Induction of diabetes by Streptozotocin in rats   总被引：1，自引：0，他引：1  

A. Akbarzadeh  D. Norouzian  M. R. Mehrabi  Sh. Jamshidi  A. Farhangi  A. Allah Verdi  S. M. A. Mofidian  B. Lame Rad 《Indian journal of clinical biochemistry : IJCB》2007,22(2):60-64

The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats, makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases.  相似文献   

Study of Resistin gene expression in peripheral blood mononuclear cell and its gene polymorphism in a small range population     

Yang YM  Xu ZR  Wu LJ  Huang WD 《Journal of Zhejiang University. Science. B》2007,8(2):132-135

INTRODUCTION Recently discovered as an endocrine organ, adi-pose tissue can secrete many cytokines such as Re-sistin, adiponectin and free fatty acids, and receives much attention in the study of insulin resistance and type 2 diabetes mellitus (T2DM). As a peptide hor-mone secreted by adipocyte, Resistin is considered to be the linkage between obesity and insulin resistance (Steppan et al., 2001). Most studies on Resistin in-vestigate adipose tissue. In this experiment we de-tected t…  相似文献   

周围神经图像增强算法研究     

周学礼 《常熟理工学院学报》2011,25(4):98-101

周围神经CT图像增强是周围神经三维重建中的基础环节,本文在分析高斯圆模型Hessian矩阵特征值特点的基础上,提出一种基于Hessian矩阵多尺度滤波的周围神经CT图像增强算法.实验证明该方法能够使似圆形周围神经区域得以增强,抑止其他非圆形区域的干扰,得到较好的周围神经增强图像,为后续的分割与三维重建奠定基础.  相似文献   

晶体硅太阳电池接触电阻测量     

洪泰吾  艾斌 《三门峡职业技术学院学报》2011,10(2):106-108

金属电极与硅的接触电阻是太阳电池中串联电阻的一部分,是影响太阳电池填充因子和短路电流进而影响光电转换效率的重要因素之一,通过了解接触电阻的大小,可以判断太阳电池电极的优劣。接触电阻的测量,较为困难,难以精确,笔者给出了一种基于PN Vinod三点测试方法的改进方法及测试实例。  相似文献   

集训对舞龙队女大学生血常规及内皮祖细胞的影响     

孔明涯  任路遥 《体育科技文献通报》2020,(4):156-158

目的:分析舞龙运动对女大学生血常规及内皮祖细胞含量的影响。方法:选取2017级舞龙队女大学生10人为训练组,2017级普通女大学生10人作为对照组。舞龙队女大学生备战比赛进行连续4周,每周3次,每次90min的赛前集训,普通女大学生除日常活动外,没有额外进行任何其他运动队的集训。集训结束后,在受试者都处于空腹状态下对两组受试者进行静脉采血并进行血常规、早期内皮祖细胞和晚期内皮祖细胞的检测。所得数据进行统计学分析。结果:参加舞龙集训的女大学生的血常规指标与普通女大学生的血常规指标无显著差异,早期EPCs和晚期EPCs无显著性差异,但在数值上显示出不同的趋势。结论:参加舞龙集训的女大学生身体处于健康状态。参加舞龙集训有可能增强运动员血管的自我修复能力。  相似文献   

Blood flow restriction in human skeletal muscle during rest periods after high-load resistance training down-regulates miR-206 and induces Pax7     

Ferenc Torma  Zoltan Gombos  Marcell Fridvalszki  Gergely Langmar  Zsofia Tarcza  Bela Merkely  Hisashi Naito  Noriko Ichinoseki-Sekine  Masaki Takeda  Zsolt Murlasits  Peter Osvath  Zsolt Radak 《运动与健康科学(英文)》2021,10(4):470-477

