运动对心肌、骨骼肌HSP70表达影响的研究进展     

曹桂霞  白俊伟 《吉林体育学院学报》2010,26(5):83-85

热休克蛋白的分子伴侣、抗氧化、抗损伤、协同免疫及抗细胞凋亡等多种功能,不仅生物界对它越来越关注,而且运动医学界的学者也对它产生了浓厚的兴趣。HSP70作为细胞应激蛋白,对不同强度与形式的运动,其反应和适应有很大差异,这为我们进一步认识运动规律,从而进行科学的体育锻炼和运动训练提供了新的思路和方法。  相似文献   

热休克蛋白70及运动对衰老的影响研究     

石真玉  别业军  吴剑明  王薇  何亚新 《军事体育进修学院学报》2013,(2):122-124

衰老的机制到目前为止没有确切的理论解释,许多研究表明衰老与HSP70的关系密切.论文对HSP70与衰老之间的相互影响及其机制等方面进行了综述,并着重探讨了运动诱导的HSP70对衰老机体的作用,以期为运动延缓衰老提供有力的理论依据.  相似文献   

Effects of angiotensin Ⅱ on connexin 43 of VSMCs in arteriosclerosis     

CAI Wei RUAN Li-ming WANG Yi-na CHEN Jun-zhu 《Journal of Zhejiang University. Science. B》2006,7(8):648-653

Objective： To observe the effect ofangiotensin Ⅱ （Ang Ⅱ） on expression of gap junction channel protein connexin 43 （Cx43） in the proliferation process of vascular smooth muscle cells （VSMCs） during the early stage of arteriosclerosis. Methods： Thirty-two adult male rabbits were randomly divided into 4 groups. Rabbits in Group A were fed common diet while others in Groups B, C, and D were fed high-cholesterol diet. Losartan （10 mg/（kg.d）） and ramipril （0.5 mg/（kg-d）） were added in the diet of Groups C and D, respectively. The animals were sacrificed after 8 weeks and abdominal aortas were removed and dissected. The expression of Cx43 was assayed using RT-PCR and Western Blotting analysis. Results： Cx43 was increased markedly in both protein and mRNA level in Groups B, C, and D fed high-cholesterol diet compared with that in control group （P〈0.01）. Cx43 level in losartan or ramipril treated groups was higher than that in control group （P〈0.01, P〈0.05）, but lower than that in high-cholesterol diet groups （P〈0.05, P〈0.01）. Conclusion： Cx43 level was upregulated in VSMCs during early atherosclerosis. Losartan and ramipril can inhibit the expression of Cx43.  相似文献   

Sequence- and structure-selective mRNA m5C methylation by NSUN6 in animals     

Jianheng Liu  Tao Huang  Yusen Zhang  Tianxuan Zhao  Xueni Zhao  Wanying Chen  Rui Zhang 《国家科学评论(英文版)》2021,8(6)

mRNA m⁵C, which has recently been implicated in the regulation of mRNA mobility, metabolism and translation, plays important regulatory roles in various biological events. Two types of m⁵C sites are found in mRNAs. Type I m⁵C sites, which contain a downstream G-rich triplet motif and are computationally predicted to be located at the 5^′ end of putative hairpin structures, are methylated by NSUN2. Type II m⁵C sites contain a downstream UCCA motif and are computationally predicted to be located in the loops of putative hairpin structures. However, their biogenesis remains unknown. Here we identified NSUN6, a methyltransferase that is known to methylate C72 of tRNA^Thr and tRNA^Cys, as an mRNA methyltransferase that targets Type II m⁵C sites. Combining the RNA secondary structure prediction, miCLIP, and results from a high-throughput mutagenesis analysis, we determined the RNA sequence and structural features governing the specificity of NSUN6-mediated mRNA methylation. Integrating these features into an NSUN6-RNA structural model, we identified an NSUN6 variant that largely loses tRNA methylation but retains mRNA methylation ability. Finally, we revealed a weak negative correlation between m⁵C methylation and translation efficiency. Our findings uncover that mRNA m⁵C is tightly controlled by an elaborate two-enzyme system, and the protein-RNA structure analysis strategy established may be applied to other RNA modification writers to distinguish the functions of different RNA substrates of a writer protein.  相似文献   

