不同训练阶段尿异常的比较     

李泱  张洁  刘文  侯菊花 《中国体育科技》2000,36(5)

对河南省中长跑运动员参加 8运会前的 3次高原训练和平原强化训练以及达标赛及 8运会比赛分别进行了运动后晨尿的检测和分析。结果发现 :1)尿异常的发生率以蛋白质 (PRO)、尿胆原 (URO)为较高 ,可作为尿检测的敏感指标来评价运动负荷的科学性和身体对训练的适应能力。 2 )尿液 PRO和 URO与运动强度之间高度相关 (r=0 .71,r =0 .85 )。 3) PRO值的高低与运动强度关系密切 ,同等训练强度时 PRO的排泄量高原显著大于平原。  相似文献   

Molecular basis of familial hypercholesterolemia: An Indian experience     

Ashavaid T. F.  Altaf A. K.  Nair K. G. 《Indian journal of clinical biochemistry : IJCB》2000,15(1):11-16

Modification of platelet proteins by free radicals and glycation has been studied in the present work, as anin vitro model. The results of the two parameters, SDS-PAGE and carbonylation study are quite corroborative. We observed that the inducers like ferrous sulphate, ascorbate (mainly in supraphysiological concentration) and glucose attack the protein in a dose dependent manner, of which ferrous sulphate is most potent. Proteins from aged and degenerative conditions like malignancy and diabetes mellitus have suffered greater damage than normal adult and foetal proteins. The individual life expectancy in terms of biological versus chronological age may also be worked out from the individual stress level.  相似文献   

Diagnosis of alpha-1-antitrypsin deficiency by serum protein electrophoresis     

V. G. Justin  T. Venkatesh 《Indian journal of clinical biochemistry : IJCB》2000,15(2):171-173

Alpha-1-antitrypsin deficiency is a hereditary disease leading to hepatitis and cirrhosis. It is the most common genetic cause of liver disease in children which must inherit the tendency from both parents to develop. It acquires the highest priority in the differential diagnosis in a child with chronic liver disease. In this case report we substantiate the role of serum protein electrophoresis, in diagnosing alpha-1-antitrypsin deficiency.  相似文献   

生物形态进化和转录调节基因     

金振华 《中国科学基金》2000,14(3):143-146

通过阐述转录调节基因与进化的关系,列举了转录调节基因的改变是驱动生物进化的 诸多论据及检验途径。  相似文献   

抗阻运动通过刺激骨骼肌FSTL1分泌抑制心梗大鼠心肌细胞凋亡及其机制探讨     

郝美丽  席悦  田振军 《体育科学》2020,(1):67-78

目的:探讨抗阻运动通过刺激骨骼肌卵泡抑素样蛋白1(Follistatin-like protein 1,FSTL1)分泌,抑制心梗(Myocardial Infarction,MI)大鼠心肌细胞凋亡及其机制。方法:动物实验:结扎SD大鼠左冠状动脉前降支制备MI模型,术后随机分为5组,分别为假手术组(S)、心梗安静对照组(MI)、心梗+抗阻运动组(MR)、心梗+腺相关病毒空载体组(MV)、心梗+FSTL1腺相关病毒载体组(MF),每组10只,其中S组只穿线不结扎。术后1周,MR组进行为期4周的爬梯抗阻运动,MV组和MF组于左后肢胫骨前肌分别注射腺相关病毒空载体和FSTL1腺相关病毒载体。训练结束后次日,腹腔麻醉,测定心功能,摘取心脏和左后肢胫骨前肌。Masson染色观察并计算心肌胶原容积百分比(CVF%);TUNEL检测分析心肌细胞凋亡;Western Blotting实验测定骨骼肌和血清FSTL1蛋白含量,心肌FSTL1、DIP2A、p-Akt/Akt、p-mTOR/mTOR、Bcl2/Bax蛋白表达。细胞实验:H9C2细胞分为8组,即H9C2对照组、H9C2+LPS(脂多糖:Lipopolysaccharide,LPS)组、H9C2+LPS+AICAR(AMPK激动剂AICAR)组、H9C2+LPS+LY294002(PI3K抑制剂LY294002)组、H9C2+LPS+AICAR+LY294002组、H9C2+LPS+rhFSTL1组、H9C2+LPS+rhFSTL1+AICAR组、H9C2+LPS+rhFSTL1+LY294002组。TUNEL检测H9C2细胞凋亡,CCK8检测H9C2细胞存活率,Western Blotting实验检测细胞FSTL1、DIP2A、p-Akt/Akt、p-mTOR/mTOR、Bcl2/Bax蛋白表达。结果:MI后大鼠骨骼肌FSTL1基因和蛋白表达降低,心肌FSTL1基因表达无显著变化,心肌和血清FSTL1蛋白水平显著升高,心肌细胞凋亡和心肌纤维化显著增加,心功能下降。抗阻运动或胫骨前肌注射FSTL1腺相关病毒载体后,骨骼肌、血清和心肌FSTL1蛋白水平均显著升高,心肌FSTL1受体DIP2A、p-Akt、p-mTOR、Bcl2/Bax蛋白表达均显著上调,心肌细胞凋亡和心肌纤维化减少,心功能显著改善,且抗阻运动显著上调骨骼肌FSTL1基因表达,但心肌FSTL1基因表达无显著性差异。FSTL1和AICAR干预均显著抑制LPS诱导的H9C2细胞凋亡,增加FSTL1、DIP2A、p-Akt、p-mTOR、Bcl2/Bax蛋白表达水平和细胞存活率,LY294002干预与上述作用相反。结论:在抗阻运动上调MI大鼠心肌FSTL1表达抑制心肌细胞凋亡过程中,骨骼肌源性FSTL1发挥重要作用,骨骼肌源性FSTL1可通过血液循环到达心脏,与其受体DIP2A结合,激活下游Akt-mTOR信号通路,抑制心肌细胞凋亡,降低心肌纤维化,改善MI大鼠心功能。  相似文献   

