微生物燃料电池在环境工程专业教学中的应用     

张宝刚  杨广元  胡伟武  冯传平 《中国地质教育》2013,22(1):93-96

模型案例具有形象化与趣味性的特点,在环境工程教学中历来占有重要位置。微生物燃料电池是近年来新兴并迅速发展的一种环境保护技术,可以在处理污染物的同时回收能源,具有良好的发展前景。该技术可以作为案例引入到环境工程专业教学中。从环境工程主要知识点、新型案例教学设计和课堂实践等方面介绍了其与环境工程专业教学的密切联系,展示环境工程领域最新的发展动向,以此激发学生学习专业课的热情,培养学生的研究能力与创新意识。  相似文献   

Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats     

Izatus Shima Taib  Siti Balkis Budin  Seri Maseran Siti Nor Ain  Jamaludin Mohamed  Santhana Raj Louis  Srijit Das  Sulaiman Sallehudin  Nor Fadilah Rajab  Othman Hidayatulfathi 《Journal of Zhejiang University. Science. B》2009,10(11):813-819

Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, che-mopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat's red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinoeyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.  相似文献   

Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4＋ CD25＋ Treg cells   总被引：5，自引：0，他引：5  

Hang Yan  Chen-guang Ding  Pu-xun Tian  Guan-qun Ge  Zhan-kui Jin  Li-ning Jia  Xiao-ming Ding  Xiao-ming Pan  Wu-jun Xue 《Journal of Zhejiang University. Science. B》2009,10(12):928-932

Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4~+ CD25~+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4~+ CD25~+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4~+ CD25~+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4~+ CD25~+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4~+ CD25~+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4~+ CD25~+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies.  相似文献   

高原地区中长跑运动员心率变异性特征     

宋淑华  高春刚 《北京体育大学学报》2012,35(4):70-73

目的:了解高原地区中长跑运动员心率变异性特征。方法:云南师范大学男子中长跑运动员12名,设为实验组,普通大学生12名,设为对照组。对两组安静状态下10 min短时心率变异性进行时域、频域及非线性分析。结果:实验组时域各项指标平均RR、RMSSD、pNN50、SDNN/HR均显著高于对照组(P0.05,P0.01)。频域各指标中HFn.u.显著高于对照组(P0.05),而LFn.u.显著低于对照组(P0.05),最终LF/HF比值极显著低于对照组(P0.01)。结论:和高原地区普通大学生相比,中长跑运动员具有较高的心率变异性和较大的自主神经调节能力,且与平原地区相应人群无明显差异。  相似文献   

运动训练对大鼠主动脉一氧化氮合酶及细胞凋亡的影响   总被引：2，自引：4，他引：2  

田振军  吴潇男 《天津体育学院学报》2007,22(1):6-8

目的:探讨不同强度训练对大鼠主动脉一氧化氮合酶(NOS)及细胞凋亡的影响。方法:采用跑台训练方式,建立大鼠有氧运动和疲劳运动模型,运用免疫组织化学SABC法研究不同运动强度对大鼠主动脉神经型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)、诱导型一氧化氮合酶(in-duced nitric oxide synthase,iNOS)、内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)和细胞凋亡蛋白(Bcl-2/Bax)表达的影响。结果:有氧训练组与对照组比较,主动脉NOS 3个亚型及Bcl-2,Bax表达差异显著(P<0.05);疲劳训练组与对照组和有氧训练组比较,主动脉iNOS、eNOS及Bcl-2,Bax表达差异显著(P<0.05),nNOS虽有增加但差异不显著。结论:运动可引起大鼠主动脉NOS 3个亚型及Bcl-2,Bax表达变化,且与运动强度关系密切。有氧训练可使主动脉NOS适度表达且抑制细胞凋亡,疲劳训练会导致大鼠主动脉iNOS过量表达,细胞发生凋亡现象。因此认为,运动训练引起NOS亚型表达变化与运动诱导的主动脉细胞凋亡关系密切。  相似文献   

消化性溃疡病患者胃窦粘膜幽门腺5-HT细胞和G细胞免疫组织化学研究     

王建伟  周济远  高增贺  任君旭  郑慧娥  白雪梅 《河北北方学院学报(医学版)》1996,(2)

