金钗石斛愈伤组织培养     

李庆宏  宋常美 《贵阳金筑大学学报》2011,(3):76-79

本文研究了基本培养基和植物生长调节剂对金钗石斛愈伤组织诱导和增殖的影响,并绘制了生长曲线。结果表明,改良MS2（K2HPO．加倍）能获得60％的诱导率,而改良MS。CNH4NO,加倍）的诱导率仅为5％。二者在愈伤组织培养上也表现出显著差异,前者表现良好,说明高浓度的铵能抑制金钗石斛愈伤组织诱导和增殖。MS＋0．5mg／LBA＋O．8Ing／LNAA＋3％蔗糖可获得最高诱导率和增殖倍数,诱导率为70．0％。90d后增殖倍数为23．98。类原球茎培养60～100d时进入对数生长期,重量快速增加。  相似文献   

玉舍森林公园猕猴桃属植物多样性研究   总被引：6，自引：0，他引：6  

林长松  左经会  田应洲  向红  孙爱群 《六盘水师范高等专科学校学报》2005,17(4):1-5

通过对玉舍国家级森林公园猕猴桃属(ActinidiaLindl)植物资源的系统调查研究,发现该区分布有猕猴桃属植物8种2变种,且资源蕴藏量大。它们在食品、医药及园林等方面都有很重要的用途。本文编制了猕猴桃属植物的分种检索表,并对其叶、花、果、营养等多样性进行研究。  相似文献   

陆英中乌索酸的分离与鉴定(Ⅰ)   总被引：7，自引：3，他引：7  

李开泉  陈武  叶文峰  邹盛勤  邓庭亭  杨利萍 《宜春学院学报》2003,25(2):1-2

目的：分离和鉴定陆英中抗肝炎主要成分乌索酸．方法：采用“醉提凝析法”提取乌索酸．结果：产品经理化常数和光谱学鉴定，确征为乌索酸，其提取率达90．2％，纯度达99．72％．结论：本工艺技术具有先进合理、实用可行、有利于工业化生产的特点．  相似文献   

猕猴桃属十种三变种的染色体数目   总被引：1，自引：0，他引：1  

熊治廷  黄仁煌 《中国科学院研究生院学报》1988,26(3):245-247

Somatic chromosome numbers of 10 species and 3 varieties in  Actinidia Lindl. are reported for the first time, except A. polygama (Sieb. et Zucc.) Maxim. Two ploidy levels, diploid and tetraploid, are found both in A. polygama and A. kolomikta (Ma- xim. et Rupr.) Maxim. Chromosome numbers are listed as follows: A. kolomikta (Maxim. et Rupr.) Maxim. 2n=58, 116, A. polygama (Sieb. et Zucc. Maxim. 2n=58, 116, A. macrosper- ma Liang 2n=116, A. callosa var. henryi Maxim. 2n=116, A. callosa var. discoler Liang 2n =116, A. cylindrica Liang 2n=58, A. chrysantha Liang 2n=116, A. sabiaefolia Dunn 2n= 58, A. melliana Hand.-Mazz. 2n=58, A. hemsleyana Dunn 2n=58, A. fulvicoma var. lanata (Hemsl.) Liang 2n=58, A. latifolia (Gardn. et Champ.) Merr. 2n=58, and A. lanceolata Dunn 2n=58.  相似文献   

光叶海桐愈伤组织诱导及增殖研究     

蒋炜  苏智先  陈光登 《绵阳师范学院学报》2007,26(11):61-65

以光叶海桐的叶和茎为外植体,进行了组织培养愈伤组织诱导的实验研究。结果表明:在以MS培养基和WPM培养基为基本培养基附加不同外源激素2,4-D、NAA、KT、BA条件下,光叶海桐在一个较宽的生长范围内,均可诱导产生愈伤组织。最佳诱导和增殖条件是MS 2,4-D(1.5mg/L) BA(1.0mg/L)光、暗交替(光照14h/d)。在此条件下,30d后,叶的诱导率达90%,愈伤组织继代培养14d后,平均增殖率达198.8%。  相似文献   

