山莨菪碱清除氧自由基和抗脂质过氧化作用     

刘磊  张学梅  范劲松 《大连大学学报》1995,(3)

山莨菪碱对Fenton反应生成的·OH具育较强的直接清除作用,其IC_(50)为276mg.L~(-1),作用比·OH特异性清除剂甘露醇(IC_(50)为1349mg·L~(-1)强。对黄嘌呤-黄嘌呤氧化酶系统产生的O(?)也有较强的清除作用,其IC_(50)126mg·L~(-1)。山莨菪碱对自由基发生系统(FRGS)诱导离体大鼠心肌匀浆组织产生的脂质过氧化(LPO)作用有明显的抑制效应。对FRGS诱导离体大鼠心肌线粒体膜LPO也有一定的抑制作用。结果提示山莨菪碱的抗脂质过氧化作用与清除氧自由基作用有关。  相似文献   

Influence of green tea polyphenols on mitochondrial permeability transition pore and Ca2+ transport     

Shen Sheng-rong  Yu Hai-ning  Jin Chao-fang 《浙江大学学报(A卷英文版)》2002,3(4):475-479

The authors investigated the influence of green tea polyphenols (GTPs) on the liver mitochondria permeability transition pore (PTP) opening through mitochondria swelling and change of mitochondria membrane potential. The data showed that GTPs had obvious protective effect on the Ca²⁺-induced PTP opening in a dose-dependent manner detected by mitochondria swelling. The results were obtained by measuring the change of mitochondria membrane potential through Rh 123. Further experiments were conducted to examine the detailed influence of GTPs on Ca²⁺ import and export of mitochondria. The results showed that GTPs had remarkably inhibitory effect on the Ca²⁺-induced Ca²⁺ import in mitochondria; and that they could accelerate Ca²⁺-release from mitochondria. Our data provide an alternate interpretation of the potent protective function of GTPs on cell against apoptosis.  相似文献   

Up-regulation of mitochondrial antioxidation signals in ovarian cancer cells with aggressive biologic behavior     

Wang Y  Dong L  Cui H  Shen DH  Wang Y  Chang XH  Fu TY  Ye X  Yao YY 《Journal of Zhejiang University. Science. B》2011,12(5):346-356

Objective  

Recently, a high frequency of mutations in mitochondrial DNA (mtDNA) has been detected in ovarian cancer. To explore the alterations of proteins in mitochondria in ovarian cancer, a pair of human ovarian carcinoma cell lines (SKOV3/SKOV3.ip1) with different metastatic potentials was examined.  相似文献   

耐力运动与高脂饮食对Wistar大鼠骨骼肌线粒体融合分裂蛋白表达的影响研究     

郑义  徐晓阳  薄海  张勇 《广州体育学院学报》2021,(1)

目的:观察耐力运动与高脂饮食干预对肥胖大鼠骨骼肌线粒体融合与分裂蛋白表达的影响。方法:48只Wistar大鼠分N组(16只)与H组(32只)普食/高脂饲喂造模8周,随后分为NNC组、NNE组、HHC组、HHE组、HNC组与HNE组,NNC组、NNE组、HNC组与HNE组使用普食饲喂9周,HHC组与HHE组继续使用高脂饲喂9周,NNE组、HHE组与HNE组进行9周耐力运动干预。结果:高脂饲喂后大鼠骨骼肌线粒体融合蛋白MFN1、MFN2表达减少,分裂蛋白FIS1表达增加,耐力运动干预增加了高脂饲喂后大鼠骨骼肌线粒体MFN1、MFN2的表达,降低了DRP1、FIS1的表达。结论:高脂饲喂显著降低了骨骼肌线粒体融合蛋白的表达,增加了分裂蛋白的表达,耐力运动干预显著增加了骨骼肌线粒体融合蛋白的表达,显著降低了HFD干预后骨骼肌线粒体分裂蛋白的表达。  相似文献