仙人掌科植物组织培养研究进展   总被引：4，自引：0，他引：4  

张乍如  闾秋敏 《岳阳职业技术学院学报》2006,21(2):56-58

仙人掌科植物是一类日益受到重视的观赏、食用、药用植物。其组织培养研究在组培对象、外植体及其消毒、培养基、成苗途径等方面已取得了巨大进展,并对与植物学有关的研究领域产生了较大影响。  相似文献   

Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2     

Jiang Qiang  Zhang Su-zhan  Peng Jia-ping  Wang Xu-lin 《浙江大学学报(A卷英文版)》2005,6(9):859-864

Objective: To prepare microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2 (TIMP-2), and investigate their biological characteristics in vitro. Methods: Chinese hamster ovary (CHO) cells were stably transfected with a human TIMP-2 expression vector, encapsulated in barium alginate microcapsules and cultured in vitro. Morphological appearance of the microcapsules was observed under a light microscope. Cell viability was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Enzyme linked immunosorbent assay (ELISA) and reverse zymography were used to confirm the release of biologically active TIMP-2 from the microcapsules. Cryopreservation study of the microencapsulated cells was carried out using dimethyl sulfoxide (DMSO) as preservative agent. Results: The microcapsules appeared like a sphere with diameter of 300–600 μm. The surface of the capsule wall was clearly smooth. The microencapsulated cells survived well and kept proliferating over the 6 weeks observed. No significant difference in TIMP-2 secretion was found between encapsulated and unencapsulated cells. Reverse zymography confirmed the bioactivity of MMP (matrix metalloproteinase) inhibition of TIMP-2. The cryopreservation process did not damage the microcapsule morphology nor the viability of the cells inside. Conclusion: Microencapsulated engineered CHO cells survive at least 6 weeks after preparation in vitro, and secrete bioactive TIMP-2 freely from the microcapsules.  相似文献   

黄鼬呼吸器官的组织学研究     

朱茂英  王珏  王政富  金光明 《安徽科技学院学报》1994,(1)

本项研究结果表明;黄鼬呼吸器官的组织结构与其他哺乳动物呼吸器官的组织结构相比有其重要特征.(1)气管粘膜上皮内无杯状细胞;(2)粘膜下层内气管腺特别发达;(3)气管的外膜由1一4层弧形的透明软骨间断排列构成支架,软骨外侧平滑肌特别发达呈环行的带状;(4)肺内各级支气管壁平滑肌都较发达,粘膜皱襞明显;(5)肺泡隔厚,其结缔组织中的细胞成份多,排列紧密.  相似文献   

不同激素配比对铁皮石斛组织培养的影响研究   总被引：13，自引：0，他引：13       下载免费PDF全文

陈兆贵  谭俊 《惠州学院学报》2006,26(3):11-14

在铁皮石斛组织培养过程中,通过筛选适宜铁皮石斛原球茎增殖、分化、壮苗和生根的培养基,以提高增殖系数和试管苗的质量。结果表明:原球茎增殖以MS NAA1．0 mg／L 6-BA1．0 mg／L KT1．0 mg／L为最佳;原球茎分化以MS NAA1．0 mg／L 6-BA3．0mg／L KT1．0 mg／L为最佳;壮苗生根以1／2MS IBA2．0 mg／L 水解酪蛋白500mg／L 10％香蕉提取液为最佳,45天生根率达92％。  相似文献   

中药王不留行的组织培养技术初探   总被引：1，自引：0，他引：1  

贲爱玲  蔡小宁  任源浩  汪晓婷 《南京晓庄学院学报》2006,22(6):53-56

选取王不留行饱满种子培养无菌苗,用无菌苗的茎和叶作为外植体,分别接种MS基本培养基外加激素NAA和6-BA诱导愈伤组织和不定芽,使用1/2MS培养基,附加IBA或NAA进行不定芽的生根实验,最后炼苗和移栽.实验结果表明王不留行的最优组织培养方案:种子用0.1%升汞消毒8m in,无菌湿脱脂棉催芽,茎和叶作为外植体,使用MS 0.1mg/L NAA 0.1mg/L 6-BA诱导愈伤组织和不定芽,1/2MS 0.15mg/L NAA诱导植株生根.  相似文献   

