Comparison of Haptic and Biometric Properties,Bacterial Load,and Student Perception of Fixative Solutions: Formaldehyde Versus Chilean Conservative Fixative Solution with and without Formaldehyde in Pig Kidneys     

Marianne Skopnik-Chicago  Katherine Poblete-Cordero  Natali Zamora  Roberto Bastías  Pablo A. Lizana 《Anatomical sciences education》2021,14(6):836-846

One of the most widely used solutions to fix and preserve organic tissues is formaldehyde, despite reservations regarding its toxicity and the fact that formaldehyde-embalmed bodies lose their original characteristics. Anatomy laboratories have been replacing formaldehyde with solutions that retain the characteristics of fresh tissue. For this purpose, alternative solutions with a very low concentration of formaldehyde or without any formaldehyde have been analyzed. The objective of this study was to compare biometry, coloration, haptic properties, and bacterial load on animal specimens (pig kidneys) embalmed with formaldehyde, and with Chilean Conservative Fixative Solution with and without formaldehyde (formaldehyde chCFS and formaldehyde-free chCFS). Also, the perception of health and biological science students toward specimens treated with different solutions was assessed. The results indicated that there were no significant differences in specimens’ retraction, or bacterial load. Students showed a preference for organs embalmed in formaldehyde chCFS and formaldehyde-free chCFS; indicating that with these treatments they could better visualize structures and that the prosections had greater flexibility and the colors were more similar to those of fresh tissue. Additionally, students recommended the material embalmed in formaldehyde chCFS and formaldehyde-free chCFS for anatomy learning. In contrast, students indicated that formaldehyde-fixation negatively affected their practical experience. In conclusion, embalming with formaldehyde chCFS or formaldehyde-free chCFS provides an advantageous practical experience over the use of formaldehyde and may be an alternative to replace the use of formaldehyde in anatomy laboratories.  相似文献   

Effects of periodic carbohydrate ingestion on endurance and cognitive performances during a 40-km cycling time-trial under normobaric hypoxia in well-trained triathletes     

Yi-Hung Liao  Toby Mündel  Yan-Ting Yang  Chen-Chan Wei  Shiow-Chwen Tsai 《Journal of sports sciences》2019,37(16):1805-1815

The purpose of this study was to examine CHO ingestion on a cognitive task using a field-simulated time-trial (TT) under hypoxia in well-trained triathletes. Ten male triathletes (age: 22.1 ± 1.1 years; VO₂max: 59.4 ± 1.4 ml/kg/min) participated in this double-blind/crossover/counter-balanced design study. Participants completed 3 TT trials: 1) normoxic placebo (NPLA; FiO₂ = 20.9%), 2) hypoxic placebo (HPLA; FiO₂ = 16.3%), and 3) hypoxic CHO (HCHO; 6% CHO provided as 2 ml/kg/15 min; FiO₂ = 16.3%). During the TT, physiological responses (SpO₂, HR, RPE, and blood glucose/lactate), cognitive performance, and cerebral haemodynamics were measured. Hypoxia reduced TT performance by ~3.5–4% (p < 0.05), but CHO did not affect TT performance under hypoxia. For the cognitive task, CHO slightly preserved exercise-induced cognitive reaction speed but did not affect response accuracy during hypoxic exercise. However, CHO did not preserve the decreased Hb-Diff (cerebral blood flow, CBF) and increased HHb in the prefrontal lobe (p < 0.05) during hypoxic exercise, and CHO failed to preserve hypoxia-suppressed prefrontal CBF and tissue oxygen saturation. In conclusion, the present study demonstrates that CHO is effective in sustaining reaction speed for a cognitive task but not promoting TT performance during hypoxic exercise, which would be important for strategy-/decision-making when athletes compete at moderate high-altitude.  相似文献   

浅谈组织胚胎学教学方法     

马兴芬 《保山师专学报》2005,24(2):23-24

组织胚胎学是医学形态学的基础,也是学生进入医学领域的基础,要使学生打牢基础,必须抓住教学重点和难点,充分运用现代教学手段和现代教学条件,提高学生的求知欲望.  相似文献   

