Isolation and characterization of mitochondria from goat hearts     

Tester F. Ashavaid Ph.D.  FACB  Neena S. Kumbhat 《Indian journal of clinical biochemistry : IJCB》2005,20(1):72-79

Cardiac mitochondria provide energy for the contraction/relaxation cycle. The aim of our study was to isolate and characterize mitochondria from Caprine hearts under control and in-vitro induced ischemia. A decrease in activities of all the enzymes was observed in the ischemic models. Further characterization of proteins was done by SDS-PAGE and BN-PAGE. Lipids have been characterized by analyzing the phospholipids by HPTLC and fatty acids by GLC in both groups. Our results indicated that injury occurs early in the course of ischemia and progresses during ischemia. TBARS and carbonyl content have also been measured. The in-vitro effects of fatty acids have been studied on the enzymes and complexes of mitochondria.  相似文献   

Hepatoprotective effect of<Emphasis Type="Italic">Hygrophila spinosa</Emphasis> and<Emphasis Type="Italic">Cassia occidentalis</Emphasis> on carbon tetrachloride induced liver damage in experimental rats     

K. Usha  G. Mary Kasturi  P. Hemalatha 《Indian journal of clinical biochemistry : IJCB》2007,22(2):132-135

The present study was undertaken to analyze the levels of some known antioxidant (both enzymic and non enzymic) activities in the rootsof Hygrophila spinosa andCassia occidentalis also to find out the hepatoprotective effect of the same in carbon tetrachloride induced liver damage in albino rats. The roots were found to be rich in antioxidants. Liver damage in rats were induced by carbon tetrachloride. To find out the hepatoprotective activity, the aqueous extract of the plant root samples were administrated to rats for 15 days. The serum marker enzymes Aspartate transaminase, Alanine transaminase and Gama Glutamyl were measured in experimental animals. The increased enzyme levels after liver damage with carbon tetrachloride were nearing to normal value when treated with aqueous extract of the root samples. Histopathological observation also proved the hepatoprotectivity of the root samples.  相似文献   

冷激诱导耐冷性过程中小麦幼苗抗氧化酶的变化     

郭丽红  王定康  王德斌  陈雪  程威  陈善娜 《昆明师范高等专科学校学报》2007,29(4):71-74

采用小麦幼苗为材料，研究了冷激诱导抗冷性过程中超氧化物歧化酶（SOD），过氧化氢酶（CAT），抗坏血酸专一性过氧化物酶（APX）、愈创木酚过氧化物酶（GPX）四种抗氧化酶活性的变化，结果发现冷激可以提高SOD，CAT，APX，GPX四种抗氧化酶的活性，表明冷激诱导的抗冷性与SOD，CAT，APX，GPX四种抗氧化酶活性增加有关，暗示SOD，CAT，APX，GPX四种抗氧化酶在冷激诱导小麦幼苗抗冷性的形成过程中起重要作用。  相似文献   

Protective Effect of Emblica officinalis Against Alcohol-Induced Hepatic Injury by Ameliorating Oxidative Stress in Rats     

Damodara Reddy V  Padmavathi P  Gopi S  Paramahamsa M  Varadacharyulu NCh 《Indian journal of clinical biochemistry : IJCB》2010,25(4):419-424

The effect of Emblica officinalis fruit extract (EFE) against alcohol-induced hepatic damage in rats was investigated in the present study. In vitro studies showed that EFE possesses antioxidant as well nitric oxide (NO) scavenging activity. In vivo administration of alcohol (5 g/kg b.wt/day) for 60 days resulted increased liver lipid peroxidation, protein carbonyls, nitrite plus nitrate levels. Alcohol administration also significantly lowers the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and reduced glutathione as compared with control rats. Administration of EFE (250 mg/kg body weight) to alcoholic rats significantly brought the plasma enzymes towards near normal level and also significantly reduced the levels of lipid peroxidation, protein carbonyls and restored the enzymic and non-enzymatic antioxidants level. This observation was supplemented by histopathological examination in liver. Our data indicate that the tannoid, flavonoid and NO scavenging compounds present in EFE may offer protection against free radical mediated oxidative stress in rat hepatocytes of animals with alcohol-induced liver injury.  相似文献   

纳米酶：结合天然酶和人工催化的力量          下载免费PDF全文

杜鹏  高利增  焦健  范克龙  阎锡蕴 《中国科学院院刊》2024,39(5):809-820

纳米酶代表了一类新型人工酶和生物催化剂,打破了无机与有机生命的界限。它既有纳米材料的理化特性,也有独特的类酶催化活性。同时,这些理化特性有可能会调控催化活性,使得纳米酶跟天然酶、传统的模拟酶和化学催化剂区别开来。纳米酶有比较好的稳定性、耐高温、低温、耐酸碱、活性可调且多功能,目前受到了广泛关注,在生物医药、环境治理、绿色农业、新能源等领域展现出巨大的应用前景,并初步形成了相应的学科框架。为了更好地推动纳米酶的发展,拓展对纳米酶的认识,文章回顾了纳米酶发现,分析凝练了纳米酶的学科特点及其结构,综述了纳米酶的应用,并展望了未来的发展趋势。  相似文献   

Metagenomics approach to identify lignocellulose-degrading enzymes in the gut microbiota of the Chinese bamboo rat cecum     