"后基因组时代"的营养学研究     

常丽君  王燕  张治洲 《内蒙古科技与经济》2007,(17):35-36

飞速发展的基因科技,已将我们带入了"后基因组时代",伴随着基因组学、转录组学的迅猛发展,营养学的研究转向了研究营养与基因的相互作用,即营养基因组学.我们每个人自身的基因不同,某种特定的食物不见得对每个人有相同的"好处"或"坏处".要决定健康,关键要看基因和营养二者的相互作用.通过单核苷酸多态性,以及可以快速检测出mRNA水平的高通量基因表达谱,研究者可以更全面,更深入地了解营养与基因表达的关系,帮助指导人们根据自己的"基因要求",合理的搭配营养,实现营养个体化.  相似文献   

热休克蛋白(HSP70、HSP27)在食管癌组织中表达的意义     

张世棋  马春梅  蒋丽娜  薄爱华  张晓丽  邢立强 《河北北方学院学报(医学版)》2002,19(2):56-57

目的 :探讨HSP70和HSP2 7在食管癌组织中的表达情况。方法 :采用SP免疫组织化学染色检测 5 9例食管癌组织中HSP70和HSP2 7。结果 :HSP70和HSP2 7的总阳性率分别为 6 4.4 1% (38/5 9)和 5 8.6 2 % (34/5 8) ,HSP70和HSP2 7的共同阳性率为 30 .0 0 % (9/30 )。结论 :在食管癌中HSP70和HSP2 7普遍表达。  相似文献   

热休克蛋白65与血管内皮生长因子121偶联复合物的免疫学特性研究     

周红霞  刘景晶 《南京晓庄学院学报》2008,(6)

为了提高血管内皮生长因子(VEGF)121的免疫原型,文章用戊二醛一步偶联法将VEGF121与HSP65偶联形成HSP65-VEGF121复合物,并研究其免疫学效果,结果发现HSP65可以提高VEGF121的免疫原性,但抗肿瘤效果并未显著增强.  相似文献   

“70后”的成年礼——论《少年巴比伦》的启蒙叙事   总被引：1，自引：0，他引：1  

申霞艳 《海南师范大学学报(社会科学版)》2010,23(2):45-48

论文将路内的长篇小说《少年巴比伦》放置于世界文学成长小说的语境中进行解读,对身体与启蒙的关系进行了深入的探讨,并试图以此为例对＂70后＂的审美特征及局限进行分析。  相似文献   

Education and selective vouchers     

Amedeo Piolatto 《Economics of Education Review》2010

A widely accepted result in the literature is that the majority of voters are against the introduction of universal vouchers. Chen and West (2000) predict that voters’ attitudes towards selective vouchers (SV) may be different. They claim that voters are indifferent between the no-voucher and SV regimes, unless competition leads to a reduction in the education price. I show that, when public schools are congested, the majority of voters are in favour of SV. Furthermore, SV induces a Pareto improvement. In equilibrium, the introduction of SV induces a reduction in income stratification at school, with some relatively poor students attending private schools.  相似文献   

不同运动强度对大鼠肾小管钠离子依赖性枸橼酸转运蛋白基因表达作用的研究     

吴丽霞  曹建民  郭林  李海河  高连峰 《北京体育大学学报》2004,27(3):339-340

观察分析在不同的运动强度诱导的"运动性酸中毒"环境中,大鼠肾小管上皮细胞刷状缘侧膜钠离子依赖性枸橼酸转运蛋白mRNA表达量的变化.结果表明:不同运动强度对枸橼酸转蛋白mRNA表达量的作用不一.A组运动强度较小,血乳酸的水平相对于对照组无显著性升高(P>0.05),转运蛋白mRNA表达量也无显著性差异(P>0.05).B组和C组运动强度较大,血乳酸水平相对于对照组显著性升高(P>0.05),转运蛋白mRNA表达量也呈现明显增加(P<0.05).C组较B组血乳酸水平显著性升高(P<0.05),但两组转运蛋白mRNA表达量却无显著性差异(P>0.05),提示可能存在酸碱度变化对转运蛋白mRNA表达量影响作用的阈值.  相似文献