几个重要生物实验的改进     

杨特清 《中国科教创新导刊》2011,(16):86-86

本文通过实践摸索,发现几个重要生物实验中存在的弊端,并探索出一些行之有效,简单易行的改进方法,给同行提供参考。  相似文献   

Determination of Biological Variance and Validation of a Fluorometric Assay for Measurement of α-l-Iduronidase Activity in Dried Blood Spots Samples: The First Experience in Iran     

Mohammad Abdi  Mohammad Said Hakhamaneshi  Mohammad Reza Alaei  Namam-Ali Azadi  Rahim Vakili  Daniel Zamanfar  Mohammad Taghikhani  Shohreh Khatami 《Indian journal of clinical biochemistry : IJCB》2015,30(3):318-322

Methods for assaying lysosomal diseases in dried blood samples are very useful today due to its several advantages related to the stability of samples, its transportation, handled and analysis, and its potential use for newborn screening compared to traditional methods in leucocytes samples. For this reason, it is important to validate these assays before being used in routine laboratory. Because of different in biological markers based on ethnicity, we aimed this study to validation a DBS-based fluorometric assay for measurement of α-l-Iduronidase activity for diagnosis of MPS I patients in Iran. DBS samples were collected from 15 MPS I patients and 60 healthy age matched subjects. Diagnostic value, biological variance and α-l-Iduronidase activity were determined. DBS α-l-Iduronidase activity was significantly higher in male subjects than in female group. Using a cut-off level of 1.08 µmol/spot 20 h, sensitivity and specificity were 100 and 98 %. The linearity of test was proved and we showed that within-run and between run precision were 5.6 and 14.66 %. Measurement of α-l-Iduronidase activity in DBS samples is an accurate test for diagnosis of MPS I and because of its rapid shipping and simplicity to keeping, DBS-based enzyme activity could be considered as a useful diagnostic tool in this disease.  相似文献   

Association of Aberrations in One Carbon Metabolism with Intimal Medial Thickening in Patients with Type 2 Diabetes Mellitus     

R. Dhananjayan  T. Malati  Y. Rupasree  Vijay Kumar Kutala 《Indian journal of clinical biochemistry : IJCB》2015,30(3):263-270

The present work was aimed to study the association of one carbon genetic variants, hyperhomocysteinemia and oxidative stress markers, i.e., serum nitrite, plasma malondialdehyde (MDA) and glutathione (GSH) on intimal medial thickening (IMT) in patients with type 2 diabetes mellitus (T2D). A total number of 76 subjects from ACS Medical College and Hospital, Chennai, India were included in the study, i.e., Group I (n = 42) of T2D and Group II (n = 34) of age- and sex matched healthy controls. The glycated haemoglobin was measured by ion-exchange resin method; plasma homocysteine by Enzyme Linked Immunosorbant Assay method; serum nitrite (nitric oxide, NO), plasma MDA and GSH by spectrophotometric methods; the IMT by high frequency ultrasound. The polymorphisms of one carbon genetic variants were genotyped using polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism methods. Results indicate that methyltetrahydrofolate homocysteine methyl transferase (MTR) A2756G allele was found to be protective in T2D and the other variants were not significantly associated with T2D. Glutamate carboxypeptidase II (GCP II) C1561T (r = 0.34; p = 0.05) and methylene tetrahydrofolate reductase (MTHFR) C677T (r = 0.35; 0.04) showed positive correlation with plasma homocysteine in T2D cases. In this study, MTR A2756G allele was found to be protective in T2D; GCP II C1561T and MTHFR C677T showed positive association with plasma homocysteine in T2D cases. Among all the genetic variants, MTR A2756G was found influence IMT. RFC 1 G80A and TYMS 5′-UTR 2R3R showed synergistically interact with MTR A2756G in influencing increase in IMT.  相似文献   

女子赛艇八单专项训练强度测试分析与运用     

孙朋  张国培 《湖北体育科技》2015,(6):516-518

通过监控女子赛艇八单赛前40日训练,对安静状态和不同桨频训练下血乳酸分析,寻找变化规律,找出不同桨频的适宜训练强度,为不同训练负荷提供理论依据。  相似文献   

顺铂联合热处理对术中回收血红细胞及其中混杂的肝肿瘤细胞株(HepG2)的影响（英文）     

Jin-ting YANG  Li-hui TANG  Yun-qing LIU  Yin WANG  Lie-ju WANG  Feng-jiang ZHANG  Min YAN 《Journal of Zhejiang University. Science. B》2015,16(5):395-403

目的:研究离体热处理和顺铂联合热处理对术中回收血红细胞功能的影响及其中混杂的肝肿瘤细胞株(HepG2)的杀灭作用。创新点:(1)采用多种评价指标研究了不同时间离体热处理对术中回收血中混杂的HepG2的杀灭作用及对红细胞的影响,并确定了对红细胞安全且能有效杀灭HepG2的离体热处理时间。(2)从多个角度评价了离体顺铂联合热处理对术中回收血红细胞的影响及对其中混杂的HepG2的杀灭作用,确定了该方案中对红细胞安全且能有效去除HepG2的顺铂浓度。方法:采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)、5-乙炔基-2’脱氧尿嘧啶核苷(EdU)和平板克隆形成评估HepG2的细胞存活率、DNA复制率和克隆形成能力(图1和3);从红细胞渗透脆性、携氧能力(2,3-二磷酸甘油酸(2,3-DPG)、半饱和氧分压(P50))、能量代谢(Na+-K+-ATPase、pH)、膜完整性(游离血红蛋白(Hb)、血清K+和Na+浓度、细胞膜磷脂酰丝氨酸外翻比例)等角度评估红细胞功能(图2和4;表3)。结论:肝肿瘤术中回收血经离体顺铂联合热处理(42°C,50μg/ml)60min后,能有效清除其中混杂的HepG2,但对红细胞无显著影响,值得体内进一步研究顺铂热处理有效应用于肿瘤手术自体血液回输的安全方案。  相似文献   

