impaired awareness,IA-UI）的临床特点与其对预后的影响。方法连续收集258例住院脑卒中患者,其中有58例有尿失禁（UUI20例,IA-UI38例）。收集其临床一般资料和脑影像学特征。结果 （1）基线时,与UUI患者比较,IA-UI患者IQCODE、BI、MMSE和SINIS评分,前循环卒中发生率,顶叶新病灶有显著统计学差异（P〈0.05）。（2）随访一年后,38例IA-UI患者中有11例死亡,仅有2例恢复自理能力;20例UUI患者中仅仅1例死亡,12例恢复自理能力,差异显著具有统计学意义（P〈0.05）。结论脑卒中后无感知型尿失禁的临床特征和对预后影响方面与紧迫型尿失禁患者不同,无感知型尿失禁可能提示更严重的脑损伤。这说明卒中相关性尿失禁有多种尿动力学模式。  相似文献   

Diagnostic accuracy of UriSed automated urine microscopic sediment analyzer and dipstick parameters in predicting urine culture test results     

Ka?an Huysal  Yasemin U. Budak  Ayse Ulusoy Karaca  Murat Aydos  Serdar Kahvecio?lu  Mehtap Bulut  Murat Polat 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2013,23(2):211-217

Introduction

Urinary tract infection (UTI) is one of the most common types of infection. Currently, diagnosis is primarily based on microbiologic culture, which is time- and labor-consuming. The aim of this study was to assess the diagnostic accuracy of urinalysis results from UriSed (77 Electronica, Budapest, Hungary), an automated microscopic image-based sediment analyzer, in predicting positive urine cultures.

Materials and methods:

We examined a total of 384 urine specimens from hospitalized patients and outpatients attending our hospital on the same day for urinalysis, dipstick tests and semi-quantitative urine culture. The urinalysis results were compared with those of conventional semi-quantitative urine culture.

Results:

Of 384 urinary specimens, 68 were positive for bacteriuria by culture, and were thus considered true positives. Comparison of these results with those obtained from the UriSed analyzer indicated that the analyzer had a specificity of 91.1%, a sensitivity of 47.0%, a positive predictive value (PPV) of 53.3% (95% confidence interval (CI) = 40.8–65.3), and a negative predictive value (NPV) of 88.8% (95% Cl = 85.0–91.8%). The accuracy was 83.3% when the urine leukocyte parameter was used, 76.8% when bacteriuria analysis of urinary sediment was used, and 85.1% when the bacteriuria and leukocyturia parameters were combined. The presence of nitrite was the best indicator of culture positivity (99.3% specificity) but had a negative likelihood ratio of 0.7, indicating that it was not a reliable clinical test.

Conclusions:

Although the specificity of the UriSed analyzer was within acceptable limits, the sensitivity value was low. Thus, UriSed urinalysis results do not accurately predict the outcome of culture.  相似文献   

Pre-exercise antioxidant enzyme activities determine the antioxidant enzyme erythrocyte response to exercise     

P Tauler  A Aguiló  P Guix  F Jiménez  G Villa  JA Tur 《Journal of sports sciences》2013,31(1):5-13

Free radical production increases during exercise and oxidative damage occurs in several tissues. We examined the effects of three different exercise tests on the pattern of change of erythrocyte enzyme antioxidant activities. The tests were a short maximal exercise test, a submaximal prolonged exercise test and a cycling stage during competition. The participants were amateur and professional cyclists with different training statuses and different basal erythrocyte antioxidant enzyme activities. The maximal test produced no changes in the erythrocyte antioxidant enzyme activities of amateur sportsmen. The submaximal test, performed at 80% of maximal oxygen uptake, decreased erythrocyte catalase (12%), glutathione peroxidase determined with H₂O₂ (14%) and glutathione reductase (16%); superoxide dismutase activity increased by about 25%. The cycling stage performed by professional cyclists increased erythrocyte catalase (29%) and glutathione reductase (10%) activities. The in vivo changes in glutathione reductase activity were confirmed by in vitro measurements: hydrogen peroxide decreased and the presence of catalase increased the activity of this enzyme. In conclusion, we suggest that the different erythrocyte antioxidant enzyme responses to diverse exercise tests can be explained by the effects of hydrogen peroxide and the superoxide anion on the antioxidant enzyme activities in erythrocytes.  相似文献   

麻醉前后插留置导尿管的比较研究     

沈莉 《湖州师范学院学报》2002,24(3):81-82,87

对观察麻醉前后行留置导尿术对病人的不同影响，随机抽取200例腹部手术病人，分成二组，分别于麻醉前及麻醉后5分钟行留置导尿术，发现麻醉后行留置导尿术可明显减轻病人疼痛、排尿困难等症状，取得了较好的临床效果。  相似文献   

