HPLC法测定猪血浆中土霉素     

宋九华  严军  万东海 《乐山师范学院学报》2007,22(5):29-30

本文研究和发展了高灵敏度测血浆中土霉素含量的HPLC方法.用VP-ODS(5um,150×4.6 mm)色谱柱,以乙腈:PBS=20:80(PBS:0.05mol/LK2HPO4、0.01mol/LEDTA、30mmol/L三乙胺再用HCl调节溶液pH为6.5该溶液即为PBS溶液)为流动相,流动相流速1mL/min,以353nm为检测波长.土霉素在0.01ug/mL～3.00ug/mL范围内呈良好的线性关系,检出限为0.01ug/mL,平均回收率为97.28%,平均相对标准偏差为1.98%.本方法简便,分离度高,重现性好,检出限低,适用于血浆中土霉素检测和含量测定.  相似文献   

澳特斯小儿止咳露对儿童支气管哮喘发作时ET、TNF-α水平变化观察     

黄芬  陈新宙  黄新华 《宜春学院学报》2006,28(4):93-94

目的:观察澳特斯小儿止咳露对儿童支气管哮喘发作时ET、TNF-α水平变化.方法:将78例支气管哮喘患儿随机分为二组,在按照儿童支气管哮喘防治常规的治疗原则的同时,治疗组口服澳特斯小儿止咳露,对照组未提供口服止咳药物;并在治疗前和治疗后48小时进行监测血桨内皮素(ET)、肿瘤坏死因子(TNF-α)浓度.结果:治疗前后血浆ET、TNF-α比较,治疗组存在显著差异(P<0.05),而对照组无显著差异(P>0.05).结论:澳特斯与干扰血浆ET、TNF-α浓度有关.  相似文献   

Intestinal microflora in rats with ischemia／reperfusion liver injury     

Xing HC  Li LJ  Xu KJ  Shen T  Chen YB  Sheng JF  Yu YS  Chen YG 《Journal of Zhejiang University. Science. B》2005,6(1):14-21

Objectives:To investigate the intestinal microflora status related to ischemia/reperfusion(I/R)liver injury and explorethe possible mechanism.Methods:Specific pathogen free grade Sprague-Dawley rats were randomized into three groups:Controlgroup(n=8),sham group(n=6)and I/R group(n=10).Rats in the control group did not receive any treatment,rats in the I/R groupwere subjected to 20 min of liver ischemia,and rats in the sham group were only subjected to sham operation.Twenty-two hourslater,the rats were sacrificed and liver enzymes and malondialdehyde(MDA),superoxide dismutase(SOD),serum endotoxin,intestinal bacterial counts,intestinal mucosal histology,bacterial translocation to mesenteric lymph nodes,liver,spleen,andkidney were studied.Results:Ischemia/reperfusion increased liver enzymes,MDA,decreased SOD,and was associated withplasma endotoxin elevation in the I/R group campared to those in the sham group.Intestinal Bifidobacteria and Lactobacillidecreased and intestinal Enterobacterium and Enterococcus  相似文献   

聚明胶肽注射液鲎试剂法检测细菌内毒素的研究     

王海雄 《科技风》2010,(7)

目的:建立快速的聚明胶肽注射液细菌内毒素检查法,从而取代热原检查法。方法:按中国药典2005年版二部附录所载的细菌内毒素检查法进行实验。结果:将聚明胶肽注射液稀释16倍可消除干扰作用,用标示灵敏度为0.03Eu.mL-1的检测细菌内毒素有效,结果准确可靠。结论:利用细菌内毒素检查法代替聚明胶肽注射液热原检查是可行的。  相似文献   

血浆中粉防己碱和防己诺林碱提取方法的比较研究     

杨彩玲  陈宏彬  张鸣 《甘肃高师学报》2009,14(2)

利用液相微萃取(LPME)方法对人血浆中粉防己碱和防己诺林碱进行提取,重点考察了液相微萃取的最优条件,并从提取效率、净化能力、提取成本和绿色环保等方面分别与液-液萃取、固相萃取和蛋白质沉淀的方法进行了比较.  相似文献   

血浆置换术治疗特发性血小板减少性紫癜20例护理体会     

廖九女 《宜春学院学报》2009,31(4):67-68

目的：探讨血浆置换术治疗特发性血小板减少性紫殿患者的护理,为临床护理提供临床经验。方法：通过对20例血浆置换术治疗特发性血小板减少性紫殿患者的护理,总结其护理过程和注意事项。结果：20例中有18例治愈,置换次数为3-15次,2例治疗无效死亡。结论：术前了解病情,取得病人及家属合作,做好充分的准备;术中确保有效的循环通路;术后密切观察病情变化,是PE成功的基础和关键。  相似文献   

Determination of 5-Fluorouracil in Human Plasma by High- Performance Liquid Chromatography （HPLC     

谷元  陆榕  司端运  刘昌孝 《天津大学学报(英文版)》2010,16(3):167-173

5-Fluorouracil (5-FU) has a broad spectrum of anti-tumor activity, widely applied to the treatment of cancers. However, it is necessary to determine the plasma concentration of 5-FU in clinical practice due to its narrow therapeutic index. Therefore, a simple, economic and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for the determination of 5-FU in human plasma. Ethyl acetate was chosen as extraction reagent. Chromatographic separation was performed on a Diamonsil C18 column (250 mm × 4.6 mm i.d., 5 μm) with the mobile phase consisting of methanol and 20 mmol/L ammonium formate using a linear gradient elution at a flow rate of 0.8 mL/min. 5-FU and 5-bromouracil (5-BU) were detected by UV detector at 265 nm. The calibration curve was linear over the concentration range of 5—500 ng/mL and the correlation coefficient was not less than 0.992 6 for all calibration curves. The intra- and inter-day precisions were less than 10.5% and 4.3%, respectively, and the accuracy was within ±3.7%. The recovery at all concentration levels was 80.1±8.6%. 5-FU was stable under possible conditions of storing and handling. This method is proved applicable to therapeutic drug monitoring and pharmacokinetic studies of 5-FU in human.  相似文献   

血浆置换的护理配合     

尹爱华 《赤峰学院学报(自然科学版)》2013,(23):136-136

通过血浆置换术,清除患者体内各种毒素和致病因子,从而达到治疗的目的,保证血浆置换的顺利进行,护理配合是血浆置换术成功的关键．  相似文献   

注射用碟脉酮细菌内毒素检查方法研究     

彭禹 《今日科苑》2008,(4):86

目的是建立注射用碟脉酮细菌内毒素的检查法。方法采用《中国药典》2000年版附录细菌内毒素检查法。结果注射用碟脉酮经稀释后,对不同厂家、不同灵敏度的鲎试剂表现或抑制、或增强、或无干扰。得出结论,注射用碟脉酮不适合用鲎试剂进行细菌内毒素检查。  相似文献   

头孢尼西钠细菌内毒素检查法研究     

孙秀敏  高媛  王广慧 《黑龙江科技信息》2008,(3):175-175

目的:建立头孢尼西钠细菌内毒素检查法.方法:按<中国药典>2005年版二部附录Ⅺ E进行试验和结果判断.结果:用两个不同生产厂家,不同标示灵敏度鲎试剂进行研究,头孢尼西钠的最大不干扰浓度为3.2mg/ml,并对3批样品进行检查,均无干扰作用,其细菌内毒素均小于0.15EU/mg.结论:本品细菌内毒素检查法(凝胶法)控制细菌内毒素的量合理可靠.  相似文献