Cyclooxygenase-2 inhibitor inhibits hippocampal synaptic reorganization in pilocarpine-induced status epilepticus rats     

Zhang HJ  Sun RP  Lei GF  Yang L  Liu CX 《Journal of Zhejiang University. Science. B》2008,9(11):903-915

  相似文献   

A New Potent Inhibitor of Thrombin from the Leech Haemendipsa Yanyuanensis   总被引：1，自引：0，他引：1  

ZHAO Rong le College of Life Sciences  Beijing Normal University  Beijing  China 《上海大学学报(英文版)》2001,(1)

1　Ｉｎｔｒｏｄｕｃｔｉｏｎ　Ｔｈｒｏｍｂｕｓｄｉｓｅａｓｅｏｆｈｅａｒｔａｎｄｂｒａｉｎｂｌｏｏｄｖｅｓｓｅｌｈａｓｂｅｃｏｍｅａｋｉｌｌｅｒｏｆｍａｎ .Ｂｅｃａｕｓｅｏｆｔｈｅｓｉｄｅｅｆｆｅｃｔｓｏｆｈａｅｍｏｒｒｈａｇｅｏｒｔｈｅｓｈｏｒｔｈａｌｆ ｌｉｆｅｉｎｖｉｖｏ ,ｔｈｅｐｒｅ ｓｅｎｔａｎｔｉｃｏａｇｕｌａｎｔｄｒｕｇｓａｒｅｕｎｓａｔｉｓｆｉｅｄ ,ａｎｄｓｅａｒｃｈ ｉｎｇｆｏｒｎｅｗａｎｔｉｃｏａｇｕｌａｎｔｓｗｉｔｈｈｉｇｈｅｒｅｆｆｉｃｉｅｎｔａｎｄｍｏｒｅｓｔａｂｉｌｅｈａ…  相似文献   

结构基因组学与蛋白质核磁共振(NMR)光谱     

李宏  姜怀春 《重庆第二师范学院学报》2006,19(3):25-27,31

阐述了核磁共振光谱在蛋白质结构基因组学研究中的重要作用,包括核磁共振光谱在结构基因组学研究中的优点和缺点,研究技术进展和展望。  相似文献   

抗冻蛋白的抗冻活性及其测定方法   总被引：1，自引：0，他引：1  

邵强  李海峰  刘国生  闫清华 《平原大学学报》2005,22(4):111-113

抗冻蛋白是一类能够保护生物体免受结冰引起伤害的蛋白质，其抗冻活性表现为热滞性和重结晶抑制效应。本文讨论了影响抗冻蛋白活性的因素，介绍了抗冻蛋白热滞活性和重结晶抑制效应的定量测定方法。  相似文献   

Troponin T and Heart Type Fatty Acid Binding Protein (h-Fabp) as Biomarkers in Patients Presenting with Chest Pain     

Lakshmi Lavanya Reddy  Swarup A. V. Shah  Alpa J. Dherai  Chandrashekhar K. Ponde  Tester F. Ashavaid 《Indian journal of clinical biochemistry : IJCB》2016,31(1):87-92

Acute coronary syndrome (ACS) is a term for a range of clinical signs and symptoms suggestive of myocardial ischemia. It results in functional and structural changes and ultimately releasing protein from injured cardiomyocytes. These cardiac markers play a major role in diagnosis and prognosis of ACS. This study aims to assess the efficacy of heart type fatty acid binding protein (h-FABP) as a marker for ACS along with the routinely used hs-TropT. In our observational study, plasma h-FABP (cut-off 6.32 ng/ml) and routinely done hs-Trop T (cutoff 0.1 and 0.014 ng/ml) were estimated by immunometric laboratory assays in 88 patients with acute chest pain. Based on the clinical and laboratory test findings the patients were grouped into ACS (n = 41) and non-ACS (n = 47). The diagnostic sensitivity, specificity, NPV, PPV and ROC curve at 95 % CI were determined. Sensitivity of hs-TropT (0.1 ng/ml), hs-TropT (0.014 ng/ml) and h-FABP were 53, 86 and 78 % respectively and specificity for the same were 98, 73 and 70 % respectively. Sensitivity, specificity and NPV calculated for a cut-off combination of hs-TropT 0.014 ng/ml and h-FABP was 100, 51 and 100 % respectively. These results were substantiated by ROC analysis. Measurement of plasma h-FABP and hs-TropT together on admission appears to be more precise predictor of ACS rather than either hs-Trop T or h-FABP.  相似文献   

Blood zinc protoporphyrin, serum total protein, and total cholesterol levels in automobile workshop workers in relation to lead toxicity: Our experience     

Suneesh Kumar Pachathundikandi  Earaly Thomas Varghese 《Indian journal of clinical biochemistry : IJCB》2006,21(2):114-117