SYSMEX UF-1000i尿有形成分分析仪对尿路感染的诊断价值     

战伟 《大连大学学报》2015,(3):100-102

采用Sysmex UF-1000i尿有形成分分析仪和AX-4280尿干化学分析仪测定尿标本,同时进行尿细菌定量培养。把测定值与尿培养金标准比对,干化学法白细胞酯酶和UF-1000i细菌计数结果的一致性适中,与亚硝酸盐结果的一致性较低,与尿白细胞计数结果的一致性极高。细菌定量培养的特异性、灵敏度及一致性高于UF-1000i白细胞计数。UF-1000i在临床快速诊断尿路感染时应用价值较高。  相似文献   

补充锌元素对高强度运动肾促红细胞生成影响     

郭官在 《科技通报》2014,(12)

目的:分析锌元素对高强度运动肾促红细胞生成的影响。方法:选取某体育学院60名男性运动员,将运动员随机分为对照组20人,运动组40人,进行长跑运动训练1周后,根据肾促红细胞血象参数、体重以及运动能力将运动组运动员,划分为运动组20人和补锌运动组20人,持续进行长跑运动和锌元素的补充,观察补充锌元素对高强度运动肾促红细胞血象以及血清肾促红细胞EPO水平的影响。结果:5周长跑运动训练后的运动组运动员与对照组肾促血细胞指标比较,运动组的Hb、RBC和Hct均显著低于对照组(P0.01)。对比补锌运动组和对照组可以看出,补锌运动组的肾促红细胞平均体积(MCV)明显高于对照组(P0.05),其肾促红细胞平均血红蛋白量(MCH)以及红细胞平均血红蛋白浓度(MCHC)明显低于对照组(P0.05),尤其两组的MCHC指标,具有非常显著性差异(P0.01),但两组间剩余肾促红细胞相关参数并无显著性差异(P0.05),补锌运动组的Hb、RBC、Hct水平明显高于运动组(P0.01或P0.05)。补锌运动组运动员的血清肾促EPO水平明显高于对照组和运动组,存在显著性差异(P0.05)。结论:补充锌元素能够促进运动员肾促红细胞的生成,锌元素在一定程度上能够提高机体的运动能力。  相似文献   

补阳还五汤治疗中风后遗症的研究进展     

张明明  邱全 《大众科技》2014,(11):126-127

补阳还五汤为清代名医王清任所创,始见于《医林改错》,由黄芪、当归、川芎、地龙、赤芍、桃仁、红花组成,具有补气活血通络的功效。临床的治疗研究与实验证明,本方对于治疗中风后遗症效果良好,尤其以气虚血瘀型效果明显。现代研究表明,补阳还五汤具有抗血栓、抗氧化、抗动脉粥样硬化、和促进神经系统修复等多种作用。文章综述了近年来补阳还五汤对中风后遗症的临床治疗实验及相关研究进展。  相似文献   

History of the preanalytical phase: a personal view     

Walter G. Guder 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2014,24(1):25-30

In the 70ies of the last century, ther term “preanalytical phase” was introduced in the literature. This term describes all actions and aspects of the “brain to brain circle” of the medical laboratory diagnostic procedure happening before the analytical phase. The author describes his personal experiences in the early seventies and the following history of increasing awareness of this phase as the main cause of “laboratory errors”. This includes the definitions of influence and interference factors as well as the first publications in book, internet, CD-Rom and recent App form over the past 40 years. In addition, a short summary of previous developments as prerequesits of laboratory diagnostic actions is described from the middle age matula for urine collection to the blood collection tubes, anticoagulants and centrifuges. The short review gives a personal view on the possible causes of missing awareness of preanalytical causes of error and future aspects of new techniques in regulation of requests to introduction of quality assurance programs for preanalytical factors.  相似文献   

Abnormal gel flotation in a patient with apperant pneumonia diagnosis: a case report     

Fethullah Gerin  Dilber Coban Ramazan  Ozgur Baykan  Onder Sirikci  Goncagul Haklar 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2014,24(1):180-182

Introduction:

Serum blood collection tubes with separator gel are widely used by many laboratories for chemistry analyses. We describe a case of a primary blood collection tube filled with blood sample and a floating separator gel.

Materials and methods:

The blood sample was collected from a 51 years old female in intensive care unit with the diagnosis of pneumonia into a BD Vacutainer SST tube (Becton Dickinson, NJ, USA) containing serum separator gel and conveyed to the core laboratory of Marmara University Hospital within 30 minutes from collection. Sample was immediately centrifuged at room temperature at 1500 × g for 10 minutes.

Results:

The analyses revealed a highly increased total protein concentration of 145 g/L (reference interval 64–83 g/L). The nephelometric analyses showed an elevated serum IgG concentration of 108 g/L (reference interval 6.5–16 g/L) and IgG lambda monoclonal band was determined by serum immunofixation electrophoresis.

Conclusion:

Limitation of the separator gel tubes in patients with a high plasma density and its possible effects on test results and laboratory costs should be remembered. The clinical diagnosis stated in the information system should also reveal known comorbid conditions besides the apparent admission reason. This information would avoid resampling, additional testing, and communication efforts with the clinicians.  相似文献