不同产地金钗石斛红外光谱解析   总被引：3，自引：0，他引：3  

邓月娥  孙素琴  牛立元 《河南科技学院学报》2007,35(2):38-40

利用傅利叶变换红外光谱仪（Fourier Transform Infrared Spectroscopy）分析了来自云南和浙江的两个金钗石斛（Dendrobium nobile Lindl.）的红外光谱特征。两个金钗石斛样品均具有基本相似的红外光谱,但个别吸收峰的峰强及峰位具有明显区别。云南金钗石斛具有明显的2851 cm^-1吸收峰和较小的1736 cm^-1的吸收峰,而浙江金钗石斛具有较小的2852 cm^-1峰和很强的1740 cm^-1吸收峰,其峰高与1636 cm^-1处的峰高相当。两者的二阶导数谱在1055 cm^-1和1073 cm^-1附近的峰也有明显区别。这种方法对于快速直接判定金钗石斛的产地来说是可行的。  相似文献   

Effects of mechanical vibration on root development of Actinidia chinensis plantlet     

YANG Xiao-cheng DING Jian-ping WANG Bo-chu 《重庆大学学报(英文版)》2007,6(4):227-230

The root development of Actinidia chinensis plantlets was studied in exposure to environmental stress of mechanical vibration at respectively 1 Hz, 2 Hz, 3 Hz, 4 Hz and 5 Hz. The plantlets exposed to vibration stimuli at all those frequencies have a larger total number and a larger total length of roots and a smaller permeability of root plasma-membrane, compared with those cultivated in an environment without vibration stress. Vibration at respectively 1 Hz, 2 Hz, 3 Hz and 4 Hz enhances root activity and the 3 Hz vibration is the most favorable. There is an obvious negative correlation between root activity and permeability of root plasma-membrane. The effects may be explained by the likelihood that mechanical vibration at an appropriate frequency facilitates roots’ absorbing water and minerals which are indispensable to inducing and synthesizing in roots some active substances favorable to growth. Nevertheless, overstress damages the integrity of root plasm-membrane, increases the permeability, and results in the disability of protecting root cells.  相似文献   

GC-MS法对软枣猕猴桃中脂肪酸的成分分析     

宋美晶  侯冬岩  回瑞华  金铭 《鞍山师范学院学报》2013,(2):44-46

对软枣猕猴桃不同部位的脂肪酸组成和含量进行了分析对比.采用索氏提取法提取果肉和果籽中的脂肪油,以氢氧化钾-甲醇溶液对脂肪油中的脂肪酸进行甲酯化,用气相色谱-质谱联用仪进行分析.结果表明:软枣猕猴桃果肉和果籽中均含有亚麻酸,且果籽的亚麻酸含量高于果肉;果肉中含有亚油酸,但在果籽中并未检出.  相似文献   

Essential oil of Actinidia macrosperma, a catnip response kiwi endemic to China     

Zhao YP  Wang XY  Wang ZC  Lu Y  Fu CX  Chen SY 《Journal of Zhejiang University. Science. B》2006,7(9):708-712

Objective： To identify compounds that may be responsible for catnip response of Actinidia macrosperma, and compare chemical compositions in the wild and in vitro regenerated plants. Methods： GC-MS and relative retention indices with n-alkanes as reference points were used for compound identification, and component relative percentage was calculated based on GC peak areas without using correction factors. Results： There are 28 compounds （92.72%） and 15 compounds （93.88%） identified in the essential oils from the wild and regenerated plants, respectively. Dihydronepetalactone, iridomyrmecin, and dihydroactinidiolide, which are believed to be attractive to felines, are present in both wild and regenerated plants. Actinine was not detected, and beta-pheylethyl alcohol was only present in wild plant, in addition, short-chain enol derivatives, messengers in chemical communication, are commonly present in wild plant of A. macrosperma, but absent in regenerated one. Conclusion： Dihydronepetalactone, iridomyrmecin, and dihydroactinidiolide are responsible for the catnip response ofA. macrosperma.  相似文献