胃溃疡(Gu)、十二指肠溃疡(Du)和对照组(Con)各5例,取肉眼下非病变区胃窦粘膜,用免疫组织化学PAP双重染色法,在同一张切片上显示5-HT细胞和G细胞。结果5-HT细胞多散在于粘膜的中下1/3,呈棕褐色,形状多样,常见有突起的细胞。G细胞呈带状分布于粘膜的中1/3,大部分G细胞为蓝色,个别细胞为蓝黄混合色。它们多为圆形或锥体形。有时可见5-HT细胞和G细胞相毗邻。5-HT细胞数和G细胞数在Gu组分别为:4.17±1.21、7.87±3.13;Du组:5.45±1.96、9.21±5.72;Con组:3.21±1.64、6.19±1.72。5-HT细胞数和G细胞数在Gu组和Du组都增加。且都以Du组为最多,经相关分析二者呈正相关关系。Gu和Du组的5-HT细胞数和G细胞数与对照组相比均有高度显著性差异。Du组的5-HT细胞数和G细胞数与Gu组相比较,亦有高度显著性差异。提示5-HT细胞和G细胞可能参与了消化性溃疡病的调节,特别与Du的关系更为密切。5-HT细胞和G细胞之间的关系有待于进一步研究。  相似文献   

大鼠生后发育期间胰岛胰岛素细胞免疫组织化学研究     

潘秀芳  穆金秀  石爱荣  崔铮 《河北北方学院学报(医学版)》1993,(3)

用免疫组织化学 PAP 法,显示胰岛 B 细胞,观察大鼠生后发育期间全胰胰岛 B 细胞的形态、分布和数量变化。结果表明,B 细胞多呈园形或椭园形。B 细胞多位于胰岛中央,极少数 B 细胞位于胰腺外分泌部或导管上皮内。B 细胞百分数于1d 组最低,28d 组最高。结果提示,大鼠生后发育期间胰岛 B 细胞在继续发育。B 细胞具有其内胚层起源的形态学基础。  相似文献   

新生儿脐血细胞检测分析     

尹卫东  刘克芹  刘晨  胡晓燕  商万军  李红丽 《河北北方学院学报(医学版)》2001,18(6):3-5

目的探讨新生儿脐静脉血细胞的特点,为新生儿脐血细胞参考值调查及新生儿健康情况提供资料.方法用Sysmex K-4500全自动血细胞分析仪检测51例脐静脉血及50例成人静脉血,并作统计分析,性别间、组间比较采用均数的t检验.结果发现新生儿脐血细胞测定值不同于新生儿末梢血测定值,在男女间MCV、PLT差异有极显著性意义(P＜0.01),RDW差异有显著性意义(P＜0.05);此外,脐血细胞PDW、MPVR、及P-LCR在剖宫产组、胎吸组与顺产组的差异有极显著性意义(P＜0.01).结论新生儿脐血细胞参数是一组特殊资料,反映了新生儿在宫内的发育状况,并与不同分娩方式有关.  相似文献   

在EXCEL中使用VBA编程处理数据   总被引：4，自引：0，他引：4  

王强 《福建教育学院学报》2003,4(10):84-87

本文通过利用EXCEL中的VBA编写宏来自动生成全国等级考试机试安排表的实例 ,说明EXCEL中宏的应用和单元格引用的方法。  相似文献   

Nonlinear modelling of a SOFC stack by improved neural networks identification     

WU Xiao-juan ZHU Xin-jian CAO Guang-yi TU Heng-yong 《浙江大学学报(A卷英文版)》2007,8(9):1505-1509

The solid oxide fuel cell (SOFC) is a nonlinear system that is hard to model by conventional methods. So far,most existing models are based on conversion laws,which are too complicated to be applied to design a control system. To facilitate a valid control strategy design,this paper tries to avoid the internal complexities and presents a modelling study of SOFC per-formance by using a radial basis function (RBF) neural network based on a genetic algorithm (GA). During the process of mod-elling,the GA aims to optimize the parameters of RBF neural networks and the optimum values are regarded as the initial values of the RBF neural network parameters. The validity and accuracy of modelling are tested by simulations,whose results reveal that it is feasible to establish the model of SOFC stack by using RBF neural networks identification based on the GA. Furthermore,it is possible to design an online controller of a SOFC stack based on this GA-RBF neural network identification model.  相似文献