The Plasma DNA Concentration as a Potential Breast Cancer Screening Marker     

Orathai Tangvarasittichai  Watchiravut Jaiwang  Surapon Tangvarasittichai 《Indian journal of clinical biochemistry : IJCB》2015,30(1):55-58

Increased levels of plasma DNA have frequently been noticed in the blood plasma of cancer patients. The possibility of using plasma DNA level as the indicator of tumor stage in breast cancer was investigated in plasma samples obtained from 100 breast cancer patients and 100 healthy women who were included as controls. Circulatory plasma free DNA was extracted from plasma samples and quantified by fluorometer. The median concentration of plasma DNA in the plasma samples from breast cancer patients classified by TNM staging system as stage I, II, III, IV and breast surgical patients were 0.5, 235, 422, 1,280 and 0.5 ng/ml, respectively. The level of plasma DNA in the stage II- IV group was significantly higher than those in the surgical group with breast cancer and control group (P value < 0.001). The plasma DNA concentration in stage II, III and IV of breast cancer were higher when compared with healthy group. These tumor size, TNM stage and metastasis were significantly correlated with plasma DNA. The cut point of 120 ng/ml was early screening and treatment follow up breast cancer.  相似文献   

Science Letters: Quantification of Helicobacter pylori levels in soil samples from public playgrounds in Spain     

Leonardo Martín P 《Journal of Zhejiang University. Science. B》2010,11(1)

Helicobacter pylori are ubiquitous Gram-negative bacteria with a high estimated level of infection in the world populations, but a majority of the infected persons are asymptomatic. This pathogen has been classified by the World Health Organization as a class I carcinogen and recognized as the causal agent of most peptic ulcers and chronic gastritis that might lead to stomach cancer. Although not all the transmission pathways of these bacteria into humans have been properly identified, enough data have sugg...  相似文献   

拓扑异构酶Ⅰ抑制剂介导的DNA损伤、修复和检验点应答     

马云彤 《西安文理学院学报》2010,13(1):44-47

拓扑异构酶Ⅰ在DNA翻译和转录过程中催化单链DNA的断裂和连接,从而松弛DNA超螺旋,促进复制与转录的进行．拓扑异构酶Ⅰ抑制剂能够阻断DNA链的再连接,结果导致TopⅠ断裂复合物的积累,抑制复制和转录,造成DNA损伤,从而激活DNA损伤检验点,抑制细胞生长和诱发凋亡。喜树碱是最早发现、也是最重要和最广泛使用的拓扑异构酶Ⅰ抑制剂．细胞周期中DNA损伤、修复和检验点应答的分子机制是目前生命科学研究热点,而喜树碱已成为进行相关研究的重要的药理学工具．  相似文献   

氨基酸席夫碱金属配合物的研究进展     

张然 《滨州学院学报》2010,26(3):74-78

概述了近年来国内外在氨基酸席夫碱金属配合物领域的研究,探讨了氨基酸席夫碱配合物与DNA之间作用机理,并对该领域的研究前景作出展望.  相似文献   

长期运动训练对青年男性淋巴细胞凋亡及线粒体DNA修复酶的影响     

代志军  朱荣 《西安体育学院学报》2012,29(3):350-354

目的探究长期运动训练对青年男性淋巴细胞凋亡及线粒体DNA(mtDNA)修复酶(8-氧鸟嘌呤DNA糖基化酶,OGG1)的影响及可能机制。方法训练组为20名男子足球专业运动员,对照组为20名男子大学生。2组均在功率自行车上完成递增负荷力竭运动。运动前和运动后即刻采集静脉血。流式细胞法检测淋巴细胞凋亡率,比色法检测超氧阴离子和羟自由基含量和Caspase-3,Caspase-9活性,高效液相色谱法检测mtDNA中8-氧鸟嘌呤(8-oxodG)含量,Western-blotting法检测线粒体OGG1蛋白(mtOGG1)表达水平。结果运动前和运动后,训练组与对照组比较,超氧阴离子、羟自由基、Caspase-3,Caspase-9活性、8-oxodG降低,OGG1升高;运动后,训练组与对照组比较,淋巴细胞凋亡率降低。对照组和训练组在运动后与运动前比较,淋巴细胞凋亡率、超氧阴离子、羟自由基、Caspase-3,Caspase-9活性和8-oxodG均升高,但训练组升高幅度低于对照组;运动后对照组OGG1降低,而训练组OGG1升高。结论一次性力竭运动诱导产生高水平ROS,并抑制mtOGG1表达,使mtDNA氧化损伤,促进淋巴细胞凋亡;长期耐力训练可通过抑制ROS生成,并提高mtOGG1表达,抑制运动性淋巴细胞凋亡。  相似文献   

