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11.
A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.  相似文献   
12.
FDP在临床中有许多积极作用,其表现为:FDP可作为高能底物和代谢调节物代偿ATP生成;增强组织细胞抗氧化能力,增加红细胞数量,及调节代谢紊乱;提高无氧、有氧运动能力,有利于疲劳的恢复。  相似文献   
13.
目的:为医疗机构研究设计一种新型的厌氧运送盒,用于运送厌氧固体标本,使标本处于厌氧环境。方法用高度透明、密封性能强的材料制备密封盒。盒内放置临床标本,设置插管等容器,通过冷触媒法在密封盒体内发生化学反应,从而消耗氧气。结果根据临床情况,研制出不同体积的运送盒,放入不同体积的标本,加入不同用量的化学药品、催化剂和指示剂。这种密封盒体,通过化学反应达到厌氧环境。结论此新型厌氧运送盒设计新颖,制作程序简捷,密封效果好,药物反应可控,厌氧指示科学,使用方便。  相似文献   
14.
The aim of this study was to determine whether there is an effect of time of day on the adaptation to strength training at maximal effort. Fourteen participants took part in this experiment. Their peak anaerobic power (Wingate anaerobic test) and peak knee extension torque at six angular velocities (1.05, 2.10, 3.14, 4.19, 5.24 and 6.29 rad · s -1 ) were recorded in the morning (between 07:00 and 08:00 h) and in the evening (between 17:00 and 18:00 h) just before and 2 weeks after a 6 week course of regular training. Seven of them trained only in the morning and seven only in the evening. Multivariate analysis of variance revealed a significant group 2 pre-/post-training 2 time of day interaction effect for peak torque and peak anaerobic power. Before training, in both groups, peak torque and peak anaerobic power were significantly higher in the evening than in the morning. After training, there was no significant difference in peak torque and peak anaerobic power between the morning and the evening for the morning training group. In contrast, in the evening training group, peak torque and peak anaerobic power were higher in the evening than in the morning. As a result of training, both peak torque and peak anaerobic power increased from their initial values as expected. The morning training group improved their peak anaerobic power significantly in the morning and in the evening, the absolute increase being larger in the morning than in the evening. The evening training group did not improve their peak anaerobic power in the morning, whereas it improved significantly in the evening. Although peak torque was significantly improved by training in the morning and evening in both groups, the absolute increase was greater in the morning than in the evening in the morning training group, whereas the opposite was the case for the evening training group. These results suggest that training twice a week at a specific hour increases the peak torque and the peak anaerobic power specifically at this hour and demonstrates that there is a temporal specificity to strength training.  相似文献   
15.
The purpose of this study was to provide a more detailed analysis of performance in cross-country skiing by combining findings from a differential global positioning system (dGPS), metabolic gas measurements, speed in different sections of a ski-course and treadmill threshold data. Ten male skiers participated in a freestyle skiing field test (5.6?km), which was performed with dGPS and metabolic gas measurements. A treadmill running threshold test was also performed and the following parameters were derived: anaerobic threshold, threshold of decompensated metabolic acidosis, respiratory exchange ratio = 1, onset of blood lactate accumulation and peak oxygen uptake ([Vdot]O2peak). The combined dGPS and metabolic gas measurements made detailed analysis of performance possible. The strongest correlations between the treadmill data and final skiing field test time were for [Vdot]O2peak (l?·?min?1), respiratory exchange ratio = 1 (l?·?min?1) and onset of blood lactate accumulation (l?·?min?1) (r = ?0.644 to ??0.750). However, all treadmill test data displayed stronger associations with speed in different stretches of the course than with final time, which stresses the value of a detailed analysis of performance in cross-country skiing. Mean oxygen uptake ([Vdot]O2) in a particular stretch in relation to speed in the same stretch displayed its strongest correlation coefficients in most stretches when [Vdot]O2 was presented in units litres per minute, rather than when [Vdot]O2 was normalized to body mass (ml?·?kg?1?·?min?1 and ml?·?min?1?·?kg?2/3). This suggests that heavy cross-country skiers have an advantage over their lighter counterparts. In one steep uphill stretch, however, [Vdot]O2 (ml?·?min?1?·?kg?2/3) displayed the strongest association with speed, suggesting that in steep uphill sections light skiers could have an advantage over heavier skiers.  相似文献   
16.