生物组织光学特性参数测量原理及设计   总被引：1，自引：0，他引：1  

张连顺 《德州学院学报》2001,17(4):16-20

从光的传输理论出发，利用漫射近似方程，推导出当无限细脉冲光及连续光束垂直入射到生物组织表面时，生物组织表面漫反射光随空间及时间变化的解析表达式，由此表达式出发，设计了一套实验装置，分析了生物组织光学特性参数的测量方法，以及如何对实验数据进行处理。  相似文献   

果树组织培养脱毒技术和病毒检测技术研究进展   总被引：2，自引：0，他引：2  

李俊芳  侯义龙  苏福才  张立娟  于亚军 《大连大学学报》2006,27(2):10-15

病毒病对果树的危害日趋严重,各国对病毒病害的研究和防治极为重视.作者着重论述当前果树生产上主要应用的果树组织培养技术、脱除病毒技术和病毒检测技术,包括其原理、操作过程和特点,并简要探讨了病毒检测与脱毒的关系.  相似文献   

MEASUREMENT OF THE DIELECTRIC PROPERTIES OF BIOLOGICAL TISSUE     

顾本立  黄雷 《东南大学学报》1989,(1)

An open-ended coaxial line reflection method especially suitable for meas-uring the dielectric properties of biological tissue in vivo is described.This method offersthe advantage of not requiring any special preparation of the samples to be measured but aclose contact with the open end of a coaxial line.It is,therefore,very convenient to acquirea large number of measurement data in broad band rapidly.The method may also be usedto measure the properties of other substances.The measuring system consists of a networkanalyzer controlled by a microcomputer and calibrated by using ANA procedure to elimi-hate the influnce of error network introduced by the adapter,some connectors,etc.In or-der to reach higher accuracy,the iterative method is used to determine the parameters ofthe equivalent circuit.Measurements of permeativities of some living tissues have been per-formed in the frequency band of 0.5-2GHz.Compared with the results known in somepapers,the validity of this method has been confirmed.The difference in dielectric proper-ties between living and dead tissues,and the tissue permeativites(ε)versus frequency andduration of measurement after death have also been measured.  相似文献   

祁术的组织培养及快速繁殖技术研究     

胡长玉  叶玉娟  郝祥霞  胡跃 《黄山学院学报》2006,8(5):57-60

为研究祁术的组织培养及快速繁殖技术,选用祁术的叶片和下胚轴为外植体材料,接种于附加植物生长调节剂NAA,6-BA及其组合的MS固体培养基上.结果表明:下胚轴为外植体明显好于叶片,对于愈伤组织形成,叶片采用MS NAA(0.5mg/L) 6-BA(2mg/L);下胚轴MS NAA(0.5mg/L) 6-BA(2mg/L);MS NAA(0.5mg/L) 6-BA(1mg/L)为宜.对于叶片丛生芽愈伤组织培养基用MS NAA(0.2mg/L) 6-BA(2.8mg/L),下胚轴丛生芽愈伤组织培养基用MS NAA(0.01mg/L) 6-BA(0.1mg/L);MS NAA(0.05mg/L) 6-BA(0.1mg/L)为宜,诱导生根以1/2MS NAA0.3mg/L最佳,生根率达95%以上.  相似文献   

优质葡萄品种组培快繁育苗工厂化技术研究   总被引：1，自引：0，他引：1  

何家涛 《襄樊职业技术学院学报》2002,1(1):16-19

本实验研究了探讨了葡萄组培中的外植体诱导建立无菌繁殖系；外植体生长分化和增殖培养基的筛选；壮苗生根及驯化移栽等关键技术环节，从而使优质葡萄品种组培快繁工厂化大规模育苗成为可能。  相似文献