生物大分子的共振光散射分析     

王峰 《忻州师范学院学报》2005,21(2):11-14

文章介绍了共振光散射技术及其在生物大分子定量分析中的应用,并讨论了其发展方向和前景。  相似文献   

Cyclooxygenase-2 inhibitor inhibits hippocampal synaptic reorganization in pilocarpine-induced status epilepticus rats     

Zhang HJ  Sun RP  Lei GF  Yang L  Liu CX 《Journal of Zhejiang University. Science. B》2008,9(11):903-915

  相似文献   

A New Potent Inhibitor of Thrombin from the Leech Haemendipsa Yanyuanensis   总被引：1，自引：0，他引：1  

ZHAO Rong le College of Life Sciences  Beijing Normal University  Beijing  China 《上海大学学报(英文版)》2001,(1)

1　Ｉｎｔｒｏｄｕｃｔｉｏｎ　Ｔｈｒｏｍｂｕｓｄｉｓｅａｓｅｏｆｈｅａｒｔａｎｄｂｒａｉｎｂｌｏｏｄｖｅｓｓｅｌｈａｓｂｅｃｏｍｅａｋｉｌｌｅｒｏｆｍａｎ .Ｂｅｃａｕｓｅｏｆｔｈｅｓｉｄｅｅｆｆｅｃｔｓｏｆｈａｅｍｏｒｒｈａｇｅｏｒｔｈｅｓｈｏｒｔｈａｌｆ ｌｉｆｅｉｎｖｉｖｏ ,ｔｈｅｐｒｅ ｓｅｎｔａｎｔｉｃｏａｇｕｌａｎｔｄｒｕｇｓａｒｅｕｎｓａｔｉｓｆｉｅｄ ,ａｎｄｓｅａｒｃｈ ｉｎｇｆｏｒｎｅｗａｎｔｉｃｏａｇｕｌａｎｔｓｗｉｔｈｈｉｇｈｅｒｅｆｆｉｃｉｅｎｔａｎｄｍｏｒｅｓｔａｂｉｌｅｈａ…  相似文献   

结构基因组学与蛋白质核磁共振(NMR)光谱     

李宏  姜怀春 《重庆第二师范学院学报》2006,19(3):25-27,31

阐述了核磁共振光谱在蛋白质结构基因组学研究中的重要作用,包括核磁共振光谱在结构基因组学研究中的优点和缺点,研究技术进展和展望。  相似文献   

抗冻蛋白的抗冻活性及其测定方法   总被引：1，自引：0，他引：1  

邵强  李海峰  刘国生  闫清华 《平原大学学报》2005,22(4):111-113

抗冻蛋白是一类能够保护生物体免受结冰引起伤害的蛋白质，其抗冻活性表现为热滞性和重结晶抑制效应。本文讨论了影响抗冻蛋白活性的因素，介绍了抗冻蛋白热滞活性和重结晶抑制效应的定量测定方法。  相似文献   

Troponin T and Heart Type Fatty Acid Binding Protein (h-Fabp) as Biomarkers in Patients Presenting with Chest Pain     

Lakshmi Lavanya Reddy  Swarup A. V. Shah  Alpa J. Dherai  Chandrashekhar K. Ponde  Tester F. Ashavaid 《Indian journal of clinical biochemistry : IJCB》2016,31(1):87-92

Acute coronary syndrome (ACS) is a term for a range of clinical signs and symptoms suggestive of myocardial ischemia. It results in functional and structural changes and ultimately releasing protein from injured cardiomyocytes. These cardiac markers play a major role in diagnosis and prognosis of ACS. This study aims to assess the efficacy of heart type fatty acid binding protein (h-FABP) as a marker for ACS along with the routinely used hs-TropT. In our observational study, plasma h-FABP (cut-off 6.32 ng/ml) and routinely done hs-Trop T (cutoff 0.1 and 0.014 ng/ml) were estimated by immunometric laboratory assays in 88 patients with acute chest pain. Based on the clinical and laboratory test findings the patients were grouped into ACS (n = 41) and non-ACS (n = 47). The diagnostic sensitivity, specificity, NPV, PPV and ROC curve at 95 % CI were determined. Sensitivity of hs-TropT (0.1 ng/ml), hs-TropT (0.014 ng/ml) and h-FABP were 53, 86 and 78 % respectively and specificity for the same were 98, 73 and 70 % respectively. Sensitivity, specificity and NPV calculated for a cut-off combination of hs-TropT 0.014 ng/ml and h-FABP was 100, 51 and 100 % respectively. These results were substantiated by ROC analysis. Measurement of plasma h-FABP and hs-TropT together on admission appears to be more precise predictor of ACS rather than either hs-Trop T or h-FABP.  相似文献   

Blood zinc protoporphyrin, serum total protein, and total cholesterol levels in automobile workshop workers in relation to lead toxicity: Our experience     

Suneesh Kumar Pachathundikandi  Earaly Thomas Varghese 《Indian journal of clinical biochemistry : IJCB》2006,21(2):114-117