Cow's milk as a post-exercise recovery drink: implications for performance and health     

Lewis J. James  Emma J. Stevenson  Penny L. S. Rumbold  Carl J. Hulston 《European Journal of Sport Science》2019,19(1):40-48

Abstract

Post-exercise recovery is a multi-facetted process that will vary depending on the nature of the exercise, the time between exercise sessions and the goals of the exerciser. From a nutritional perspective, the main considerations are: (1) optimisation of muscle protein turnover; (2) glycogen resynthesis; (3) rehydration; (4) management of muscle soreness; (5) appropriate management of energy balance. Milk is approximately isotonic (osmolality of 280–290?mosmol/kg), and the mixture of high quality protein, carbohydrate, water and micronutrients (particularly sodium) make it uniquely suitable as a post-exercise recovery drink in many exercise scenarios. Research has shown that ingestion of milk post-exercise has the potential to beneficially impact both acute recovery and chronic training adaptation. Milk augments post-exercise muscle protein synthesis and rehydration, can contribute to post-exercise glycogen resynthesis, and attenuates post-exercise muscle soreness/function losses. For these aspects of recovery, milk is at least comparable and often out performs most commercially available recovery drinks, but is available at a fraction of the cost, making it a cheap and easy option to facilitate post-exercise recovery. Milk ingestion post-exercise has also been shown to attenuate subsequent energy intake and may lead to more favourable body composition changes with exercise training. This means that those exercising for weight management purposes might be able to beneficially influence post-exercise recovery, whilst maintaining the energy deficit created by exercise.  相似文献   

运动对骨骼肌收缩蛋白的影响   总被引：2，自引：0，他引：2  

黄海 《体育科学》1993,13(3):58-60,65

本文观察运动和不运动组小白鼠骨骼肌收缩蛋白含量,Mg—ATPase 活性以及对 Ca~(2+)感受性的生理特性。我们发现:运动不仅可以使骨骼肌收缩蛋白含量增加,而且活性化高的蛋白同时合成,肌动蛋白和肌球蛋白的相互收缩反应说明运动可使骨骼肌对 Ca~(2+)的感受性明显改善,肌动球蛋白 Mg—ATPase 的活性增高。结果表明:骨骼肌收缩蛋白在质和量的变化,可能是由肌动球蛋白 Mg—ATPase 的活性增高而引起。  相似文献   

疏筋壮骨功提高人体机能的医学观察   总被引：1，自引：0，他引：1  

张广德  周英男  孙卫星  赖柳明  隆璐娟  郭秀英  屈智琼 《北京体育大学学报》1993,(1)

对28例练习疏筋壮骨功者3个月前后的部分指标进行了测定,论证了提高人体有关系统机能的方法及原因,为指导人们更好地练功提供了参考依据。  相似文献   

Study on properties of residue-residue contacts in protein     

王向红  柯见洪  郑亦庄  陈爱  徐银香 《Journal of Zhejiang University. Science. B》2004,(8)

INTRODUCTION Recognition of protein folding from aminoacid sequence is a challenging task. The structureand stability of proteins from different fold aremainly dictated by inter-residue interactions. In-formation on the distribution of contacts shown by20 amino acid residues can be used to predict thefolding type of each protein (Chan and Dill, 1998;Zhang and Kim, 2000; Gromiha, 2001; Gromihaand Selvaraj, 1999; Miyazawa and Jernigan, 1985;1996; Bahar et al., 1997; McDonald and …  相似文献   

杂交水稻离体叶片衰老与蛋白质代谢的关系   总被引：3，自引：0，他引：3  

宋松泉  苏卫珍  彭晓南 《中山大学学报论丛》1995,(1)

杂交水稻离体叶片衰老时，叶片中的叶绿素和蛋白质含量下降．游离氨基酸含量增加．氨肽酶和内肽酶的活性显著上升，说明在衰老叶片中氨肽醇和内肽酶的作用是蛋白质降解的主要原因．  相似文献