尿纤维连接蛋白在诊断膀胱移行细胞癌中的应用价值     

凌海滨  赵莹  李向东  苗文隆  李凤歧  成日青 《河北北方学院学报(医学版)》2007,24(5):8-12

目的:评价尿纤维连接蛋白(fibronectin,Fn)在诊断膀胱移行细胞癌中的应用价值,并界定其临床参考值。方法:采用ELISA法检测55例膀胱移行细胞癌患者、34例其他泌尿系疾病患者及29例正常人的尿Fn含量,并同时测定尿肌酐(Creatin,Cr),计算Fn/Cr比值,比较各组人群尿Fn和尿Fn/Cr比值之间的差异;分析不同肿瘤分期、分级尿Fn和尿Fn/Cr之间的差异;利用尿Fn/Cr比值与其它尿生物标志物比较它们之间的优势。结果:膀胱癌组尿Fn含量(583.4±302.5μg/L)、Fn/Cr比值(149.5±93.3mg/mol)与其他泌尿系疾病组Fn含量(209.3±125.0μg/L)、Fn/Cr比值(52.4±36.2mg/mol)以及对照组Fn含量(134.3±83.6μg/L)、Fn/Cr比值(38.2±22.7mg/mol)相比,有显著性差异(P<0.01,P<0.05)。尿Fn诊断膀胱移行细胞癌的灵敏度和特异度分别为78.2%和76.2%,尿Fn/Cr比值分别为81.8%和79.4%。另外,不同分期、分级膀胱癌患者的尿Fn含量及Fn/Cr比值也有明显差异(P<0.05)。比较后提示尿Fn/Cr比值与目前多种分子生物学指标检测膀胱肿瘤的敏感性、特异性基本相似。结论:尿Fn含量及尿Fn/Cr比值在膀胱癌的临床诊断方面有重要的价值,是诊断膀胱癌的一种较理想指标;尿Fn含量及Fn/Cr的高低与膀胱癌的分期、分级有关,可大致判断膀胱癌的浸润度和恶性度,并可用于膀胱癌的预后评估,但目前仍不能代替膀胱镜;界定其临床参考值为250μg/L(Fn)和62mg/mol(Fn/Cr)。  相似文献   

Probing transient protein-mediated DNA linkages using nanoconfinement     

Maedeh Roushan  Parminder Kaur  Alena Karpusenko  Preston J. Countryman  Carlos P. Ortiz  Shuang Fang Lim  Hong Wang  Robert Riehn 《Biomicrofluidics》2014,8(3)

We present an analytic technique for probing protein-catalyzed transient DNA loops that is based on nanofluidic channels. In these nanochannels, DNA is forced in a linear configuration that makes loops appear as folds whose size can easily be quantified. Using this technique, we study the interaction between T4 DNA ligase and DNA. We find that T4 DNA ligase binding changes the physical characteristics of the DNA polymer, in particular persistence length and effective width. We find that the rate of DNA fold unrolling is significantly reduced when T4 DNA ligase and ATP are applied to bare DNA. Together with evidence of T4 DNA ligase bridging two different segments of DNA based on AFM imaging, we thus conclude that ligase can transiently stabilize folded DNA configurations by coordinating genetically distant DNA stretches.  相似文献   

艾灸预处理对健美操运动后尿亚硝酸盐的影响     

崔荣荣  赖亮亮 《辽宁体育科技》2010,32(6):31-32

目的：通过对温州医学院在校大学生进行艾灸预处理,观察艾灸预处理前后健美操运动后尿亚硝酸盐的变化情况,从而探讨艾灸预处理对于运动后尿亚硝酸盐的影响和机制。方法：对8名普通在校大学生双侧足三里穴进行为期7天的艾灸,并分别于艾灸前后测定健美操运动后尿亚硝酸盐的变化。结果：艾灸预处理后尿亚硝酸盐全部由阳性转变为阴性。结论：艾灸预处理能降低运动后尿亚硝酸盐的含量。  相似文献   

间歇运动训练和急性间歇运动对缺血/再灌注大鼠心电图及血清心肌酶的影响     

彭峰林  郭艳菊  张林 《中国体育科技》2010,46(3)

目的:探索长期运动训练和急性运动的心脏保护作用及心电指标的变化规律,为进一步研究运动预适应机制提供依据.方法:32只3月龄雌性SD大鼠随机分成3大组:间训模型组(n=8)、急性运动模型组和对照组(n=8),间歇运动训练组进行高强度间歇运动训练,急性间歇运动组仅进行急性高强度的间歇运动,对照组(n=16)不运动,在心脏模型制备时对照组随机分成对照模型组(n=8)和对照假手术组(n=8).运动训练结束后,各组大鼠均采用结扎左冠状动脉制备在体大鼠心肌缺血/再灌注损伤模型,但对照假手术组只开胸穿线而不结扎.缺血/再灌注过程实行全程心电监控,缺血30 min、再灌注40 min后,测定血清心肌酶.结果:经间歇运动训练和急性间歇运动处理的缺血/再灌注大鼠血清心肌酶明显低于对照模型组(P<0.05或P<0.01);缺血30 min及再灌注40 min间歇运动训练组心电图ST段、T波面积和QT间期明显低于对照模型组(P<0.05或P<0.01);再灌注40min急性间歇运动组心电图ST段、T波和QT间期明显低于对照模型组(P<0.05).结论:长期间歇运动训练产生心肌抗缺血/再灌注损伤的保护作用,急性运动主要产生抗心脏再灌注损伤的保护作用.  相似文献