Blood zinc protoporphyrin (ZPP), serum total protein (TP), and total cholesterol (TC) levels in automobile workshop workers in relation to lead toxicity were analysed. In the present study, automobile workshop workers (healthy male workers at an age between 28 and 35 from four major automobile workshops in Kottayam, Kerala State, India) and the control (male healthy adults at an age between 28 and 35 residing at Aymanam, a distant village at Kottayam District, Kerala having reduced or no chance of lead exposure) displayed significant difference in blood lead (BPb) and blood ZZP (BZPP) level. The mean value of BPb in automobile workshop workers was 15.76±0.33 μg/dl, while in the control it was 8.20±0.15 μg/dl. In automobile workshop workers, the mean value of BZPP was 34.2±0.62 μg/dl. The control group exhibited a mean of 11.5±0.22 μg/dl. Automobile workshop workers exhibited significant increase in BZPP was corresponding to the increase in BPb level. The total protein levels estimated in automobile workshop workers showed significant decrease compared to control individuals, but was within the reference range of healthy individuals. The mean value of TP level in automobile workshop workers and control was 6.9±0.13 g/dl and 7.71±0.18 g/dl, respectively. There was no significant difference in blood haemoglobin (BHb) level among the automobile workshop workers and control. The serum TC level in automobile workshop workers showed significant decrease compared to the control individuals, but was with in the reference range of healthy individuals. The mean level of serum TC in automobile workshop workers was 162.00±3.44 mg/dl and the same in control was 172.86±4.32 mg/dl. The present study affirms occupational lead toxicity in automobile workshop workers and its effect on serum protein and cholesterol levels.  相似文献   

Cell therapy in congestive heart failure   总被引：5，自引：0，他引：5  

Tao ZW  Li LG 《Journal of Zhejiang University. Science. B》2007,8(9):647-660

Congestive heart failure （CHF） has emerged as a major worldwide epidemic and its main causes seem to be the aging of the population and the survival of patients with post-myocardial infarction. Cardiomyocyte dropout （necrosis and apoptosis） plays a critical role in the progress of CHF; thus treatment of CHF by exogenous cell implantation will be a promising medical approach. In the acute phase of cardiac damage cardiac stem cells （CSCs） within the heart divide symmetrically and/or asymmetrically in response to the change of heart homeostasis, and at the same time homing of bone marrow stem cells （BMCs） to injured area is thought to occur, which not only reconstitutes CSC population to normal levels but also repairs the heart by differentiation into cardiac tissue. So far, basic studies by using potential sources such as BMCs and CSCs to treat animat CHF have shown improved ventricular remodelling and heart function. Recently, however, a few of randomized, double-blind, placebo-controlled clinical trials demonstrated mixed results in heart failure with BMC therapy during acute myocardial infarction.  相似文献   

Effect of a distal protection device on epicardial blood flow and myocardial perfusion in primary percutaneous coronary intervention   总被引：2，自引：0，他引：2  

Zhou BQ  Tahk SJ 《Journal of Zhejiang University. Science. B》2007,8(8):575-579

Objective: The beneficial effect of percutaneous coronary intervention (PCI) in patients with acute myocardial infarction (AMI) has been well established, but there is the problem of no-reflow phenomenon which is an adverse prognostic factor in primary PCI. In the present study the effect of a distal protection device (PercuSurge GuardWire; GW) on epicardial blood flow and myocardial perfusion was evaluated. Methods and Results: Patients with AMI were randomly divided into 2 groups, the GW and the control groups. The GW group included 52 patients with AMI who underwent primary PCI with GW protection and the control group included 60 patients who underwent primary PCI without GW protection. Epicardial blood flow in the infarct-related artery (IRA) and myocardial perfusion were evaluated according to the thrombolysis in myocardial infarction (TIMI) flow grade and the myocardial blush grade (MBG). We found TIMI score of 3 was obtained significantly more frequently in the GW group (96%) than in the control group (80%). The MBG score of 3 was obtained also significantly greater in the GW group (65%) than in the control group (33%). Conclusion: Primary PCI with GW protection can significantly improve epicardial blood flow and myocardial perfusion.  相似文献   

QTL analysis for some quantitative traits in bread wheat     

Pushpendra KG  Harindra SB  Pawan LK  Neeraj K  Ajay K  Reyazul RM  Amita M  Jitendra K 《Journal of Zhejiang University. Science. B》2007,8(11):807-814

Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain protein content (GPC) sug- gested that the major part of genetic variation for this trait is due to environmental interactions. In contrast, pre-harvest sprouting tolerance (PHST) was controlled mainly by main effect QTL (M-QTL) with very little genetic variation due to environmental interactions; a major QTL for PHST was detected on chromosome arm 3AL. For grain weight, one QTL each was detected on chromosome arms 1AS, 2BS and 7AS. QTL for 4 growth related traits taken together detected by different methods ranged from 37 to 40; nine QTL that were detected by single-locus as well as two-locus analyses were all M-QTL. Similarly, single-locus and two-locus QTL analyses for seven yield and yield contributing traits in two populations respectively allowed detection of 25 and 50 QTL by composite interval mapping (CIM), 16 and 25 QTL by multiple-trait composite interval mapping (MCIM) and 38 and 37 QTL by two-locus analyses. These studies should prove useful in QTL cloning and wheat improvement through marker aided selection.  相似文献