The aim of this study was to determine the effects of external loading on power output during a squat jump on a force platform in athletes specializing in strength and power events (6 elite weight-lifters and 16 volleyball players) and in 20 sedentary individuals. Instantaneous power was computed from time-force curves during vertical jumps with and without an external load (0, 5 or 10 kg worn in a special vest). The jumps were performed from a squat position, without lower limb counter-movement or an arm swing. Peak instantaneous power corresponded to the highest value of instantaneous power during jumping. Average power throughout the push phase of the jump was also calculated. A two‐way analysis of variance showed significant interactions between the load and group effects for peak instantaneous power ( P < 0.01) and average power ( P < 0.001). Peak instantaneous power decreased significantly in sedentary individuals when moderate external loads were added. The peak instantaneous power at 0 kg was greater than that at 5 and 10 kg in the sedentary individuals. In contrast, peak instantaneous power was independent of load in the strength and power athletes. Mean power at 0 kg was significantly lower than at 5 kg in the athletes; at 0 kg it was significantly higher than at 10 kg in the sedentary males and at 5 and 10 kg in the sedentary females. In all groups, the force corresponding to peak instantaneous power increased and the velocity corresponding to peak instantaneous power decreased with external loading. The present results suggest that the effects of external loading on peak instantaneous power are not significant in strength and power athletes provided that the loads do not prevent peak velocity from being higher than the velocity that is optimal for maximal power output.  相似文献   
17.
Abstract

The aim of this study was to compare the ‘anaerobic threshold’ (AnT) of subjects determined during a continuous 2‐min incremental exercise test until exhaustion and the ‘maximal lactate steady‐state’ (BLaSsmax) determined during prolonged exercise at constant loads corresponding to the subjects’ AnT and/or 5–25% above and below it. Seventeen subjects performed an incremental exercise test and 1–5 prolonged exercise tests on a cycle ergometer until exhaustion at intervals of 1 week, and work rates, oxygen uptake (VO2) values and brachial venous blood lactate (BLa) levels were measured. It was proposed that when exercising at a constant workload below AnT, BLa would fall after having reached its peak; at the level of AnT, BLa reaches maximal steady‐state (BLaSsmax); and above AnT, BLa increases continuously. Altogether, in 34 of 45 tests with a constant workload between 80 and 125% AnT, BLa values were as expected. In those cases in which BLaSsmax was reached, BLa increased on average by 3.8 mM from resting levels. This increase was 2.0 mM greater than that seen between resting levels and AnT during incremental exercise. There was no correlation between BLa values at BLaSsmax and at AnT, both when expressed as an increase in BLa (ABLa) and absolute BLa concentration. Altogether, 81% of the variation in BLa concentration at BLaSsmax could be explained by the subjects’ age, the percentage of slow‐twitch fibres and BLa levels at rest. The AnT and BLaSsmax did not differ significantly, and these values were correlated (r = 0.83). Together, AnT and age accounted for 85% of the variation seen in BLaSsmax. The BLaSsmax did not correlate with AnT when fixed at a BLa concentration of 4 mM (AnT4mM). The three hypotheses tested in this study were confirmed, and the present results demonstrate that AnT correlates with BLaSsmax. The few exceptions to anticipated BLa kinetics were small in magnitude and could be explained by physiological variations.  相似文献   
18.
厌氧颗粒污泥同时反硝化与产甲烷的研究   总被引:1,自引:0,他引:1  
文章通过向严格厌氧系统中投加硝酸盐后,系统对基质的降解情况来考察厌氧颗粒污泥的同时反硝化与产甲烷的可行性。试验结果表明,向厌氧颗粒污泥系统中投加硝酸盐后,反硝化优先进行,当反硝化完成后,产甲烷过程开始。在反硝化过程中,硝酸盐迅速被还原,有亚硝酸盐累积现象出现。向纯厌氧颗粒污泥系统中投加少量的好氧活性污泥后,反硝化速度加快,亚硝酸盐累积强度减弱。  相似文献   
19.
ABR/氧化沟/膜生物反应器处理垃圾渗滤液   总被引:1,自引:0,他引:1  
针对桂林市冲口垃圾卫生填埋场垃圾渗滤液出水未能达标排放的实际情况,根据连续多月监测垃圾渗滤液水质状况,选择折流式厌氧反应器(ABR)/氧化沟/膜生物反应器组合工艺进行处理.该工艺建成后实际运行效果良好,多年运行表明系统耐负荷能力极强,厂区周围的地表水质得到了极大改善.文中介绍了该渗滤液处理系统的组成、重要构筑物及其设计参数等情况,总结了运行效果和工艺特点.  相似文献   
20.
文章主要对高校优秀短跑运动员进行不同距离的速度练习后的血乳酸进行研究,经测试结果发现,30m-40m重复跑每次跑后的血乳酸无显著性差异,45m-60m距离跑后血乳酸急剧升高,形成血乳酸激增的转折阶段,60m与80m重复跑每次跑后的血乳酸值亦无显著差异,但60m和80m跑段的血乳酸值与30m-45m相比,差异非常显著。在此30m-45m距离是发展磷酸原系统供能能力的最适跑距,之后的60-80m主要发展速度耐力。  相似文献   
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