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31.
Shao-xiang Weng Mei-hua Sui Shan Chen Jian-an Wang Geng Xu Ji Ma Jiang Shan Lu Fang 《Journal of Zhejiang University. Science. B》2009,10(7):528-535
Objective This study is to determine the effect of the natural product parthenolide, a sesquiterpene lactone isolated from extracts
of the herb Tanacetum parthenium, on the proliferation of vascular smooth muscle cells (VSMCs).
Methods Rat aortic VSMCs were isolated and cultured in vitro, and treated with different concentrations of parthenolide (10, 20 and
30 μmol/L). [3H]thymidine incorporation was used as an index of cell proliferation. Cell cycle progression and distribution were determined
by flow cytometric analysis. Furthermore, the expression of several regulatory proteins relevant to VSMC proliferation including
IκBα, cyclooxygenase-2 (Cox-2), p21, and p27 was examined to investigate the potential molecular mechanism.
Results Treatment with parthenolide significantly decreased the [3H]thymidine incorporation into DNA by 30%∼56% relative to control values in a dose-dependent manner (P<0.05). Addition of parthenolide also increased cell population at G0/G1 phase by 19.2%∼65.7% (P<0.05) and decreased cell population at S phase by 50.7%∼84.8% (P<0.05), which is consistent with its stimulatory effects on p21 and p27. In addition, parthenolide also increased IκBα expression
and reduced Cox-2 expression in a time-dependent manner.
Conclusion Our results show that parthenolide significantly inhibits the VSMC proliferation by inducing G0/G1 cell cycle arrest. IκBα and Cox-2 are likely involved in such inhibitory effect of parthenolide on VSMC proliferation. These
findings warrant further investigation on potential therapeutic implications of parthenolide on VSMC proliferation in vivo.
Project (No. 491020-W50315) supported by the Foundation of the Health Bureau of Zhejiang, China 相似文献
32.
细胞增殖是细胞生命活动的重要特征之一.物质准备和细胞分裂是一个相互联系的过程,这一过程即为细胞增殖.细胞增殖是生物繁育的基础.成体生物也需要细胞增殖,以弥补代谢过程中的细胞损失.而细胞增殖受到严密的调控机制所监控,遵循一定的规律,此过程中任何一个关键步骤的错误都有可能导致严重后果,乃至死亡.因此,细胞增殖调控是整个生命活动的最基本保证. 相似文献
33.
为研究AngⅡ对3T3-L1前脂肪细胞增殖的影响,本文采用血清法和血清饥饿法两种方法,外源添加不同浓度AngⅡ,利用MTT法检测3T3-L1增值情况。研究结果表明,有血清法和血清饥饿法中AngⅡ对3T3-L1前脂肪细胞的增殖均无显著性影响（p〉0．05）。 相似文献
34.
35.
生化分析中常用的惰性载体——琼脂糖 总被引：1，自引：0，他引：1
琼脂糖作为优良的惰性载体和高分子筛在近代生物化学的定性、定量分析中,在对微生物蛋白质等生物材料的分离技术中得到广泛的应用。本文对琼脂糖在平板电流法、免疫扩散法、免疫电泳法、凝胶过滤法、亲合层洗法等生物技术中的应用,以及对如何在实验室中从琼脂中除去琼脂果胶制取琼脂糖做了论述。 相似文献
36.
Yi-Sub Kwak 《Journal of sports sciences》2013,31(9):973-978
Abstract The purpose of this study was to analyse the effect of regular exercise on spleen and peritoneal exudate reactive oxygen species (ROS) and lymphocyte proliferation by splenocytes. Twenty-four female BALB/c mice were randomly divided into trained (n = 12) and untrained (n = 12) groups. These two groups were further divided into mice that were studied at rest (trained/rest, n = 5; untrained/rest, n = 6) and immediately after a 2 h acute bout of exercise (trained/exercise, n = 6; untrained/exercise, n = 6). The animals were bred in the animal facility of the Yonsei University College of Medicine, where they were housed in a temperature- (22 – 24°C) and humidity- (50 – 60%) controlled environment, with a 12 h photoperiod, and provided with food and water ad libitum. The trained mice underwent 10 weeks of endurance swimming training (5 days per week) in water at 26 – 29°C for 60 min. Changes in body mass, proliferative activity and the production of reactive oxygen species from spleen lymphocytes and peritoneal exudate cells were determined. The splenic lymphocytes of the trained mice had much greater proliferative activity than those of the untrained mice (P < 0.05). Trained mice had lower ROS production in splenic lymphocytes and peritoneal exudate cells than untrained mice. In both groups, there was substantial inhibition of proliferative activity stimulated with medium, concanavalin A and lipopolysaccharide following the acute bout of exercise. This may have been caused by excessive ROS production following the acute exercise session. 相似文献
37.