成龄栓皮栎组培苗生根影响因素研究   总被引：3，自引：0，他引：3  

杨锋利  杜保国  张存旭 《绵阳师范学院学报》2006,25(5):79-82,95

以成龄(30年以上)栓皮栎枝条的萌条在室内沙培催芽,用萌芽作为外植体建立无菌苗体系,研究了基本培养基、蔗糖浓度以及IBA浓度和处理时间对生根的影响。结果表明:采用1/4MS培养基添加30g/L蔗糖为较适宜的培养基,生根率达到了66.4%,平均生根数为2.3条,平均根长为4.82 cm。采用两步生根法,用添加1.0mg/L的IBA培养基处理14天,然后转接到无激素的培养基上的生根率最高,达到了96.7%,愈伤组织少。  相似文献   

高分子生物材料的组织工程支架成形方法     

岳秀艳  索海瑞  史廷春  彭东亮 《唐山学院学报》2008,21(2):55-60

多孔支架是组织工程的重要要素,高分子材料是制作支架的主要材料。而快速成形技术以其复杂形状成形能力、可控的连通孔造孔能力成为组织工程支架制备的一种崭新方法。讨论了组织工程对支架性能的要求,介绍了常用高分子材料作为支架主要特性以及相关的成形方法。通过与传统支架制备方法的对比,阐述了快速成形技术的优势。  相似文献   

大花蕙兰快速繁殖技术的研究     

鞠守勇  高小蛾  陈其国  李莉  高广斌 《武汉职业技术学院学报》2008,7(5):59-61

本文对大花蕙兰外植体消毒方式,原球茎增殖的培养基,大花蕙兰无根茵诱导生根培养基三个方面进行了系统的研究。结果表明：外植体在用A．1％Tween-60的1％HgCl2溶液消毒8分钟效果最明显,利于原球茎增殖的培养基为MS＋6-BA1．0mg／L＋NAA0．5mg／L,增殖系数为3．97,最佳的生根培养基为MS＋NAA0．2mg／1＋GA1．0mg／1＋香蕉泥150g／L,生根率最高可以达到100％。  相似文献   

伯乐树种子萌发与根组织分化规律的研究   总被引：1，自引：0，他引：1  

李坊贞  刘仁林  刘燕 《赣南师范学院学报》2008,29(3):95-98

伯乐树（Bretschneidera sinensis Hemsl.）为我国特有,分布稀少,是国家重点保护的二级濒危树种.本文阐述了伯乐树种子萌发的基本参数及根的组织分化过程,包括根尖和初生结构的形成.通过根尖的连续纵切片观察到伯乐树根尖的根冠、分生区、伸长区及成熟区四个部分的细胞形态结构,通过根的横切片,观察根的初生结构的形成过程,初生结构由外至内包括表皮、皮层和维管柱三部分.  相似文献   

生物医学工程:一个发展迅速值得关注的交叉学科——生物力学、组织工程学、生物材料与人工器官   总被引：4，自引：0，他引：4  

李恩中  曹河圻  杜生明 《中国科学基金》2006,20(3):153-158

生物医学工程学（Biomedical Engineering，BME）是包含多种技术并相互交叉融合的一门科学。它综合了生物学、医学与工程学的理论和方法，研究生命体的构造、功能、状态和变化，研究新材料、新技术、新仪器设备，用于防病、治病、保护人民健康和提高医学水平。涉及科学领域广泛，除生物学、医学外，还有电子学、微电子学、现代计算机技术、化学、高分子化学、力学、近代物理学、光学、射线学、精密机械和近代高技术，并在不断发展扩大，是各国争相大力发展的高技术之一。本文主要从生物力学、组织工程学、生物材料与人工器官等方面进行探讨。  相似文献   

人牙及骨组织中稀土分布状态的研究   总被引：1，自引：0，他引：1  

程发良  钟广涛 《科技通报》1994,10(6):380-382

测定了人体骨胳、牙齿中的稀土含量，考察了牙齿作为研究人体稀土代谢状况样品的可行性，研究了微量稀土元素在人牙、骨中的分布状态，提出了稀土在人体不同部位骨胳中分布的可能机制。  相似文献   

驱蚊香草不同外植体诱导愈伤组织的研究     

庞发虎 《伊犁师范学院学报》2006,(3):67-69

先后用驱蚊香草的叶片、叶柄、幼嫩顶芽、休眠芽、带皮茎段、去皮茎段等作外植体,进行愈伤组织的诱导.结果表明:在所有的外植体中,以幼嫩顶芽为最好,去皮茎段、叶片次之,愈伤组织诱导率分别为94%、89%和80%;带皮茎段和叶柄的愈伤组织诱导率较低,为25.7%和14.3%左右,休眠芽未诱导出愈伤组织.  相似文献