BackgroudBlood flow restriction (BFR) with low-intensity resistance training has been shown to result in hypertrophy of skeletal muscle. In this study, we tested the hypothesis that BFR during the rest periods between acute, high-intensity resistance exercise sessions (70% of 1 repetition maximum, 7 sets with 10 repetitions) enhances the effects of the resistance training.MethodsA total of 7 healthy young men performed squats, and between sets BFR was carried out on one leg while the other leg served as a control. Because BFR was applied during rest periods, even severe occlusion pressure (approximately 230 mmHg), which almost completely blocked blood flow, was well-tolerated by the participants. Five muscle-specific microRNAs were measured from the biopsy samples, which were taken 2 h after the acute training.ResultsDoppler data showed that the pattern of blood flow recovery changed significantly between the first and last BFR. microRNA-206 levels significantly decreased in the BFR leg compared to the control. The mRNA levels of RAC-β serine/threonine-protein kinase v22, nuclear respiratory factor 1, vascular endothelial growth factor, lupus Ku autoantigen protein p70 genes (p < 0.05), and paired box 7 (p < 0.01) increased in the BFR leg. The protein levels of paired box 7, nuclear respiratory factor 1, and peroxisome proliferator-activated receptor γ coactivator 1α did not differ between the BFR leg and the control leg.ConclusionBFR, during the rest periods of high-load resistance training, could lead to mRNA elevation of those proteins that regulate angiogenesis, mitochondrial biogenesis, and muscle hypertrophy and repair. However, BFR also can cause DNA damage, judging from the increase in mRNA levels of lupus Ku autoantigen protein p70.  相似文献   

罗氏沼虾眼柄神经分泌细胞的超微结构   总被引：6，自引：0，他引：6  

姚泊 《中山大学学报论丛》1995,(3)

对产卵前罗氏沼虾眼柄的神经分泌细胞胞质，能观察到高尔基体和线粒体等细胞器结构，在高尔基体的生成面和成熟面均有许多高尔基小泡，核膜仅在部份区域有核周腔增宽现象，对产卵后罗氏沼虾眼柄的神经分泌细胞胞质，能观察到线粒体和许多大小不一的泡状结构，但没有观察到高尔基体，核膜都有明显的和不同程度的核周腔增宽现象．  相似文献   

神经营养素家族类细胞因子对神经干细胞分化影响的研究现状     

张任飞  肖诗柔 《中国科技信息》2011,(2):176-178

神经干细胞（NSCs）是具有高度自我更新能力并能分化为神经元、星形胶质细胞和少突胶质细胞的神经前体细胞,具有广阔的临床应用前景。神经营养素家族对神经干细胞的分化有一定影响。其包括：神经生长因子（NGF）、脑源性神经生长因子（BDNF）、神经营养素-3（NT-3）、神经营养素-4/5（NT-4/5）以及神经营养素-6（NT-6）。本文就目前神经营养素家族对NSCs分化的影响的研究现状进行综述。  相似文献   

间歇性低氧训练对耐力能力的影响     

施朝阳 《湖北体育科技》2009,28(5):560-561

分析间歇性低氧训练的应用机制及对人体的运动能力特别是红细胞的影响因素;分别论述它们对运动员的携氧能力的影响,以求在训练方法上有所创新和突破。研究结果表明：运动员通过间歇型低氧训练红细胞数量增多,其耐力能力明显提高。  相似文献   

优秀田径运动员心率变异性分析   总被引：4，自引：0，他引：4  

黄晖明  朱晓梅  刘凌 《体育与科学》2008,29(6)

目的:分析优秀田径运动员心率变异性(HRV)特征,为HRV在运动员选材、机能评定等应用提供理论依据。方法:选取江苏省专业运动队优秀田径运动员和普通健康人各25名进行连续5min心电图时域和频域记录获得HRV指标,以普通健康人为对照分析优秀田径运动员HRV特征。结果:(1)女运动员VLF低于男运动员(P<0.05),其余指标无性别差异。(2)优秀田径运动员SDNN、RMSSD、PNN50、TP、HF、VLF均高于普通健康人(P<0.05或P<0.01);LF、LF/HF二者无差异(P>0.05)。结论:(1)优秀田径运动员自主神经活性和迷走神经的紧张性没有性别差异,安静时男性运动员拥有较高的交感神经张力。(2)优秀田径运动员安静时比普通健康人拥有更强的心脏自主神经活性、更高的迷走神经紧张性和交感神经兴奋性,迷走和交感神经均衡性无差异。  相似文献