Emad Y. Moawad 《Indian journal of clinical biochemistry : IJCB》2014,29(4):442-451
The purpose of this study is optimizing the l-arginine (l-Arg) doses on the basis of chemical structure in regional accessible tumor therapy to settle down a new protocol for the treatment of cancer. 3H-thymidine-based cell proliferation assay was performed in vitro on tumor cell lines of fibrosarcoma (FS), lymphosarcoma-ascitic and on normal cell line of NIH 3T3 after treatment with different concentrations of l-Arg in phosphate buffered saline (PBS). The cultures were harvested after 22 h and the incorporated radioactivity was counted to identify their histologic grades as described in earlier studies. In vivo therapy of murine tumors was conducted where FS cells injected subcutaneously at ventro-lateral position of mice. Various drug delivery schedules were injected into the centre of tumor base, once a day for 4 days. Tumor diameter and survivals were monitored where the day of sacrifice was considered for monitoring the survival period. By identifying the histologic grades of the treated cultures in vitro and in vivo by different concentrations of l-Arg, the corresponding energy of such concentrations were determined. An efficient model with a good fit (R2 = 0.98) was established to describe the energy yield by l-Arg dose. The equivalence between the tumor histologic grade and energy of the l-Arg dose delivered in saline (PBS) environment is the optimum condition for regional tumor therapy achieves higher survival rate. The selective cytotoxicity to tumor cells with minimal damage to normal cells by l-Arg due to its chemical structure suggests to be considered the most promising drug for regional therapy of the accessible tumors like breast cancers of early stage with no distant metastasis. 相似文献
38.
目的探讨厚朴酚对人视网膜母细胞瘤HXO-RB44生长增殖的影响。方法体外培养HXO-RB44细胞,用终浓度为5～40μM的厚朴酚处理24～72h,用MTT法检测厚朴酚对细胞生长抑制情况,并用流式细胞术检测处理后细胞周期的改变。结果 5～40μM的厚朴酚能抑制HXO-RB44细胞的生长,随着浓度的增加,抑制效应逐渐增强。同时,厚朴酚随着孵育时间的延长,抑制效应更加显著。流式细胞术证实厚朴酚能将HXO-RB44细胞抑制在S期。结论厚朴酚能体外抑制HXO-RB44细胞的增殖,是通过将细胞阻滞在S期而发挥抑制作用。 相似文献
39.
运动量及年龄因素对淋巴细胞转化的影响 总被引：3，自引：2，他引：1
有关运动后淋巴细胞对丝裂原的增殖反应性变化 ,在运动与免疫研究领域有许多矛盾的报道 ,通过研究不同运动及年龄因素对淋巴细胞增殖反应的变化 ,结果表明 :力竭游泳使淋巴细胞对 L PS的增殖反应减低 ,因此可能会损害与 B细胞相联系的免疫功能。力竭游泳似乎对 T细胞的转化活性影响尚不确定 ,与运动量有关 ,亦与实验对象的年龄有关 ;不同运动量的运动对 T细胞转化的影响不同 ,似乎运动对 T细胞转化的影响缺乏渐变性。脾细胞比肠淋巴结细胞对 Con A诱导的增殖反应强烈 ,但二者的变化趋势相同 (r =0 .75 ,P <0 .0 1) 相似文献
40.
Shao-xiang WENG Mei-hua SUI Shan CHEN Jian-an WANG Geng XU Ji MA Jiang SHAN Lu FANG 《Journal of Zhejiang University. Science. B》2009,10(7)
Objective: This study is to determine the effect of the natural product parthenolide, a sesquiterpene lactone isolated from extracts of the herb Tanacetum parthenium, on the proliferation of vascular smooth muscle cells (VSMCs). Methods: Rat aortic VSMCs were isolated and cultured in vitro, and treated with different concentrations of parthenolide (10, 20 and 30 μmol/L).[3H]thymidine incorporation was used as an index of cell proliferation. Cell cycle progression and distribution were determined by flow cytometric analysis. Furthermore, the expression of several regulatory proteins relevant to VSMC proliferation including IκBα, cyclooxygenase-2 (Cox-2), p21, and p27 was examined to investigate the potential molecular mechanism. Results:Treatment with parthenolide significantly decreased the [3H]thymidine incorporation into DNA by 30%～56% relative to control values in a dose-dependent manner (P＜0.05). Addition of parthenolide also increased cell population at G0/G1 phase by 19.2%～65.7% (P＜0.05) and decreased cell population at S phase by 50.7%～84.8% (P＜0.05), which is consistent with its stimulatory effects on p21 and p27. In addition, parthenolide also increased IκBα expression and reduced Cox-2 expression in a time-dependent manner. Conclusion: Our results show that parthenolide significantly inhibits the VSMC proliferation by inducing G0/G1 cell cycle arrest. IκBα and Cox-2 are likely involved in such inhibitory effect of parthenolide on VSMC proliferation. These findings warrant further investigation on potential therapeutic implications of parthenolide on VSMC proliferation in vivo. 相似文献