Evaluation of Quality of DNA Extracted from Buccal Swabs for Microarray Based Genotyping     

Alex Livy  Sayhean Lye  Chahil K. Jagdish  Nurul Hanis  Velapasamy Sharmila  Lian Wee Ler  Bagali Pramod 《Indian journal of clinical biochemistry : IJCB》2012,27(1):28-33

Buccal cell usage has been shown by many to be a cost effective and safe method to isolate DNA for various biological experiments especially large epidemiological studies (Garcia-Closas et al. Cancer Epidemiol Biomarkers Prev 10:687–696, 2001). Non-invasive DNA collection methods are preferred over phlebotomy in order to increase study participation and compliance in research centers and for sick patients in hospital settings. There have been conflicting reports about the methodology and results obtained from using buccal DNA. It is not very clear if phlebotomy can be confidently replaced by buccal cell DNA. It is often left for the user to take an intelligent decision. To address this issue, we compared the performance of buccal and blood DNA from same subjects in a genotyping experiment and this paper reports the results. Cotton swab derived buccal cells were scraped from the inner side of cheeks from 16 subjects, and blood was also drawn from the same 16 subjects participating in a genotypic association study of a lipid disease. The DNA quality was assessed by resolving on agarose gels, checking purity (A260/A280) and finally by microarray hybridization. This study showed that DNA degradation affects the total yield and performance of the buccal DNA when compared to the blood DNA in microarray based genotyping. Genotyping results can be seriously compromised if care is not taken to check the quality and yields of such specimens.  相似文献   

企业文化中心法则构建机制研究     

马晓苗  石冠峰 《科技管理研究》2012,32(7):227-230

结合生物中心法则,提出了企业文化中心法则。企业文化的三个层次——核心价值观、制度/技术文化以及行为/形象文化分别对应于生物大分子DNA、RNA和蛋白质。企业文化三层次间也存在着信息复制、转录和转译的过程。企业文化中心法则的构建,为我们探讨企业文化层次要素间的信息传递、企业文化的运行演化规律以及构建良好的企业文化提供了新的视角和思路。  相似文献   

Questioning Race: Ancestry DNA and Dialog on Race     

《Communication monographs》2012,79(2):271-290

Human genetics and racial identity converge pointedly in the family narrative. Until recently, genetics, racial identity, and family narrative were all rather malleable concepts in the public arena. All were presented in anecdotal form for the most part, and were often based on certain social conventions. The interjection of popularly available ancestry DNA data adds an additional piece of information to the discussion of genetics, race, and narrative. Using the framework of both narrative theory and theory of social construction, this work uses quantitative and qualitative data to explore how individuals react to ancestry DNA findings and to consider if and how this information will change narratives, behaviors, and perspectives. We also explore whether one's racial identification makes a difference in initial accuracy and if there is a difference among racial groupings in terms of change in census identification based on knowledge of their DNA profile.  相似文献   

从“DNA的粗提取与鉴定”中体验乐趣     

张云娟  刘家华 《科教文汇》2010,(33):106-106

生物是以实验为基础的学科,有趣简单的实验能调动学生学习的积极性。DNA主要存在于细胞核中,只要有细胞核的生物都会含有DNA。用简单易得的实验材料,做相对简单的实验,能让学生在DNA的粗提取与鉴定中感受实验的乐趣。  相似文献   

脱氧核苷酸钠临床研究综述     

张小月  接艳秀 《黑龙江科技信息》2010,(8):188-188

结合实际,系统地阐述了脱氧核苷酸钠的性质和临床用途。  相似文献