《Electronic Journal of Biotechnology》2021

BackgroundLignocellulose is considered a renewable organic material, but the industrial production of biofuel from lignocellulose is challenging because of the lack of highly active hydrolytic enzymes. The guts of herbivores contain many symbiotic microorganisms that have evolved to hydrolyze plant lignocellulose. Chinese bamboo rats mainly consume high-fiber foods, indicating that some members of the intestinal tract microbiota digest lignocellulose, providing these rats with the energy required for growth.ResultsHere, we used metagenomics to analyze the diversity and functions of the gut microbiota in Chinese bamboo rats. We identified abundant populations of lignocellulose-degrading bacteria, whose main functions involved carbohydrate, amino acid, and nucleic acid metabolism. We also found 587 carbohydrate-active enzyme genes belonging to different families, including 7 carbohydrate esterase families and 21 glycoside hydrolase families. The glycoside hydrolase 3, glycoside hydrolase 1, glycoside hydrolase 43, carbohydrate esterase 4, carbohydrate esterase 1, and carbohydrate esterase 3 families demonstrated outstanding performance.ConclusionsThe microbes and enzymes identified in our study expand the existing arsenal of proficient degraders and enzymes for lignocellulosic biofuel production. This study also describes a powerful approach for targeting gut microbes and enzymes in numerous industries.How to cite: Bai D, Lin X, Hu Y, et al. Metagenomics approach to identify lignocellulose-degrading enzymes in the gut microbiota of the Chinese bamboo rat cecum. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.001  相似文献   

Cd胁迫对水稻根际和非根际土壤酶活性的影响研究     

杨良静  何俊瑜  张兴平 《六盘水师范高等专科学校学报》2015,(4)

通过根袋法盆栽实验,研究了重金属Cd胁迫对水稻根际和非根际土壤酶活性的影响。结果表明,随着Cd浓度的增加,根际和非根际土壤呼吸强度增加,但增幅变小,根际呼吸强度大于非根际且差异显著。在梯度浓度的Cd胁迫下,根际和非根际脲酶、蔗糖酶活性随Cd浓度增加均表现出先升后降的趋势;5mg kg-1施Cd处理,根际和非根际的脲酶、蔗糖酶活性最强,在Cd浓度为30mg kg-1时,根际和非根际土壤中蔗糖酶、过氧化氢酶、非根际脲酶活性受到抑制,但抑制率各不相同;三种酶在根际土壤中的活性普遍高于非根际土壤;土壤酶对Cd污染的敏感度依次为蔗糖酶＞脲酶＞过氧化氢酶,蔗糖酶、脲酶可作检测土壤Cd污染的指示酶。低浓度Cd刺激使根系更加活力旺盛,根系形态指标对Cd胁迫敏感顺序为：根尖数＞根体积＞根表面积＞根长＞平均直径。  相似文献   

引流袋更换与否和导尿管相关尿路感染的临床分析     

李丽琼 《大理师专学报》2014,(10):77-79

目的：探讨引流袋更换与否和导尿管相关尿路感染的关系,减少留置导尿管相关尿路感染的发生率。方法：对留置导尿管的患者和（或）家属讲解该科研的目的和方法,完全尊重患者和家属的选择入组,观察组不更换引流袋,对照组严格按医嘱更换引流袋。两组患者留置导尿管7 d,在无菌操作下采集尿标本送细菌培养。结果：观察组与对照组感染率比较,差异有统计学意义（P＜0.05）。结论：不更换引流袋可减少导尿管相关尿路感染发生率。  相似文献   

前列腺增生症经尿道前列腺电切术并尿失禁原因及预防     

闫家文  李峰  伍松合  郑仿  赵书晓 《大众科技》2014,(10):133-134

目的：探讨良性前列腺增生症经尿道前列腺电切术后并尿失禁的原因。方法翻阅病案资料,随机选取2011年1月-2013年1月广西中医药大学第一附属医院良性前列腺增生症行前列腺电切术病人330例。术后回访6个月-1年,平均回访8个月。结果共出现尿失禁21人,发生比例为6.3%。其中,轻度尿失禁4.5%,压力性尿失禁1.2%,完全性尿失禁0.6%。结论经尿道前列腺电切术较为大多数病人所接受,是治疗良性前列腺增生症的理想方法。术后尿失禁是一种多因素综合作用的结果,部分尿失禁是由于医源性造成,需引起重视,大多数的病人可以在短时间内恢复。  相似文献   

递增负荷运动引起运动性血红蛋白低下的特点及机制     

林文弢  徐国琴  翁锡全 《北京体育大学学报》2011,34(5):52-54,59

目的:为探讨运动性血红蛋白低下时机体铁代谢、红细胞破坏与血红蛋白下降间的关系,为运动性血红蛋白低下的发生机制及预防提供依据。方法:随机选取广州体育学院体育教育系学生30人,男女各15人,进行起始负荷为60%最大心率强度,每周强度递增10%的跑步运动,5d/周,4周后测试受试者Hb、Ret、SF、Fer、sTfR和红细胞抗氧化指标MDA、SOD、CAT、GSH-px。结果:4周递增负荷跑步运动后受试者呈现运动性血红蛋白低下,血清铁蛋白持续性下降,转铁蛋白受体呈下降趋势或不变,但血清铁无明显变化;红细胞抗氧化酶的含量升高,脂质过氧化产物MDA呈持续增加趋势。结论:4周的递增负荷跑步运动中,红细胞自由基的生成增加及红细胞抗氧化能力的相对降低引起的红细胞破坏增加可能是运动性血红蛋白低下的发生原因,而不是由单纯缺铁所致。  相似文献