Blood zinc protoporphyrin (ZPP), serum total protein (TP), and total cholesterol (TC) levels in automobile workshop workers in relation to lead toxicity were analysed. In the present study, automobile workshop workers (healthy male workers at an age between 28 and 35 from four major automobile workshops in Kottayam, Kerala State, India) and the control (male healthy adults at an age between 28 and 35 residing at Aymanam, a distant village at Kottayam District, Kerala having reduced or no chance of lead exposure) displayed significant difference in blood lead (BPb) and blood ZZP (BZPP) level. The mean value of BPb in automobile workshop workers was 15.76±0.33 μg/dl, while in the control it was 8.20±0.15 μg/dl. In automobile workshop workers, the mean value of BZPP was 34.2±0.62 μg/dl. The control group exhibited a mean of 11.5±0.22 μg/dl. Automobile workshop workers exhibited significant increase in BZPP was corresponding to the increase in BPb level. The total protein levels estimated in automobile workshop workers showed significant decrease compared to control individuals, but was within the reference range of healthy individuals. The mean value of TP level in automobile workshop workers and control was 6.9±0.13 g/dl and 7.71±0.18 g/dl, respectively. There was no significant difference in blood haemoglobin (BHb) level among the automobile workshop workers and control. The serum TC level in automobile workshop workers showed significant decrease compared to the control individuals, but was with in the reference range of healthy individuals. The mean level of serum TC in automobile workshop workers was 162.00±3.44 mg/dl and the same in control was 172.86±4.32 mg/dl. The present study affirms occupational lead toxicity in automobile workshop workers and its effect on serum protein and cholesterol levels.  相似文献   

QTL analysis for some quantitative traits in bread wheat     

Pushpendra KG  Harindra SB  Pawan LK  Neeraj K  Ajay K  Reyazul RM  Amita M  Jitendra K 《Journal of Zhejiang University. Science. B》2007,8(11):807-814

Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain protein content (GPC) sug- gested that the major part of genetic variation for this trait is due to environmental interactions. In contrast, pre-harvest sprouting tolerance (PHST) was controlled mainly by main effect QTL (M-QTL) with very little genetic variation due to environmental interactions; a major QTL for PHST was detected on chromosome arm 3AL. For grain weight, one QTL each was detected on chromosome arms 1AS, 2BS and 7AS. QTL for 4 growth related traits taken together detected by different methods ranged from 37 to 40; nine QTL that were detected by single-locus as well as two-locus analyses were all M-QTL. Similarly, single-locus and two-locus QTL analyses for seven yield and yield contributing traits in two populations respectively allowed detection of 25 and 50 QTL by composite interval mapping (CIM), 16 and 25 QTL by multiple-trait composite interval mapping (MCIM) and 38 and 37 QTL by two-locus analyses. These studies should prove useful in QTL cloning and wheat improvement through marker aided selection.  相似文献   

Decolorization Kinetics of Sludge Protein Solution by ~（60）Coγ-Ray Irradiation/H_2O_2 Oxidation     

相玉琳  张卫江  郑浩 《天津大学学报(英文版)》2011,17(1):45-50

In order to carry out decolorization, sludge protein solution, a dark brown close to black solution from activated sludge, was subjected to ⁶⁰Co γ-ray irradiation in the presence of hydrogen peroxide. UV/Vis spectrophotometric method was used to investigate the effect of H2O2 on the coloration apparent kinetics and rate constants of sludge protein solution under γ-ray irradiation. In addition, the effects of irradiation dose, initial sludge protein solution concentration, and pH value on the decolorization efficiency of sludge protein solution were studied. Results showed that the decolorization apparent kinetics of sludge protein solution was a first-order reaction. The solution decolorization percentage increased with the increase of irradiation dose or the decrease of initial sludge protein solution concentration. The examination results of pH value showed that the sludge protein solution could be more efficiently decolorized in alkaline media than in acid media. Moreover, sensory evaluation and foamability analysis indicated that irradiated samples under H2O2 oxidation showed better sensory score and foamability.  相似文献   

人工锌指核酸酶的电子设计与结构模拟     

张勇  蒋泓  邓廷贤  粟文俊  杨莉珊  唐冬生 《实验室研究与探索》2011,30(10)

以电子设计用于猪的多位点基因打靶的人工锌指核酸酶为例,探索如何电子设计优化理想的人工锌指核酸酶,以减少人工锌指核酸酶的毒性。采用OPEN电子设计方法,生物信息学方法的预测分析ZFP的立体结构,设计优化合适的连接体和间隔体组合,并通过突变优化Fok I异源二聚体。采用OPEN法设计三指ZF序列,并连接为ZFP序列,设计出长度为6 bp且富含AT碱基的spacer,加上长度为4个氨基酸残基的inter-domain linker,连接FokⅠ切割结构域双突变子异源二聚体,并通过各种软件工具对ZFN的空间结构进行模拟预测,所设计得到的ZFN的有效率超过50%。探索获得电子设计理想ZFN的方案,即特异性结合DNA的锌指蛋白、最佳的linker和spacer的长度与组合以及优化的特异性切割的Fok I异源二聚体切割结构域。  相似文献   

蛋白质序列的一种新的二维图形表示     

李丹丹  赵会仁  刘璐  张振 《南阳师范学院学报》2011,10(6):17-20

基于氨基酸的五字母模型,提出蛋白质序列的一种二维图形表示方法,然后证明这种图是非退化的.最后用该方法给出了8个物种的β球蛋白序列的图形表示.  相似文献